Cellular and Molecular Biology

细胞和分子生物学

• 批准号: 8828804
• 负责人: John D. Houle
• 金额: $ 3.45万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-15 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8828804
• 关键词: Anatomy; Biological; Cell Line; Cell Maintenance; Cell Transplantation; Cells; Cellular biology; Cloning Vectors; Complementary DNA; Core Facility; Culture Media; Data Analyses; Databases; Doctor of Philosophy; Enzyme-Linked Immunosorbent Assay; Enzymes; Equipment; Experimental Designs; Fibroblasts; GRP gene; Genetic; Growth; Individual; Injury; Instruction; Label; Mediating; Modeling; Molecular; Molecular Analysis; Molecular Biology; Molecular and Cellular Biology; Neurobiology; Neuroepithelial Cells; Neurons; Plasmids; Population; Procedures; Quality Control; Reagent; Records; Research Personnel; Serum; Site; Spinal cord injury; Structure of thyroid parafollicular cell; Students; System; Technical Expertise; Testing; Therapeutic; Time; Tissues; Training; Transfection; Transplantation; Variant; Viral; Virus; Western Blotting; adeno-associated viral vector; cellular transduction; computerized; cost effective; genetically modified cells; immortalized cell; improved; nerve stem cell; programs; recombinant virus; repaired; repository; research study; therapeutic gene; tissue culture; treatment strategy; virology

CORE C: Cell and Molecular Biology - Ying Jin, Ph.D. - PI This Core has existed for 10 years within the Department of Neurobiology and Anatomy to facilitate the molecular analysis of cells and tissues in the SCI site and within transplants. Most importantly, the core prepares cells for grafting experiments in the individual projects. All projects will use the Cell and Molecular Core. The facility isolates, expands and stores primary fibroblasts, neural stem cells and cell lines as required. We genetically modify cells by viral transduction or transfection and provide quality control for all cells and reagents used in the core. The staff of the Cell and Molecular Core provides assistance for experimental design and data analysis. The core has developed a computerized ordering system in which individual investigators & staff request control, labeled or genetically modified cells for transplantation. Our records show that over the past 5 years the core has provided over 3 x 1 0 ^ cells, including unmodified and modified fibroblasts, neuronal and glial restricted precursors (NRP and GRP), neuroepithelial cells (NEP) and cells from immortalized cell lines. The core maintains viruses and transduced cell stocks in long-term storage and serves as a database and repository for cDNA clones, vectors, viruses and cells. Efficient and cost-effective ordering of tissue culture and molecular biology supplies as well as large scale testing of new sera and improved growth media, enzymes, kits or equipment needed for molecular analysis (thermocyclers, Q-PCR) are key core functions. We are also continuously developing new improved plasmids and viruses and have recently added and adeno-associated virus vector (AAV) as an option to our program. This will allow us to specifically transfect graft neural stem cells with growth promoting and therapeutic factors to be delivered at or near the site of injury.

核心C：细胞和分子生物学- Ying Jin，Ph.D. - Pi 这个核心已经在神经生物学和解剖学系存在了10年，以促进 SCI部位和移植物内细胞和组织的分子分析。最重要的是，核心 为个别项目的移植实验准备细胞。所有项目将使用细胞和分子 核心该设施分离、扩增和储存原代成纤维细胞、神经干细胞和细胞系， 必需的.我们通过病毒转导或转染对细胞进行遗传修饰，并为所有细胞提供质量控制。 核心中使用的细胞和试剂。细胞和分子核心的工作人员提供援助， 实验设计和数据分析。核心开发了一个计算机化的订购系统， 个别研究者和工作人员要求对照、标记或遗传修饰的细胞用于移植。我们 记录显示，在过去的5年里，核心提供了超过3 × 104个细胞，包括未经修改的和 修饰的成纤维细胞、神经元和胶质限制性前体细胞（NRP和GRP）、神经上皮细胞（NEP） 和来自永生化细胞系的细胞。该核心长期维持病毒和转导的细胞储备， 存储器，并用作cDNA克隆、载体、病毒和细胞的数据库和储存库。高效和 具有成本效益的组织培养和分子生物学用品订购以及新的大规模测试 血清和分子分析所需的改良生长培养基、酶、试剂盒或设备（热循环仪， Q-PCR）是关键的核心功能。我们还在不断开发新的改良质粒和病毒 并且最近增加了腺相关病毒载体（AAV）作为我们计划的一个选择。这将 使我们能够特异性地用生长促进因子和治疗因子移植神经干细胞， 在受伤部位或附近进行。