Magnetically directed single cell transcriptome analysis in HIV latency

HIV潜伏期的磁定向单细胞转录组分析

基本信息

批准号：
9064352
负责人：
DAVID MARTIN MURDOCH
金额：
$ 51.09万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-20 至 2021-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9064352
关键词：
Address Biological Biological Assay Biological Models Biomedical Engineering Biosensor Cell Communication Cell Culture Techniques Cell Line Cell Separation Cell Survival Cells Clonal Expansion Collaborations Complex Computer software Computers Detection Development Devices Disease Electromagnetics Electrons Engineering Epigenetic Process Event Exposure to Flow Cytometry Gene Expression Profiling Genetic Transcription Genotype Goals HIV Histone Deacetylase Inhibitor Immune Immune response Immune system Immunology Immunotherapeutic agent Immunotherapy In Vitro Incubators Individual Kinetics Knowledge Label Laboratories Longitudinal Studies Magnetism Measures Methods Microfluidics Minority Modeling Morphology Movement National Institute of Allergy and Infectious Disease Pharmacologic Substance Pharmacology Pharmacotherapy Phenotype Play Reaction Regulation Research Research Personnel Research Project Grants Retrieval Reverse Transcriptase Polymerase Chain Reaction Role Scientist Shock Sorting - Cell Movement Stimulus Surface System Systems Biology T-Lymphocyte Techniques Testing Time Transcriptional Activation Viral Proteins Viral reservoir Virion Virus-Cell Membrane Interaction Work cancer immunotherapy chromatin immunoprecipitation design dimensional analysis drug candidate human disease in vivo interdisciplinary collaboration interest killings mathematical model micro-total analysis system novel novel strategies novel therapeutics programs public health relevance research study tool transcriptome

项目摘要

DESCRIPTION (provided by applicant): This project leverages new collaborations between biomedical and engineering investigators to develop new methods for the sorting and isolation of immune cells, where the precise trajectory of individual cells are controlled and sorted on a chip analogous to the way electrons are controlled inside computer circuits. This set of tools allows for fundamentally new methods to study phenotype, genotype, and the morphology of single or pairs of single cells, over extended periods of time with precision that is unparalleled by existing techniques, such as flow cytometry and flow cell sorting. The overall stated aims of this research are to develop the engineering platform and demonstrate a biological application for a lab-on-a-chip device that can analyze thousands of single cells over long durations, exposure to multiple stimuli, and enable the extraction of individual, high-value, cells for furthe immunological analyses (RT-PCR, clonal expansion, etc.). We aim to 1) design and fabricate a lab-on-chip assay optimized for biological applications, 2) optimize the assay for biological applications, and 3) use this novel assay to study early cellular events in the disruption of HIV latency.

描述（由应用程序提供）：该项目利用生物医学和工程研究人员之间的新合作来开发用于分类和隔离免疫电池的新方法，在这种方法中，单个单元格的精确轨迹受到控制和分类的芯片类似，类似于电脑内部电脑内部电机内部的电子设备。这组工具允许从根本上进行新的方法研究表型，基因型和单个单元或成对的单个单元的形态，在长时间的时间内，精确的精确度与现有技术（例如流式细胞仪和流动细胞分类）无与伦比。这项研究的总体说明的目的是开发工程平台，并展示了用于实验室芯片设备的生物学应用，该设备可以在长时间长时间内分析数千个单个单元，暴露于多个刺激中，并可以提取个体，高价值的细胞，以促进免疫学分析（RT-PCR，Clonal扩展等）。我们的目的是1）设计和制造针对生物应用优化的实验室芯片测定法，2）优化生物学应用测定法，3）使用这种新颖的测定法研究HIV潜伏期破坏的早期细胞事件。