Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis

全血多重病原体检测可快速检测脓毒症

• 批准号: 9809870
• 负责人: Rashid Bashir
• 金额: $ 23.01万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-06 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9809870
• 关键词: Acquired Immunodeficiency Syndrome; Autoimmune Responses; Biological; Blood; Blood Volume; Blood specimen; Cessation of life; Chlamydophila pneumoniae; Clinic; Clinical; Detection; Devices; Diagnosis; Diagnostic; Diagnostic tests; Disease; Escherichia coli; Functional disorder; Generations; Goals; Healthcare Systems; Histologic; Hospitals; Hour; Infection; Inflammatory; Inflammatory Response; Knowledge; Life; Liquid substance; Literature; Malignant neoplasm of prostate; Mediating; Microbiology; Morbidity - disease rate; Nucleic Acids; Organ; Pathogen detection; Patients; Pharmaceutical Preparations; Phase; Preparation; Process; Protocols documentation; Publishing; RNA-Directed DNA Polymerase; Reaction; Reaction Time; Reagent; Reporting; Resolution; Sampling; Screening procedure; Sepsis; Solid; Staphylococcus aureus; Survival Rate; Syndrome; Systemic Inflammatory Response Syndrome; Techniques; Technology; Time; Tissues; Translating; Treatment Cost; Whole Blood; Work; anticancer research; antimicrobial; base; cost; digital; genetic information; improved; interest; malignant breast neoplasm; microchip; milliliter; mortality; multiplex detection; novel; pathogen; rapid detection; rapid diagnosis; scale up; septic patients; tool

Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis Abstract Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. In this proposal we propose a novel microchip to bridge the existing gaps in the pathogen detection (poor sensitivity and long processing time). Our strategy in the proposed work is performing picolitre volume RT-LAMP reactions on dried blood using our in-house developed bi-phasic reaction to achieve a highly sensitive pathogen count (1-3 CFU/mL). The realization of this microwell array platform potentially overcomes the limitations in sensitivity and time of response of the current blood cultures. We expect that this device will greatly contribute to the faster rapid diagnosis of sepsis. As a final goal, we anticipate that this platform will replace the use of blood culture for pathogen detection.

全血多重病原体检测技术在脓毒症快速诊断中的应用 摘要 败血症，一种对感染的不良自身免疫反应，通常会导致危及生命的 并发症，导致在美国医院的任何疾病的死亡率和治疗费用最高。 在这项提案中，我们提出了一种新的微芯片，以弥合现有的差距，在病原体 检测（灵敏度差，处理时间长）。 我们的策略在拟议的工作是进行皮升体积RT-LAMP反应的干燥 血液使用我们内部开发的双相反应，以实现高度敏感的病原体 计数（1-3 CFU/mL）。 该微孔阵列平台的实现潜在地克服了微孔阵列平台的局限性。 目前血培养的灵敏度和反应时间。我们预计，该设备将 大大有助于脓毒症的快速诊断。作为最终目标，我们预计， 该平台将取代使用血培养进行病原体检测。