Development of Reagents for Sequencing Proteins and Protein Fragments on a Next-Generation DNA Sequencer

摘要 下一代测序（NGS）已经改变了基因组学领域，并使广泛的 在生物学、转化和最近的临床研究中采用该技术。的 与NGS相当的蛋白质组学尚未实现。因此，本提案的目标是 开发高度可扩展的数字化蛋白质分析技术。该方法具有广泛的适用性， 极大地改善了当前方法的成本、灵敏度、可用性和吞吐量。

ABSTRACT Next generation sequencing (NGS) has transformed the field of genomics and enabled broad adoption of the technology in biological, translational, and most recently clinical research. The proteomics equivalent to NGS has not yet been realized. As such, the goal of this proposal is to develop a highly-scalable digital protein analysis technology. The approach is broadly applicable and greatly improves upon cost, sensitivity, usability, and throughput of current approaches.