CORE B: METABOLIC AND MOLECULAR PHYSIOLOGY CORE

美国国立卫生研究院发起了集中专业知识、仪器和设施的努力，以促进对基因工程小鼠、组织和细胞的全面评估，这提高了我们对功能基因组学和疾病病理生物学的理解。代谢和分子生理学核心（MMPC）为加州大学洛杉矶分校、加州大学圣地亚哥分校、雪松西奈医学院和索尔克研究所的研究人员提供了一系列最先进、最具成本效益的分子和生理检测，这些检测在国家表型中心是不容易获得的。MMPC分为四个子核心：A)胰岛素敏感性和代谢，B)氧化代谢和线粒体生物学，C)脂质地图-脂质组学和D)炎症信号，每个子核心提供从实验设计到数据解释和整合的各种主题的广泛培训和咨询。具体来说，MMPC提供的服务包括：运动、摄食行为、间接量热、身体组成、葡萄糖/胰岛素耐量、胰岛素作用、底物代谢和氧化能力、线粒体功能、循环和组织脂质（脂质组学）、循环激素/脂肪因子/细胞因子和糖尿病并发症。MMPC维护着一个组织生物库，以及大量表型技术标准方案的综合数据库，以及各种基因工程小鼠的表型结果。MMPC的领导包括来自核受体生物学、脂质代谢、炎症、脂肪细胞和骨骼肌生物学领域的顶尖研究人员，包括：Peter Tontonoz、Andrea Hevener、Karen Reue、Rajendra Tangirala、Edward Dennis、Oswald Quehenberger。MMPC的优势包括其领导层的全面和互补的专业知识，以及生产力和高影响力科学出版物的卓越记录。MMPC团队的合作精神营造了一个合作的环境，并支持与其他DRC核心和机构资源良好协调的服务。MMPC的中心目标是推进DRC成员在前沿代谢分析方面的科学能力，通过加强从细胞，组织和小鼠到人的研究思想的翻译，提高整体研究质量。

NIH-initiated efforts to centralize expertise, instrumentation, and facilities to promote comprehensive evaluation of genetically engineered mice, tissues and cells has improved our understanding of functional genomics and disease pathobiology. The Metabolic and Molecular Physiology Core (MMPC) provides investigators at UCLA, UCSD, Cedars-Sinai, and the Salk Institute with a series of state-of-the-art and cost-effective molecular and physiological assays not readily available from national phenotyping centers. The MMPC is divided into four sub-cores: A) Insulin sensitivity and metabolism, B) Oxidative metabolism and mitochondrial biology, C) LIPID MAPS-lipidomics, and D) Inflammatory siganling, and each sub-core offers extensive training and consultation on a variety of topics from experimental design to data interpretation and integration. Specifically, the MMPC provides services to assess: movement, feeding behavior, indirect calorimetry, body composition, glucose/insulin tolerance, insulin action, substrate metabolism and oxidative capacity, mitochondrial function, circulating and tissue lipids (lipidomics), circulating hormones / adipokines / cytokines, and diabetes complications. The MMPC maintains a tissue bio-bank as well as a comprehensive database of standard protocols for a vast number of phenotyping techniques, and phenotypic outcomes for a wide variety of genetically engineered mice. The MMPC leadership includes top investigators from the fields of nuclear receptor biology, lipid metabolism, inflammation, and adipocyte and skeletal muscle biology including: Peter Tontonoz, Andrea Hevener, Karen Reue, Rajendra Tangirala, Edward Dennis, Oswald Quehenberger. Strengths of the MMPC include the well-rounded and complementary expertise of its leadership and the exceptional track record of productivity and high impact scientific publications. The collaborative spirit ofthe MMPC team fosters a collegial environment and supports service well-coordinated with other DRC cores and institutional resources. The central goal ofthe MMPC is to advance the scientific capabilities ofthe DRC membership in leading-edge metabolic analysis to improve overall research quality with enhanced translation of research ideas from cell, tissue and mouse to man.