IGF::OT::IGF SBIR Topic 355: An Organoid Avatar Biobank for Cancer Disparities. To establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient populatio

IGF::OT::IGF SBIR 主题 355：癌症差异的类器官化身生物库。

• 批准号: 9574776
• 负责人: Carolyn Bannister
• 金额: $ 30万
• 依托单位: DELPHI GENOMICS, LLC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-19 至 2018-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9574776
• 关键词: 3-Dimensional; African; African American; American; Colon; Colon Carcinoma; Colonic Neoplasms; Colorectal Cancer; Dependence; EGF gene; European; FDA approved; Gene Mutation; Measures; Mutation; Oncogenes; Operative Surgical Procedures; Organoids; Patients; Pharmaceutical Preparations; Preclinical Drug Evaluation; Small Business Innovation Research Grant; Somatic Mutation; Specimen; Stem cells; TGFB1 gene; TP53 gene; Work; biobank; cancer health disparity; mutant; oncology; patient population; tumor

This project seeks to establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient population that is approximately 40% African American. We will make use of discarded, de-identified surgical specimens to establish 3-dimensional cultures of tumor and matched normal colon stem cells (organoids). Organoid avatars will be characterized by 1) WNT, EGF, and TGFB (in) dependence 2) presence of somatic mutations in colon cancer genes, 3) percent African ancestry, 4) measure IC50s for select drugs from the Approved Oncology Drugs panel, and 5) create isogenic tumor organoids with select colon cancer driver gene mutations suitable for drug screening efforts. This work will create organoid lines, which will be used to advance our understanding of basic mechanisms of cancer health disparities among African Americans.

该项目旨在建立一个结直肠癌类器官化身生物库，该生物库来自种族和遗传多样性的患者人群，约40%为非洲裔美国人。我们将利用废弃的、去识别的手术标本来建立肿瘤和匹配的正常结肠干细胞（类器官）的三维培养物。类器官化身的特征在于：1）WNT、EGF和TGFB（不）依赖性; 2）结肠癌基因中存在体细胞突变; 3）非洲血统百分比; 4）测量来自批准的肿瘤药物小组的选定药物的IC 50; 5）创建具有适合药物筛选工作的选定结肠癌驱动基因突变的等基因肿瘤类器官。这项工作将创建类器官线，这将用于促进我们对非裔美国人癌症健康差异的基本机制的理解。