Functional Dynamics of Cytochrome P4503A4
细胞色素 P4503A4 的功能动力学
基本信息
- 批准号:9638812
- 负责人:
- 金额:$ 49.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-09-18 至 2022-06-30
- 项目状态:已结题
- 来源:
- 关键词:Active SitesAdverse effectsAffinityAnaerobic BacteriaBehaviorBindingBinding SitesBiochemicalBiochemistryBiophysicsBromocriptineCYP3A4 geneCatalysisCessation of lifeCharacteristicsClinical ResearchCompetenceComplexCrystallizationCytochromesDataDeuteriumDrug DesignDrug InteractionsDrug TargetingEquilibriumExhibitsFaceFluconazoleFrequenciesHemeHepaticHumanHydrogenHydrogen BondingHydrogen PeroxideIn VitroKineticsKnowledgeLigand BindingLigandsLinkLipid BilayersLipidsMapsMass Spectrum AnalysisMeasuresMechanicsMembraneMembrane LipidsMethodsMidazolamModelingMolecularMolecular ConformationMonitorMotionOpticsOxidation-ReductionPathway interactionsPharmaceutical PreparationsPharmacologyPlayProcessProlinePropertyProtein DynamicsProtein IsoformsReactionRecording of previous eventsRegulationResolutionRoleSourceSpecificityStructureToxic effectUncertaintyWaterWorkXenobioticsbasecofactordetoxicationdrug clearancedrug metabolismexperimental studyimprovedinhibitor/antagonistinterestmolecular dynamicsmolecular mechanicsnanodisknovelpredictive modelingpreventquantumresponsetraittranslational model
项目摘要
Project Summary
The proposed work aims to clarify longstanding mechanistic uncertainties about drug metabolizing cytochrome
P450s (CYPs). These CYPs are extraordinarily substrate promiscuous and they are major determinants of
xenobiotic detoxication, drug metabolism and drug interactions. Because of their role in drug metabolism, they
are also drug targets, wherein their pharmacological manipulation can afford control of therapy with other drugs.
One focus here is the knowledge gap concerning the relationship between ligand-dependent heme spin state
and catalytic properties. In the CYP canonical catalytic reaction cycle developed for substrate specific
isoforms, the substrate displaces heme bound water and causes a shift from low spin to high spin heme with
concomitant shift in the heme properties that facilitate reduction and progress through the catalytic cycle. In
contrast, with drug metabolizing CYPs, many drugs cause no shift to high spin heme, but they are efficiently
metabolized or cause hydrogen peroxide formation, both of which require progression through the catalytic
cycle. Recent results demonstrate that many of these substrates hydrogen bond to the axial water, rather than
displace it. The catalytic properties of these water-bridged complexes have not been determined. Therefore,
the proposed work will determine for CYP3A4 the heme redox properties and the catalytic competence of
water-bridged complexes, using computational approaches and advanced biochemical methods including
spectropotentiometry and stopped-flow reaction kinetics. Computational approaches will also be employed to
understand the effect of water-bridged complexes on heme reduction processes.
A second focus of the proposed work aims to clarify the role of conformational dynamics in the complex
allosteric behavior of CYPs and their remarkable substrate promiscuity. Both traits are linked to the protein
dynamics, which remain poorly characterized, and unclarified by the available crystal structures. The allosteric
properties confound prediction of drug clearance and drug interactions, so there is great interest in translational
models that better predict drug interactions based on refined allosteric models. Here, the experimental
methods of hydrogen-deuterium exchange mass spectrometry (H/DX) and pre-steady state ligand binding
methods with CYP3A4 in lipid bilayer nanodiscs are combined with computational approaches such as
accelerated Molecular Dynamics simulations (aMD) and steered molecular dynamics. The proposed studies fill
a significant gap in understanding the, previously experimentally inaccessible, CYP dynamics in a lipid
membrane and the role of conformational dynamics in achieving substrate promiscuity and allostery.
项目摘要
这项拟议的工作旨在澄清药物代谢细胞色素的长期机制不确定性。
P450(细胞色素P450)。这些环磷酰胺是非常混杂的底物,它们是
异源生物解毒、药物代谢和药物相互作用。由于它们在药物新陈代谢中的作用,它们
也是药物靶点,其中它们的药理操作可以提供对其他药物治疗的控制。
这里的一个焦点是关于配体依赖的血红素自旋态之间的关系的知识差距
和催化性能。在为底物特定开发的CYP正则催化反应循环中
异构体,底物取代血红素结合水,并导致从低自旋到高自旋血红素的转变
伴随而来的血红素性质的变化,促进了催化循环的还原和进展。在……里面
相比之下,对于药物代谢的Cyps,许多药物不会导致向高自旋血红素的转变,但它们是有效的
代谢或引起过氧化氢的形成,两者都需要通过催化剂进行
周而复始。最近的结果表明,这些底物中的许多氢键与轴向水结合,而不是
把它移开。这些水桥络合物的催化性能尚未确定。因此,
拟议的工作将确定CYP3A4的血红素氧化还原性质和催化活性
使用计算方法和先进的生化方法,包括
光谱电位法和停流反应动力学。还将使用计算方法来
了解水桥络合物对血红素还原过程的影响。
拟议工作的第二个重点是澄清构象动力学在复合体中的作用
胞外多糖的变构行为及其显著的底物混杂。这两个特征都与蛋白质有关
动力学,仍然没有得到很好的描述,并且没有被现有的晶体结构所阐明。变构
性质混淆了药物清除和药物相互作用的预测,因此对翻译的兴趣很大。
基于改进的变构模型更好地预测药物相互作用的模型。这里,试验性的
氢-氚交换质谱学(H/DX)和稳态前配基结合方法
在脂质双层纳米盘中使用CYP3A4的方法与计算方法相结合,例如
加速分子动力学模拟(AMD)和转向分子动力学。建议的研究填补了
在理解脂质中以前无法通过实验获得的CYP动力学方面的重大差距
膜和构象动力学在实现底物混杂和变构中的作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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WILLIAM M ATKINS其他文献
WILLIAM M ATKINS的其他文献
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{{ truncateString('WILLIAM M ATKINS', 18)}}的其他基金
Drug, Nucleotide, and Lipid Interactions with P-glycoprotein
药物、核苷酸和脂质与 P-糖蛋白的相互作用
- 批准号:
10672242 - 财政年份:2022
- 资助金额:
$ 49.63万 - 项目类别:
Functional Dynamics of Cytochrome P4503A4
细胞色素 P4503A4 的功能动力学
- 批准号:
10205098 - 财政年份:2018
- 资助金额:
$ 49.63万 - 项目类别:
P450-Base Drug Interactions with Low Spin Drugs
P450 基药物与低自旋药物的相互作用
- 批准号:
8716902 - 财政年份:2013
- 资助金额:
$ 49.63万 - 项目类别:
P450-Base Drug Interactions with Low Spin Drugs
P450 基药物与低自旋药物的相互作用
- 批准号:
9120388 - 财政年份:2013
- 资助金额:
$ 49.63万 - 项目类别:
P450-Base Drug Interactions with Low Spin Drugs
P450 基药物与低自旋药物的相互作用
- 批准号:
8740514 - 财政年份:2013
- 资助金额:
$ 49.63万 - 项目类别:
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