IGF::OT::IGF SBIR Topic 355: An Organoid Avatar Biobank for Cancer Disparities. To establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient populatio

该项目旨在从种族和基因多样化的患者群体中建立一个结直肠癌类器官化身生物库，其中约40%为非洲裔美国人。我们将利用丢弃的、去识别的手术标本建立肿瘤和匹配的正常结肠干细胞（类器官）的三维培养。类器官化身将具有以下特征：1)WNT、EGF和TGFB依赖性；2)结肠癌基因中存在体细胞突变；3)非洲血统的百分比；4)从已批准的肿瘤药物小组中选择药物测量ic50； 5)创建具有适合药物筛选工作的结肠癌驱动基因突变的等基因肿瘤类器官。这项工作将创造出类器官细胞系，用于促进我们对非裔美国人癌症健康差异的基本机制的理解。

This project seeks to establish a colorectal cancer organoid avatar bio-bank from an ethnically and genetically diverse patient population that is approximately 40% African American. We will make use of discarded, de-identified surgical specimens to establish 3-dimensional cultures of tumor and matched normal colon stem cells (organoids). Organoid avatars will be characterized by 1) WNT, EGF, and TGFB (in) dependence 2) presence of somatic mutations in colon cancer genes, 3) percent African ancestry, 4) measure IC50s for select drugs from the Approved Oncology Drugs panel, and 5) create isogenic tumor organoids with select colon cancer driver gene mutations suitable for drug screening efforts. This work will create organoid lines, which will be used to advance our understanding of basic mechanisms of cancer health disparities among African Americans.