权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Histology/lmage Analysis

组织学/图像分析

基本信息

批准号：
9085484
负责人：
John D. Houle
金额：
$ 7.48万
依托单位：
DREXEL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-15 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9085484
关键词：
Acute Antibodies Area Behavior Buffers Cell Adhesion Molecules Cells Chronic Conflict (Psychology)Core Facility Cytoskeleton Data Correlations Databases Electrophysiology (science)Equipment Equipment and Supplies Equipment and supply inventories Event Extracellular Matrix Faculty Felis catus Goals Grant Growth Histology Homing Human Resources Ice Image Image Analysis Immunohistochemistry In Situ Hybridization Individual Injury Innovative Therapy Instruction Internships Label Lesion Maintenance Mediating Methods Microscope Modeling Molecular Morphology Natural regeneration Neuroglia Neuromodulator Neurotransmitters Policies Postdoctoral Fellow Procedures Process Quality Control Rattus Recovery Regulation Research Personnel Resources Schedule Site Specimen Spinal Cord Spinal cord injury Staining method Stains Structure Students Systems Analysis Teaching Method Techniques Testing Time Tissues Training Transplantation Update Visit Work animal tissue axon growth cell type cost experience experimental analysis functional outcomes graduate student improved meetings member novel operation programs repaired research study tissue fixing tissue preparation tissue processing treatment effect treatment strategy wasting

项目摘要

CORE D - Histology and Image Analysis Core - Theresa Connors - PI The goal of the Histology and Image Analysis Core is to identify and quantify the anatomical substrates of functional outcomes tested in the Electrophysiological and Behavior Cores and in the individual projects. This core has evolved over 20+ years to insure standardized throughput of the large quantity of specimens generated by individual projects of the PPG. We have found that training staff from each project and providing a well organized and stocked facility where work can be performed with guidance from core staff is the most efficient way to utilize resources. The Histology and Image Analysis Core maintains a database of all tissue processing, histological and immunohistochemical (IHC) procedures used by PPG personnel. In addition, the core staff oversees training on and maintenance of our image acquisition and analysis systems. The core director and technician are skilled in working with a wide variety of antibodies and procedures used to identify neurotransmitters and neuromodulators, cytoskeleton components, extracellular matrix and adhesion molecules, growth factors, and non-neuronal cells as well as fiuorescence and DAB labeling techniques. Core personnel are also well versed in the operation and application of the image analysis microscopes and softiA/are. The core strives to maintain quality control of materials and procedures used in all PPG projects by: requiring training prior to using core resources, providing stocks of all routinely used buffers and solutions, regularly updating the database of antibodies and procedures and providing guidance in the planning and execution of projects in the core lab. Testing and implementation of new techniques is an essential Core function. This has increased the efficiency of the PPG Histology and Image Analysis Core, reducing start-up time for new procedures while also reducing duplication and waste. With centralized training, equipment, supplies and a database for methods, the Histology and Image Analysis Core has evolved as the model for other Core Labs in the Program Proiect

CORE D -组织学和图像分析CORE - Theresa Connors - PI