Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis

全血多重病原体检测技术在脓毒症快速诊断中的应用 摘要 败血症，一种对感染的不良自身免疫反应，通常会导致危及生命的 并发症，导致在美国医院的任何疾病的死亡率和治疗费用最高。 在这项提案中，我们提出了一种新的微芯片，以弥合现有的差距，在病原体 检测（灵敏度差，处理时间长）。 我们的策略在拟议的工作是进行皮升体积RT-LAMP反应的干燥 血液使用我们内部开发的双相反应，以实现高度敏感的病原体 计数（1-3 CFU/mL）。 该微孔阵列平台的实现潜在地克服了微孔阵列平台的局限性。 目前血培养的灵敏度和反应时间。我们预计，该设备将 大大有助于脓毒症的快速诊断。作为最终目标，我们预计， 该平台将取代使用血培养进行病原体检测。

Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis Abstract Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. In this proposal we propose a novel microchip to bridge the existing gaps in the pathogen detection (poor sensitivity and long processing time). Our strategy in the proposed work is performing picolitre volume RT-LAMP reactions on dried blood using our in-house developed bi-phasic reaction to achieve a highly sensitive pathogen count (1-3 CFU/mL). The realization of this microwell array platform potentially overcomes the limitations in sensitivity and time of response of the current blood cultures. We expect that this device will greatly contribute to the faster rapid diagnosis of sepsis. As a final goal, we anticipate that this platform will replace the use of blood culture for pathogen detection.