Protein Structure Laboratory

蛋白质结构实验室

• 批准号: 9315167
• 负责人: Scott W. Lovell
• 金额: $ 7.03万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9315167
• 关键词: Applications Grants; Centers of Research Excellence; Computer software; Consultations; Crystallization; Crystallography; Data Analyses; Data Collection; Databases; Deposition; Doctor of Philosophy; Faculty; Government; Grant; Growth; Industrialization; Institution; Kansas; Laboratories; Learning; Methods; Mission; Modernization; Phase; Postdoctoral Fellow; Preparation; Proteins; Publications; Research Assistant; Research Personnel; Roentgen Rays; Sampling; Services; Structure; Students; Synchrotrons; Time; Training; Universities; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; base; experience; experimental study; instrumentation; protein function; protein structure; screening; structural biology; three dimensional structure

The mission ofthe Protein Structure Laboratory (PSL) is to provide investigators with state-of-the-art instrumentation, facilities, and expertise for all aspects of protein crystallography. This includes protein crystallization. X-ray data collection, data analysis, structure solution and refinement, and structure analysis. Laboratory staff provides advice and consultation, training, access to facilities for experienced crystallographers, and a range of services from crystal growth to structure solution and refinement, and preparation of materials for publications and grant submissions. In addition, the PSL provides training to students, post-docs and faculty who wish to learn any or all aspects of protein structure determination. To facilitate this, modern crystallization methods, in-house or synchrotron X-ray diffraction data collection, and the most recent crystallographic software are utilized. The staff consists of one experienced full time Ph.D. crystallographer. Dr. Scott Lovell, who serves as the Director ofthe Laboratory, and a Research Assistant to assist with crystallization screening and sample preparation for X-ray diffraction experiments. The PSL is located in close proximity to other core service laboratories in the University of Kansas Structural Biology Center building. During Phase II, the PSL has collaborated with 36 investigators from academic, government and industrial institutions. This has resulted in more than 100 structures delivered, 64 deposited to the Protein Databank, 26 publications and 17 grant applications submitted in which the PSL staff were listed. The PSL will continue to expand the base of collaborators and provide high-level facilities, expertise and training during Phase III ofthe COBRE-PSF.

蛋白质结构实验室(PSL)的使命是为研究人员提供最先进的 蛋白质结晶学方方面面的仪器、设备和专业知识。这包括蛋白质 结晶。X射线数据收集、数据分析、结构解算和细化、结构分析。 实验室工作人员为有经验的人员提供建议和咨询、培训、使用设施 以及从晶体生长到结构解决方案和精炼的一系列服务，以及 为出版物和赠款申请准备材料。此外，PSL还提供培训，以 希望学习蛋白质结构测定的任何或所有方面的学生、博士后和教职员工。至 促进这一点，现代结晶方法，内部或同步辐射X射线衍射数据收集，以及 使用了最新的结晶学软件。工作人员由一名经验丰富的全日制博士组成。 结晶学家。斯科特·洛弗尔博士，实验室主任，研究助理 协助X-射线衍射实验的结晶筛选和样品制备。PSL是 位于堪萨斯大学结构生物学其他核心服务实验室附近 中心大楼。在第二阶段，PSL与36名来自学术界、 政府和工业机构。说，这导致了100多个结构物的交付 对于蛋白质数据库，提交了26份出版物和17份赠款申请，其中PSL工作人员 已列出。PSL将继续扩大合作伙伴的基础，并提供高水平的设施、专业知识 并在科布雷-PSF第三阶段期间进行培训。