Microfabricated instrumentation to measure sphingolipid signaling in human acute myeloid leukemia

用于测量人类急性髓系白血病中鞘脂信号传导的微型仪器

基本信息

  • 批准号:
    9809343
  • 负责人:
  • 金额:
    $ 58.68万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-05-07 至 2019-12-31
  • 项目状态:
    已结题

项目摘要

An innovative platform to measure the activity of the sphingolipid pathway in single cells from primary, human, acute myeloid leukemia (AML) will be developed. A multidisciplinary group (chemist, bioengineer, oncologist and computational scientist) with a history of successful collaborations will pursue the development of engineered microfluidic instrumentation and supporting hardware using medically relevant probes to answer fundamental questions regarding heterogeneity in single AML cells. Fluorescent probes to track simultaneously the three major pathways comprising the ceramide-sphingosine axis in AML cells will be developed so that a detailed understanding of sphingolipid signaling in the tumor cells is achieved. Electrophoretic separations within a microfabricated device will be optimized for the single-cell measurements as a component of the work flow. Automation and integration will greatly increase throughput to yield a microdevice which is compatible with common clinical workflows. A powerful attribute of the proposal is that these measurements will be performed on single cells from primary samples and will avoid the confounding aspects of population- averaged data yielded by bulk cell assays. Furthermore, by simultaneously tracking all arms of the sphingolipid pathway, we will identify the strategies that AML cells use to dynamically reprogram their growth-promoting pathways via sphingolipid signaling during drug treatment. The proposed microfabricated devices will in the future provide key information concerning the best treatment option(s) for patients as well yielding an assessment of treatment efficacy to contribute fundamental data to the emerging field of precision medicine.
一个创新的平台来测量鞘脂途径的活性在单细胞从

项目成果

期刊论文数量(0)
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会议论文数量(0)
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Nancy L. Allbritton其他文献

Choosing one from the many: selection and sorting strategies for single adherent cells
  • DOI:
    10.1007/s00216-006-0612-1
  • 发表时间:
    2006-07-18
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Christopher E. Sims;Mark Bachman;G. P. Li;Nancy L. Allbritton
  • 通讯作者:
    Nancy L. Allbritton
Erratum to: Trapping cells on a stretchable microwell array for single-cell analysis
  • DOI:
    10.1007/s00216-012-6266-2
  • 发表时间:
    2012-07-21
  • 期刊:
  • 影响因子:
    3.800
  • 作者:
    Yuli Wang;Pavak Shah;Colleen Phillips;Christopher E. Sims;Nancy L. Allbritton
  • 通讯作者:
    Nancy L. Allbritton
Measuring the Enzymatic Activity of Clinically Important Proteins in Single Cells
  • DOI:
    10.1016/j.bpj.2010.12.1401
  • 发表时间:
    2011-02-02
  • 期刊:
  • 影响因子:
  • 作者:
    Christopher E. Sims;Nancy L. Allbritton;Dechen Jiang;Shan Yang;Angie Proctor;Ryan Phillips
  • 通讯作者:
    Ryan Phillips
Imaging 3D cell cultures with optical microscopy
用光学显微镜对三维细胞培养进行成像
  • DOI:
    10.1038/s41592-025-02647-w
  • 发表时间:
    2025-04-17
  • 期刊:
  • 影响因子:
    32.100
  • 作者:
    Huai-Ching Hsieh;Qinghua Han;David Brenes;Kevin W. Bishop;Rui Wang;Yuli Wang;Chetan Poudel;Adam K. Glaser;Benjamin S. Freedman;Joshua C. Vaughan;Nancy L. Allbritton;Jonathan T. C. Liu
  • 通讯作者:
    Jonathan T. C. Liu
emClostridioides difficile/em-mucus interactions encompass shifts in gene expression, metabolism, and biofilm formation
艰难梭菌与黏液的相互作用包括基因表达、代谢和生物膜形成的变化
  • DOI:
    10.1128/msphere.00081-24
  • 发表时间:
    2024-05-14
  • 期刊:
  • 影响因子:
    3.100
  • 作者:
    Kathleen L. Furtado;Lucas Plott;Matthew Markovetz;Deborah Powers;Hao Wang;David B. Hill;Jason Papin;Nancy L. Allbritton;Rita Tamayo;Craig D. Ellermeier
  • 通讯作者:
    Craig D. Ellermeier

Nancy L. Allbritton的其他文献

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{{ truncateString('Nancy L. Allbritton', 18)}}的其他基金

