Integrated Phosphor and Fluorescence Imager (Typhoon FLA9500)

集成荧光粉和荧光成像仪 (Typhoon FLA9500)

• 批准号: 9075819
• 负责人: Jack H Nunberg
• 金额: $ 13.81万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075819
• 关键词: Back; Biological Sciences; Biotechnology; Charge; Detection; Fees; Fluorescence; Funding; Gel; Healthcare; Image; Imagery; Lasers; Molecular Biology; Montana; Nucleic Acids; Proteins; Radiolabeled; Research Personnel; Research Project Grants; Resolution; Scanning; Signal Transduction; Staining method; Stains; Structure; United States National Institutes of Health; Universities; fluorescence imaging; instrument; quantitative imaging; radiotracer; research study

 DESCRIPTION (provided by applicant): This proposal seeks funding to purchase a Typhoon FLA9500 biomolecular imager (GE Healthcare Life Sciences) to replace a now-obsolete Fuji FLA3000G that has served our shared needs for 15 years. The Typhoon FLA9500 is a high-resolution high-sensitivity laser-scanning instrument with applications in phosphorimaging and fluorescence, chemifluorescence and colorimetric imaging. The current Fuji FLA3000G is the sole integrated biomolecular scanner on the University of Montana campus and is used by virtually all NIH-supported biomedical researchers for quantitative imaging in a broad range of routine molecular biology experiments: phosphorimaging of radiolabeled biomolecules, visualization of fluorescently stained proteins and nucleic acids in gels, and detection of chemifluorescent substrate in blots and arrays. The Typhoon FLA9500 includes standard blue, green and red lasers similar to those in the Fuji instrument and adds new capability for multiplexing two fluorescence signals. The replacement instrument will continue to be administered by the Montana Biotechnology Center using an established charge-back fee structure. Purchase of the Typhoon FLA9500 will provide biomedical researchers at the University of Montana continued access to high-quality phosphor and fluorescence imaging to support basic molecular biology applications in a wide range of research projects.

 描述（由申请人提供）：本提案寻求资金购买Typhoon FLA9500生物分子成像仪（GE Healthcare Life Sciences），以取代现已过时的Fuji FLA3000G，该成像仪已满足我们的共同需求15年。Typhoon FLA9500是一种高分辨率、高灵敏度的激光扫描仪器，可应用于磷光成像、荧光、化学荧光和比色成像。目前的富士FLA3000G是蒙大拿大学校园内唯一的集成生物分子扫描仪，几乎所有NIH支持的生物医学研究人员都使用它来进行广泛的常规分子生物学实验中的定量成像：放射性标记生物分子的磷光成像，凝胶中荧光染色蛋白质和核酸的可视化，以及印迹和阵列中化学荧光底物的检测。Typhoon FLA9500包括与富士仪器相似的标准蓝色、绿色和红色激光器，并增加了多路复用两个荧光信号的新功能。替代仪器将继续由蒙大拿生物技术中心使用既定的收费结构管理。购买Typhoon FLA9500将为蒙大拿大学的生物医学研究人员提供持续获得高质量荧光粉和荧光成像的机会，以支持广泛研究项目中的基础分子生物学应用。