Direct Deposition of Polymer Fibers for Use as a Surgical Sealant

 描述（由申请人提供）：组织重建和切口和伤口闭合与几乎所有手术干预和创伤性损伤相关。该项目旨在开发一种医用密封剂，以降低成本，炎症反应和疾病传播风险，同时改善手术结果。目前的临床选择受到高成本、差的材料性能和生物相容性问题的组合的影响。利用一种称为溶液吹塑纺丝的技术，聚合物纤维可以直接沉积在任何表面上。溶液喷吹纺丝有可能成为外科手术和生物材料制造中的有力工具。本提案的总体目标是研究聚（乙二醇）（PEG）和聚（乳酸-共-乙醇酸）（PLGA）的溶液吹塑纺丝聚合物共混物在肠吻合模型中用作外科密封剂的用途。本研究的假设是：溶液吹纺PLGA/PEG混合物将优于现有的密封剂策略，从而获得更好的外科手术结局。该提案旨在利用马里兰州大学功能性高分子实验室（UMD）和谢赫扎耶德儿童外科创新研究所的互补专业知识和设施。本研究的具体目的是：1.粘合剂优化、表征和体外评估2.聚合物功能化以改善粘附性和体外表征3.吹纺聚合物密封剂在急性和慢性临床前模型中的体内临床前有效性和生物相容性。这项工作旨在将材料特性和沉积条件与体外和体内功效相关联，并将导致对有效手术材料开发的更深入了解。最终，使用溶液吹塑纺丝将聚合物纤维结构直接沉积到任何表面上的成功开发可能导致在各种外科应用和生物材料制造中的临床可转化方法。

 DESCRIPTION (provided by applicant): Tissue reconstruction and closure of incisions and wounds is pertinent to almost all surgical interventions and traumatic injuries. This project aims to develop a medical sealant that reduces cost, inflammatory response and disease transmission risk, while improving procedural outcomes. Current clinical options suffer from a combination of high cost, poor material properties, and biocompatibility issues. Utilizing a technique called solution blow spinning, polymer fibers can be deposited directly onto any surface. Solution blow spinning has the potential to be a powerful tool in surgery and in biomaterials fabrication. The overall objective of this proposal is to investigate the use of solution blow spun polymer blends of poly(ethylene glycol) (PEG) and poly(lactic-co-glycolic acid) (PLGA) for use as a surgical sealant in an intestinal anastomosis model. The hypothesis for this research is: solution blow spun PLGA/PEG blends will have advantages over existing sealant strategies, leading to better surgical procedure outcomes. This proposal has been designed to utilize the complementary expertise and facilities at the University of Maryland's Functional Macromolecular Laboratory (UMD) and the Sheikh Zayed Institute for Pediatric Surgical Innovation at Children's National. The Specific Aims of the proposed research are: 1. Adhesive optimization, characterization, and in vitro assessment 2. Polymer functionalization for improved adhesion and in vitro characterization 3. In vivo pre- clinical efficacy and biocompatibility of blow spun polymer sealants in acute and chronic pre-clinical models. This work aims to correlate material properties and deposition conditions to in vitro and in vivo efficacy, and will lead to greater insight into the development of effective surgical materials. Ultimately, successful development of the direct deposition of polymer fiber constructs using solution blow spinning onto any surface could lead to clinically translatable approaches in a diverse variety of surgical applications and in biomaterials fabrication.