Breaking-up sitting to offset sedentary behaviors-induced metabolic alterations

打破久坐以抵消久坐行为引起的代谢变化

• 批准号: 9546666
• 负责人: Audrey Bergouignan
• 金额: $ 23.77万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9546666
• 关键词: Adult; Adverse effects; Advisory Committees; Award; Biochemistry; Biological; Biometry; Cardiovascular Diseases; Cells; Clinical Research; Collaborations; Complement; Data; Development Plans; Dietary Fats; Ensure; Environment; Exercise; Functional disorder; Genes; Glucose; Goals; Government; Health; Health Benefit; Individual; Insulin; Interruption; Intervention; Knowledge; Life; Lipids; Mentors; Metabolic; Metabolism; Mitochondria; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Outcome; Overweight; Phase; Physiology; Plasma; Population Study; Populations at Risk; Proteins; Public Health; Recommendation; Research; Scientist; Skeletal Muscle; Testing; Time; Tissues; Training; cardiometabolism; career development; combat; design; evidence base; exercise training; experience; improved; indexing; innovation; insulin sensitivity; moderate-to-vigorous physical activity; mortality; multidisciplinary; oxidation; post-doctoral training; programs; research study; sedentary; sedentary lifestyle; trafficking

This proposal outlines a career development plan to help Dr. Audrey Bergouignan complete her postdoctoral training and establish an independent research program focused on the physiopathology of sedentary behaviors. Her training will be conducted in a multidisciplinary group of scientists with research expertise in obesity, metabolism, whole-body and mitochondrial physiology, biochemistry and biostatistics. Her mentor, Dr. Daniel Bessesen, has an extensive experience with trainees that will be complemented by those in her advisory team, Drs Reusch, Murphy, Melanson and Pan. Collectively, this team will provide an outstanding training environment that will allow her to fill critical gaps in her toolbox needed to use an integrative approach from whole-body to tissue, cell, protein and gene levels to study mechanisms underlying the causal relationship between sedentary behaviors and adverse metabolic health outcomes. This award will also solidify her knowledge in biostatistics and ability to conduct clinical research studies. During the training phase of this award, she will strengthen her scholarly activities, establish important collaborations, and acquire critical data that will ensure her successful transition to independence. Rational: There is emerging evidence from population studies that time spent in sedentary behaviors is associated with all-cause mortality including obesity, type 2 diabetes, and cardiovascular diseases. Importantly, these associations are independent of time spent in exercise. Frequent interruptions to sedentary time are however beneficially associated with metabolic health outcomes, even in individuals who exercise regularly. Our hypothesis is that breaking-up prolonged sitting improves metabolic health. Our goal is to use integrative approach to understand the biological mechanisms that underlie associations of frequent interruptions of prolonged sitting with improved metabolic health outcomes. Design: A 24-hr study will first determine in overweight adults the effects of breaking-up prolonged sitting on whole-body substrate use and postprandial glucose and insulin concentrations, two core components of metabolic health. The first study will provide the applicant with training experiences and preliminary data to perform the more definitive study proposed in the R00 phase. The effects of 4 weeks of microbursts of activity will be compared with traditional caloric equivalent exercise training on plasma cardiometabolic markers, index of metabolic health. The underlying mechanisms, i.e. whole body substrate use, dietary fat oxidation and trafficking in skeletal muscle lipid fractions and mitochondrial function will be further delineated in association with insulin sensitivity. Relevance: We believe that the proposed studies will provide an initial evidence base for the health benefits of breaking up prolonged sitting with short bursts of activity. This innovative strategy may be more effective at combating the adverse effects of sedentary behaviors than more traditional approaches.

这个建议概述了一个职业发展计划，以帮助奥黛丽·伯居尼昂博士完成她的职业生涯