Molecular Genetics of Evolutionary Innovations

项目总结 “进化创新”指的是全新特征的起源，而不是 现有的特征。尽管这样的新奇事物相对较少，但生活的所有复杂性和多样性 最终由以嵌套模式发生的进化创新塑造，每一项创新 依赖于许多早期的新奇事物。尽管它们对从地质到地质的一切事物都至关重要 支持地球上所有生命的营养循环与人类区别于我们最亲密的人的感觉 亲属们，人们对进化创新的分子机制的了解远远少于对进化的了解 已有的特征。这在生物组织的所有层面上都是如此，从单分子到大多数 动物形态和功能的复杂特征。我们了解现有器官和细胞的进化 类型比新类型的起源更好；基因表达的数量差异已在 比新的调控途径的起源要详细得多；关于 关于现有基因比新基因的起源等等.正是我们知识中的这种鸿沟促使我们 我们的工作。 为了实现对进化创新的全面理解，有必要将 生物组织各个层面的新颖性：从基因组中的新功能元素到新基因 通向新的形态结构。我们实验室的研究将向三个方向发展，使用 果蝇模型系统。首先，我们将确定负责新的起源的分子机制 最近在果蝇体内进化的形态结构。我们将鉴定DNA序列， 引起了表型创新，并重建了细胞分化途径，而不是翻译这些 变成了新的形态。第二，我们将定性地研究基因组机制 重建不同器官和细胞类型的基因表达谱，并量化相对 每种类型的基因组变化对每个组织中表达的基因周转的贡献。我们会 测试控制基因表达的调控电路是否主要通过掺入 单个基因，或通过招募更大的遗传模块。第三，我们将确定分子变化 负责控制非功能性基因表达的新调控元件的来源 祖先序列。通过专注于在一个自然种群中进化的新的监管元素 单个物种，我们将重建一系列突变，这些突变在细胞内产生新的功能元件 基因组，并阐明这些突变对基因调控的影响。加在一起，这些方法将 促进对进化创新的深入机械论理解。

Project summary “Evolutionary innovation” refers to the origin of entirely new traits, as opposed to the modification of existing traits. Although such novelties are relatively rare, all the complexity and diversity of life has ultimately been shaped by evolutionary innovations that occurred in a nested pattern, with every innovation dependent on many earlier novelties. Despite their critical importance for everything from geological nutrient cycles that support all life on Earth to human sentience that distinguishes us from our closest relatives, the molecular mechanisms of evolutionary innovations are understood far less than the evolution of existing traits. This is true at all levels of biological organization, from single molecules to the most complex features of animal form and function. We understand the evolution of existing organs and cell types better than the origin of new ones; quantitative variation in gene expression has been explored in much greater detail than the origin of novel regulatory pathways; much more is known about the evolution of existing genes than about the origin of new genes, and so on. It is this gap in our knowledge that motivates our work. To achieve a comprehensive understanding of evolutionary innovations, it is necessary to connect novelties at all levels of biological organization: from new functional elements in the genome, to new genetic pathways, to new morphological structures. Research in our lab will advance in three directions, using the Drosophila model system. First, we will identify the molecular mechanisms responsible for the origin of new morphological structures that evolved recently within Drosophila. We will identify the DNA sequences that gave rise to phenotypic innovations, and reconstruct the cell differentiation pathways than translate these changes into novel morphologies. Second, we will examine the genomic mechanisms that qualitatively remodel the gene expression profiles of different organs and cell types, and quantify the relative contributions of each type of genomic change to the turnover of genes expressed in each tissue. We will test whether the regulatory circuits that control gene expression evolve predominantly by incorporating individual genes, or by recruitment of larger genetic modules. Third, we will identify the molecular changes responsible for the origin of new regulatory elements that control gene expression from non-functional ancestral sequences. By focusing on novel regulatory elements that evolved within natural populations of a single species, we will reconstruct the series of mutations that create new functional elements in the genome, and elucidate the impact of these mutations on gene regulation. Together, these approaches will promote a deep mechanistic understanding of evolutionary innovations.