Core C: Biochemical and Molecular Core

项目摘要-核心C 生物化学和分子生物学核心（核心C）的主要目的是集中-两者都是比喻性的 和物理-支持的技术，是共同的大多数个别项目与此 计划项目补助金申请。因此，这个核心是研究人员的重要资源。 应用程序，个人在他们的实验室，是这个计划项目的重要组成部分。核心将 为项目调查人员提供技术支持、教学、特定关键设备的维护， 为常用的分子、生物化学和HPLC技术集中供应资源。我们还 开发了一个通过PPG网站以电子方式保存的方法/方案库， 提供给所有参与者。最后，我们继续开发新的方法， investigators.有两（2）个不同的科学焦点的核心。 中心一：第一个中心是支持基本的分子分析，包括实时PCR，Western分析， 免疫沉淀和免疫组织化学和细胞化学。该中心的一个具体重点是脂肪的分馏 转化为脂肪细胞和基质血管组分（SVF）。第一个核心中心将负责提供 进行所有这些测定的技术帮助，支持使用中心设备和一般 用品.核心D直接连接到核心C，因为它提供了用于评估 在Core C中生成的数据。 中心II：第二个中心是支持使用高压液体进行基于生物化学的分析 通过高效液相色谱法（HPLC）和ROS的生物化学测量。HPLC是这一核心的真实的优势， 研究者测量对PPG重要的各种物质（例如NE和代谢物）。

Project Summary – Core C The primary purpose of the Biochemical and Molecular Biology Core (Core C) is to centralize—both figuratively and physically- support for techniques that are common to a majority of the individual projects with this Program Project Grant application. This core is thus an important resource for the investigators of this application, the individuals in their laboratories and is an essential part of this Program Project. This core will provide project investigators with technical support, teaching, upkeep of particular key pieces of equipment and centralize supply sources for commonly-used molecular, biochemical and HPLC techniques. We have also developed a repository of methods/protocols kept electronically through the PPG website, protocols that are available to all participants in the program. Finally, we continue to develop new methods that benefit our investigators. There are two (2) distinct scientific foci of this core. Center I: The first center is support for basic molecular analyses, including real-time PCR, Western analyses, immunoprecipitation and immunohisto- and cytochemistry. A specific focus of this Center is fractionation of fat into adipose cells and stromal vascular fractions (SVF). This first core center will be responsible for providing technical help for performing all of these assays, support for use of central pieces of equipment and general supplies. Core D connects directly to Core C in that it provides the microscopic expertise for evaluation of the data produced within Core C. Center II: The second center is support for biochemical-based analyses using high pressure liquid chromatography (HPLC) and biochemical measurement of ROS. HPLC is a real strength of this core, enabling investigators to measure a variety of substances important to the PPG (e.g. NE and metabolites).