Tribal Warfare: Killing of pathogen N. gonorrhoeae by commensal N. elongata

 描述（由申请人提供）：在15种存在于人体粘膜表面的奈瑟氏菌中，只有一种是病原体;其他的是构成我们正常植物群一部分的寄生菌。鉴于最近的报道，大肠杆菌可以抑制病原体定植，我们确定是否大肠杆菌细长奈瑟菌具有拮抗病原体淋病奈瑟菌在体外和体内的能力。总结我们的发现，细长奈瑟氏球菌显著降低了N。淋病在体外共培养期间。培养基中的阿根是细长奈瑟氏菌染色体DNA，而细长奈瑟氏菌的致死原是染色体DNA。淋病需要其以IV型菌毛/DUS依赖性方式被病原体摄取。此外，N.伸长加速了N的清除。淋病病毒我们假设N.伸长的基因组含有一个或多个基因座，当被N.淋病对病原体是致命的我们进一步假设，吸收N。延长DNA的N.淋病是病原体从小鼠体内迅速清除的机制。我们提出了两个具体的目的来测试这些假设在体外和体内。

 DESCRIPTION (provided by applicant): Of the15 species of Neisseria that inhabit human mucosal surfaces, only are pathogens; the others are commensals that form part of our normal flora. In light of recent reports that commensal bacteria can inhibit pathogen colonization, we determined whether commensal Neisseria elongate has the ability to antagonize pathogen Neisseria gonorrhoeae in vitro and in vivo. To summarize our findings, Neisseria elongate dramatically lowers the viability of N. gonorrhoeae during co--‐culture in vitro. The lethal agen, which is in the medium, is Neisseria elongate chromosomal DNA, and killing of N. gonorrhoeae requires its uptake by the pathogen in a Type IV pilus/DUS dependent manner. Moreover, N. elongate accelerates the clearance of N. gonorrhoeae from mice. We hypothesize that the N. elongate genome harbors a locus/loci which, when taken up by N. gonorrhoeae, is lethal to the pathogen. We further hypothesize that uptake of N. elongate DNA by N. gonorrhoeae is the mechanism by which the pathogen is rapidly cleared from mice. We propose two Specific Aims to test these hypotheses in vitro and in vivo..