Detection of microsatellite instability biomarkers for therapeutic clinical trial eligibility

检测治疗性临床试验资格的微卫星不稳定性生物标志物

基本信息

  • 批准号:
    9313523
  • 负责人:
  • 金额:
    $ 35.42万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-09-25 至 2018-09-24
  • 项目状态:
    已结题

项目摘要

Project Title: Detection of microsatellite instability biomarkers for therapeutic clinical trial eligibility Project Summary When enrolling patients with advanced cancer in clinical trials, there is a need for clinical grade diagnostics to detect predictive biomarkers for novel immunotherapies. Microsatellite instability (MSI) has been identified as a novel predictive biomarker for cancer immunotherapy. Detecting MSI is currently accomplished with multiple redundant assays including immunohistochemistry for four proteins in the DNA repair pathway (MLH1, MSH2, MSH6, PMS2) and PCR for five selected microsatellite positions on finite tumor specimens (2). These diagnostic tests have been optimized for patients with colorectal cancer suspected of having germline Lynch Syndrome. Unfortunately, these assays oftentimes exhaust finite clinical specimens. The use of next generation sequencing (NGS)-based tests has expanded the profile of molecular diagnostics and raises the potential to integrate detection of MSI and eliminate the requirement for multiple parallel tests. While patients undergo genomic testing for other types of mutations such as point mutations, there is a critical need to augment current assays to include detection of microsatellite instability given its predictive value. Furthermore, current microsatellite detection algorithms have been specifically developed for a small number of cancer types and therefore are not accurate for MSI testing in most cancers. Our Clinical Laboratory Improvement Amendments (CLIA)--compliant Cancer Genomics Lab has extensive experience in developing clinical grade tumor sequencing, bioinformatics, and mutation-driven trials (3-6). We hypothesize that targeted DNA sequencing and analysis enables the detection of microsatellite instability in patient specimens from diverse cancer types. During the UH2 Phase of Analytic Validation, we will determine the sensitivity, specificity, reproducibility and reportable ranges of a targeted DNA microsatellite sequencing assay, MSI-Dx, utilizing clinical tumor specimens (Aim 1). We will demonstrate scalability, rapid turnaround, and use of MSI-Dx on a desktop sequencer. During the UH3 Phase of Clinical Validation, the MSI-Dx assay will be applied on a diverse collection of samples comprised of known MSI-H tumors including colorectal, endometrial, and other cancer types (Aim 2). Further, we will utilize the MSI-Dx assay for patients enrolled in a real time clinical tumor sequencing study (Aim 3). Importantly, MSI-Dx can be integrated with other NGS-based testing strategies. This assay will have a broad therapeutic impact by facilitating precision medicine clinical trials for patients with MSI-H tumors.
项目标题:检测治疗临床试验合格的微卫星不稳定生物标记物 项目摘要 当登记晚期癌症患者参加临床试验时,需要临床分级诊断以 检测新型免疫疗法的预测性生物标记物。微卫星不稳定性(MSI)被认为是一种 癌症免疫治疗的新型预测生物标记物。检测MSI目前是通过多个 冗余分析包括DNA修复途径中四种蛋白的免疫组织化学(MLH1,MSH2, MSH6、PMS2)和在有限肿瘤标本上选择的五个微卫星位置的PCR(2)。这些 对怀疑患有生殖系林奇的结直肠癌患者的诊断测试进行了优化 综合症。不幸的是,这些检测往往耗尽有限的临床标本。Next的用法 基于世代测序(NGS)的测试扩展了分子诊断的范围,并提高了 有可能整合MSI的检测并消除对多个并行测试的需求。当病人 接受其他类型的突变的基因组测试,如点突变,迫切需要 考虑到微卫星的预测价值,增加电流分析以包括微卫星不稳定性的检测。此外, 目前的微卫星检测算法是专门为少数癌症类型开发的 因此对大多数癌症的MSI检测并不准确。我们的临床实验室改进 修正(CLIA)--符合标准的癌症基因组实验室在开发临床分级方面拥有丰富的经验 肿瘤测序、生物信息学和突变驱动试验(3-6)。我们假设以DNA为靶标 测序和分析使检测患者样本中的微卫星不稳定性成为可能 癌症类型多样。在分析验证的UH2阶段,我们将确定灵敏度, 靶向DNA微卫星测序分析MSI-Dx的特异性、重复性和可报告范围, 利用临床肿瘤标本(目标1)。我们将演示MSI-Dx的可扩展性、快速周转和使用 在台式测序仪上。在UH3临床验证阶段,MSI-Dx检测将应用于 各种样本的收集,包括已知的MSI-H肿瘤,包括结直肠、子宫内膜和其他 癌症类型(目标2)。此外,我们将对登记在实时临床肿瘤中的患者使用MSI-Dx检测 测序研究(目标3)。重要的是,MSI-Dx可以与其他基于NGS的测试策略集成。这 通过促进精准医学临床试验,化验将产生广泛的治疗影响 MSI-H肿瘤。

项目成果

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Sameek Roychowdhury其他文献

Sameek Roychowdhury的其他文献

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{{ truncateString('Sameek Roychowdhury', 18)}}的其他基金

Liquid Biopsy for Rapid Detection and Real Time Monitoring of FGFR-altered Cancers
液体活检用于快速检测和实时监测 FGFR 改变的癌症
  • 批准号:
    10922903
  • 财政年份:
    2021
  • 资助金额:
    $ 35.42万
  • 项目类别:
Liquid Biopsy for Rapid Detection and Real Time Monitoring of FGFR-altered Cancers
液体活检用于快速检测和实时监测 FGFR 改变的癌症
  • 批准号:
    10282372
  • 财政年份:
    2021
  • 资助金额:
    $ 35.42万
  • 项目类别:
Detection of microsatellite instability biomarkers for therapeutic clinical trial eligibility
检测治疗性临床试验资格的微卫星不稳定性生物标志物
  • 批准号:
    9912125
  • 财政年份:
    2017
  • 资助金额:
    $ 35.42万
  • 项目类别:
Assay Validation of Targeted RNA sequencing to Detect Kinase Gene Fusions
用于检测激酶基因融合的靶向 RNA 测序的分析验证
  • 批准号:
    9762047
  • 财政年份:
    2016
  • 资助金额:
    $ 35.42万
  • 项目类别:
Assay Validation of Targeted RNA sequencing to Detect Kinase Gene Fusions
用于检测激酶基因融合的靶向 RNA 测序的分析验证
  • 批准号:
    9041381
  • 财政年份:
    2016
  • 资助金额:
    $ 35.42万
  • 项目类别:
Assay Validation of Targeted RNA sequencing to Detect Kinase Gene Fusions
用于检测激酶基因融合的靶向 RNA 测序的分析验证
  • 批准号:
    10005278
  • 财政年份:
    2016
  • 资助金额:
    $ 35.42万
  • 项目类别:
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