Core D: Thymic and peripheral Aspects of T cell Aging and Rejuvenation

核心 D：T 细胞衰老和再生的胸腺和外周方面

• 批准号: 9353555
• 负责人: Megan J Smithey
• 金额: $ 25.45万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9353555
• 关键词: Aging; Animals; Antibodies; Antibody Formation; Arizona; B-Lymphocytes; Biological Assay; Biological Models; CD4 Positive T Lymphocytes; CD8-Positive T-Lymphocytes; Cell Aging; Cell Maintenance; Cell physiology; Cellularity; Communicable Diseases; Competence; Cost Savings; Data; Data Analyses; Data Collection; Elderly; Emigrant; Ensure; Failure; Female; Generations; Goals; Human; Human Resources; Immune; Immune response; Immune system; Immunity; Immunology procedure; Individual; Intervention; Knowledge; Laboratories; Maintenance; Measures; Mediating; Modeling; Monitor; Morbidity - disease rate; Mus; Neuraxis; Output; Pathway interactions; Peripheral; Predisposition; Procedures; Program Research Project Grants; Protocols documentation; Quality Control; Rejuvenation; Research; Research Personnel; Resolution; Services; Specimen Handling; Spleen; T-Lymphocyte; T-Lymphocyte Epitopes; Testing; Thymus Gland; Time; TimeLine; Tissue Sample; Training; Translating; Universities; Virus Diseases; West Nile viral infection; West Nile virus; adaptive immunity; aged; animal resource; arm; biosafety level 3 facility; experimental study; functional improvement; genetic approach; immune function; improved; in vivo; lymph nodes; male; member; mortality; mouse model; mutant mouse model; pathogen; peripheral blood; pre-clinical; programs; response; sample collection; transcription factor

Core D- Abstract Increased susceptibility to infectious disease is the most prominent repercussion of the aging immune system. In addition to generating basic knowledge, Projects 1-4 of the “Thymic and peripheral aspects of T cell aging and rejuvenation” Program Project Grant (Program) will provide an array of potential rejuvenation strategies. The Immunological Response & Rejuvenation Strategy Monitoring Core (Core D), located at the University of Arizona, will provide the final experimental pipeline for testing those rejuvenation strategies. Specifically, the global objective of Core D is to assess thymic export and peripheral maintenance of naïve T cells following T cell rejuvenation in aged mice, and determine whether such interventions can improve adaptive and protective immunity against viral infection in old mice. The Core will provide dedicated personnel with the appropriate infectious disease expertise to ensure consistency and quality control in the execution of study protocols, application of experimental procedures, and animal observations and data collection necessary to meet the Program's major research objectives. This testing pipeline will not only separate candidate interventions by their efficacy, but will also identify potential stages at which some rejuvenation strategies may fail to translate into improved immune function. The use of rigorous in vivo analysis to track recent thymic emigrants, and immunity in response to West Nile virus, a pathogen that shows disproportionally high morbidity and mortality within the elderly, will provide this Program and its Projects stringent testing of a full spectrum of peripheral immune function and protective immunity following T cell rejuvenation in aged mice. Thereby, the Core will deliver rigorous preclinical data, that, together with the Human-Mouse Timeline (generated by Projects 1-4 and other Cores) will enable translational rejuvenation efforts in humans.

核心D-摘要