Magnetically directed single cell transcriptome analysis in HIV latency

HIV潜伏期的磁定向单细胞转录组分析

• 批准号: 9538888
• 负责人: DAVID MARTIN MURDOCH
• 金额: $ 4.82万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-20 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9538888
• 关键词: Address; Antigen-Antibody Complex; Biological; Biological Assay; Biological Models; Biomedical Engineering; Biosensor; Cell Communication; Cell Culture Techniques; Cell Line; Cell Separation; Cell Survival; Cell model; Cells; Clonal Expansion; Collaborations; Complex; Computer software; Computers; Detection; Development; Devices; Disease; Electromagnetics; Electrons; Engineering; Epigenetic Process; Event; Exposure to; Flow Cytometry; Gene Expression Profiling; Genetic Transcription; Genotype; Goals; HDAC4 gene; HIV; Histone Deacetylase Inhibitor; Immune; Immune response; Immune system; Immunologics; Immunology; Immunotherapeutic agent; Immunotherapy; In Vitro; Incubators; Individual; Kinetics; Knowledge; Lab-On-A-Chips; Label; Laboratories; Longitudinal Studies; Magnetism; Measures; Methods; Microfluidics; Minority; Modeling; Morphology; Movement; National Institute of Allergy and Infectious Disease; Pharmacologic Substance; Pharmacology; Phenotype; Play; Reaction; Regulation; Research; Research Personnel; Research Project Grants; Retrieval; Reverse Transcriptase Polymerase Chain Reaction; Role; Scientist; Shock; Sorting - Cell Movement; Stimulus; Surface; System; Systems Biology; T-Lymphocyte; Techniques; Testing; Time; Viral Proteins; Viral reservoir; Virion; Virus-Cell Membrane Interaction; Work; cancer immunotherapy; chromatin immunoprecipitation; design; drug candidate; experimental study; human disease; in vivo; interdisciplinary collaboration; interest; killings; mathematical model; novel; novel strategies; novel therapeutics; programs; public health relevance; single cell analysis; tool; transcriptome

 DESCRIPTION (provided by applicant): This project leverages new collaborations between biomedical and engineering investigators to develop new methods for the sorting and isolation of immune cells, where the precise trajectory of individual cells are controlled and sorted on a chip analogous to the way electrons are controlled inside computer circuits. This set of tools allows for fundamentally new methods to study phenotype, genotype, and the morphology of single or pairs of single cells, over extended periods of time with precision that is unparalleled by existing techniques, such as flow cytometry and flow cell sorting. The overall stated aims of this research are to develop the engineering platform and demonstrate a biological application for a lab-on-a-chip device that can analyze thousands of single cells over long durations, exposure to multiple stimuli, and enable the extraction of individual, high-value, cells for furthe immunological analyses (RT-PCR, clonal expansion, etc.). We aim to 1) design and fabricate a lab-on-chip assay optimized for biological applications, 2) optimize the assay for biological applications, and 3) use this novel assay to study early cellular events in the disruption of HIV latency.