Development of a microphysiologic system to assay the interaction of the human colonic epithelium on Clostridium difficile
开发微生理系统来测定人结肠上皮对艰难梭菌的相互作用
  • 批准号:
    10321276
  • 财政年份:
    2020
  • 资助金额:
    $ 58.68万
  • 项目类别:
Development of a microphysiologic system to assay the interaction of the human colonic epithelium on Clostridium difficile
开发微生理系统来测定人结肠上皮对艰难梭菌的相互作用
  • 批准号:
    10539253
  • 财政年份:
    2020
  • 资助金额:
    $ 58.68万
  • 项目类别:
Development of a microphysiologic system to assay the interaction of the human colonic epithelium on Clostridium difficile
开发微生理系统来测定人结肠上皮对艰难梭菌的相互作用
  • 批准号:
    9884925
  • 财政年份:
    2020
  • 资助金额:
    $ 58.68万
  • 项目类别:
MICROFABRICATED INSTRUMENTATION TO MEASURE SPHINGOLIPID SIGNALING IN HUMAN ACUTE MYELOID LEUKEMIA
用于测量人类急性髓系白血病中鞘脂信号传导的微型仪器
  • 批准号:
    10667508
  • 财政年份:
    2019
  • 资助金额:
    $ 58.68万
  • 项目类别:
MICROFABRICATED INSTRUMENTATION TO MEASURE SPHINGOLIPID SIGNALING IN HUMAN ACUTE MYELOID LEUKEMIA
用于测量人类急性髓系白血病中鞘脂信号传导的微型仪器
  • 批准号:
    9926834
  • 财政年份:
    2019
  • 资助金额:
    $ 58.68万
  • 项目类别:
PROFILING SIGNALING ACTIVITY AND GENE EXPRESSION IN SINGLE, PANCREATIC ADENOCARCINOMA CELLS USING CE-RNA-SEQ
使用 CE-RNA-SEQ 对单个胰腺腺癌细胞中的信号传导活性和基因表达进行分析
  • 批准号:
    10373116
  • 财政年份:
    2018
  • 资助金额:
    $ 58.68万
  • 项目类别:
PROFILING SIGNALING ACTIVITY AND GENE EXPRESSION IN SINGLE, PANCREATIC ADENOCARCINOMA CELLS USING CE-RNA-SEQ
使用 CE-RNA-SEQ 对单个胰腺腺癌细胞中的信号传导活性和基因表达进行分析
  • 批准号:
    10115487
  • 财政年份:
    2018
  • 资助金额:
    $ 58.68万
  • 项目类别:
PROFILING SIGNALING ACTIVITY AND GENE EXPRESSION IN SINGLE, PANCREATIC ADENOCARCINOMA CELLS USING CE-RNA-SEQ
使用 CE-RNA-SEQ 分析单个胰腺腺癌细胞中的信号传导活性和基因表达
  • 批准号:
    10200700
  • 财政年份:
    2018
  • 资助金额:
    $ 58.68万
  • 项目类别:
Development of Human Intestinal Simulacra
人体肠道模拟物的开发
  • 批准号:
    9767231
  • 财政年份:
    2015
  • 资助金额:
    $ 58.68万
  • 项目类别:
Development of Human Intestinal Simulacra
人体肠道模拟物的开发
  • 批准号:
    8948275
  • 财政年份:
    2015
  • 资助金额:
    $ 58.68万
  • 项目类别:

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Computing analysis of leukemic stem cell dynamics in acute myelocytic leukemia
急性粒细胞白血病白血病干细胞动力学的计算分析
  • 批准号:
    19K08356
  • 财政年份:
    2019
  • 资助金额:
    $ 58.68万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Generation of immunotoxins with super-targeting mAb in the acute myelocytic leukemia
在急性髓细胞白血病中使用超靶向单克隆抗体产生免疫毒素
  • 批准号:
    23501309
  • 财政年份:
    2011
  • 资助金额:
    $ 58.68万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DETERMINANTS OF RESPONSE OF ACUTE MYELOCYTIC LEUKEMIA
急性粒细胞白血病反应的决定因素
  • 批准号:
    3556971
  • 财政年份:
    1980
  • 资助金额:
    $ 58.68万
  • 项目类别:
DETERMINANTS OF RESPONSE OF ACUTE MYELOCYTIC LEUKEMIA
急性粒细胞白血病反应的决定因素
  • 批准号:
    3556968
  • 财政年份:
    1980
  • 资助金额:
    $ 58.68万
  • 项目类别:
ERADICATION OF ACUTE MYELOCYTIC LEUKEMIA CELLS BY MAB THERAPY
通过 MAB 疗法根除急性粒细胞白血病细胞
  • 批准号:
    3889304
  • 财政年份:
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    $ 58.68万
  • 项目类别:
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