Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing.
Design and directed evolution of an 'Edmanase' enzyme for high-throughput peptide sequencing.
批准号:
10080672
负责人:
KEVIN L GUNDERSON
金额:
$74.97万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-13 至 2022-08-31
关键词:
Amino AcidsApplied ResearchAreaBar CodesBasic ScienceBiological AssayBiological SciencesChemicalsCleaved cellClinical ResearchCouplingDNADNA LibraryDNA sequencingDirected Molecular EvolutionDrug TargetingEngineeringEnzymesGoalsImmobilizationLabelLibrariesMedical ResearchModificationMolecularN-terminalPeptide Sequence DeterminationPeptidesPeriodicityPhaseProcessProtein AnalysisProteinsProteomeReagentSurveysTechnologyVariantbasebiological researchcostcost effectivedesigndigitaldrug developmentgenetic selectionhigh throughput analysisimprovedinstrumentnovelprototypescaffoldscreeningsingle moleculeusabilityuser-friendly
中文摘要
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英文摘要
ABSTRACT
More accessible and cost-effective technologies for analysis of the proteome would
accelerate biological and medical research. The goal of this proposal is to develop
enzymatic reagents to enable creation of a highly-scalable digital protein analysis
technology. The approach is broadly applicable and greatly improves upon cost,
sensitivity, usability, and throughput of current approaches.
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会议论文
Development of Reagents for Sequencing Proteins and Protein Fragments on a Next-Generation DNA Sequencer
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批准号:9679623
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项目类别:
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资助金额:$71.83万
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财政年份:2016
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负责人:KEVIN L GUNDERSON
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依托单位:
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批准号:6790133
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项目类别:
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资助金额:$19.5万
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财政年份:2004
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依托单位:
High resolution DNA copy/LOH measurements on WGG arrays
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批准号:6934807
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项目类别:
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资助金额:$58.4万
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财政年份:2003
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负责人:KEVIN L GUNDERSON
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依托单位:
High resolution DNA copy/LOH measurements on WGG arrays
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批准号:7047919
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项目类别:
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资助金额:$49.07万
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财政年份:2003
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负责人:KEVIN L GUNDERSON
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依托单位:
Representational analysis of DNA copy number/methylation
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批准号:6691586
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项目类别:
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资助金额:$9.97万
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财政年份:2003
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负责人:KEVIN L GUNDERSON
-
依托单位:
海外基金