Development of Reagents for Sequencing Proteins and Protein Fragments on a Next-Generation DNA Sequencer

开发用于在下一代 DNA 测序仪上对蛋白质和蛋白质片段进行测序的试剂

• 批准号: 9679623
• 负责人: KEVIN L GUNDERSON
• 金额: $ 71.83万
• 依托单位: ENCODIA, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-05 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9679623
• 关键词: Adoption; Applied Research; Area; Automation; Basic Science; Binding; Bioinformatics; Biological; Biological Assay; Biological Sciences; Chemistry; Clinical Research; Code; Complex; Computer software; DNA; DNA Sequence; Development; Drug Targeting; Enzymes; Event; Generations; Genomics; Goals; Immobilization; Industry; Label; Length; Ligation; Maps; Methods; Modeling; Molecular; Peptide Sequence Determination; Peptides; Phase; Polymerase; Preparation; Protein Analysis; Protein Engineering; Protein Fragment; Proteins; Proteomics; Reagent; Sampling; Signal Transduction; Solid; Surface; System; Technology; Titrations; Trypsin; Writing; cost; digital; drug development; high throughput analysis; improved; innovation; next generation; next generation sequencing; optical imaging; single molecule; usability; user-friendly

ABSTRACT Next generation sequencing (NGS) has transformed the field of genomics and enabled broad adoption of the technology in biological, translational, and most recently clinical research. The proteomics equivalent to NGS has not yet been realized. As such, the goal of this proposal is to develop a highly-scalable digital protein analysis technology. The approach is broadly applicable and greatly improves upon cost, sensitivity, usability, and throughput of current approaches.

摘要 下一代测序（NGS）已经改变了基因组学领域，并使广泛的 在生物学、转化和最近的临床研究中采用该技术。的 与NGS相当的蛋白质组学尚未实现。因此，本提案的目标是 开发高度可扩展的数字化蛋白质分析技术。该方法具有广泛的适用性， 极大地改善了当前方法的成本、灵敏度、可用性和吞吐量。