CORE B: METABOLIC AND MOLECULAR PHYSIOLOGY CORE

核心 B：代谢和分子生理学核心

• 批准号: 9283520
• 负责人: Andrea L Hevener
• 金额: $ 32.66万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2018-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9283520
• 关键词: Adipocytes; Animals; Area; Arterial Fatty Streak; Arts; Biological Assay; Biology; Body Composition; Cell Respiration; Cells; Closure by clamp; Collaborations; Communities; Complications of Diabetes Mellitus; Consult; Consultations; Country; Data; Data Analyses; Databases; Diabetes Mellitus; Diet; Disease; Dual-Energy X-Ray Absorptiometry; Environment; Epigenetic Process; Evaluation; Experimental Designs; Faculty; Feeding behaviors; Fostering; Gas Chromatography; Gene Targeting; Genetic; Genetically Engineered Mouse; Genomics; Glucose; Glycerol; Glycerophospholipids; Goals; Hormones; Human; Human Genetics; Indirect Calorimetry; Inflammation; Inflammatory; Institutes; Institution; Insulin; Intervention; Knockout Mice; Laboratories; Leadership; Lipids; Liquid Chromatography; Los Angeles; Magnetic Resonance Imaging; Mass Spectrum Analysis; Metabolic; Metabolism; Methodology; Microscopy; Mission; Mitochondria; Molecular; Molecular Analysis; Movement; Mus; Nuclear Receptors; Obesity; Outcome; Oxygen Consumption; Pathogenesis; Patient Care; Peptides; Pharmacologic Substance; Phenotype; Physiological; Physiology; Plasma; Preparation; Procedures; Process; Productivity; Protocols documentation; Publications; Quality Control; Recruitment Activity; Research; Research Personnel; Resources; Rodent; Sampling; Scientific Advances and Accomplishments; Series; Services; Signal Transduction; Skeletal Muscle; Sphingolipids; Standardization; Sterols; Strategic Planning; Techniques; Technology; Time; Tissues; Training; Transgenic Mice; Translational Research; Translations; Travel; United States National Institutes of Health; Work; adipokines; animal tissue; assay development; base; bench to bedside; biobank; chemokine; cost; cost effective; cytokine; data integration; experience; extracellular; fatty acid metabolism; flexibility; functional genomics; improved; in vivo; insight; instrumentation; insulin sensitivity; insulin tolerance; lipid metabolism; macrophage; man; metabolic abnormality assessment; metabolic phenotype; monocyte; novel; outreach; prenol; prevent; synergism; therapeutic target

NIH-initiated efforts to centralize expertise, instrumentation, and facilities to promote comprehensive evaluation of genetically engineered mice, tissues and cells has improved our understanding of functional genomics and disease pathobiology. The Metabolic and Molecular Physiology Core (MMPC) provides investigators at UCLA, UCSD, Cedars-Sinai, and the Salk Institute with a series of state-of-the-art and cost-effective molecular and physiological assays not readily available from national phenotyping centers. The MMPC is divided into four sub-cores: A) Insulin sensitivity and metabolism, B) Oxidative metabolism and mitochondrial biology, C) LIPID MAPS-lipidomics, and D) Inflammatory siganling, and each sub-core offers extensive training and consultation on a variety of topics from experimental design to data interpretation and integration. Specifically, the MMPC provides services to assess: movement, feeding behavior, indirect calorimetry, body composition, glucose/insulin tolerance, insulin action, substrate metabolism and oxidative capacity, mitochondrial function, circulating and tissue lipids (lipidomics), circulating hormones / adipokines / cytokines, and diabetes complications. The MMPC maintains a tissue bio-bank as well as a comprehensive database of standard protocols for a vast number of phenotyping techniques, and phenotypic outcomes for a wide variety of genetically engineered mice. The MMPC leadership includes top investigators from the fields of nuclear receptor biology, lipid metabolism, inflammation, and adipocyte and skeletal muscle biology including: Peter Tontonoz, Andrea Hevener, Karen Reue, Rajendra Tangirala, Edward Dennis, Oswald Quehenberger. Strengths of the MMPC include the well-rounded and complementary expertise of its leadership and the exceptional track record of productivity and high impact scientific publications. The collaborative spirit ofthe MMPC team fosters a collegial environment and supports service well-coordinated with other DRC cores and institutional resources. The central goal ofthe MMPC is to advance the scientific capabilities ofthe DRC membership in leading-edge metabolic analysis to improve overall research quality with enhanced translation of research ideas from cell, tissue and mouse to man.

NIH发起的集中专业知识，仪器和设施，以促进基因工程小鼠，组织和细胞的综合评价的努力，提高了我们对功能基因组学和疾病病理生物学的理解。代谢和分子生理学核心（MMPC）为UCLA，UCSD，Cedars-Sinai和Salk研究所的研究人员提供了一系列国家表型中心不容易获得的最先进和具有成本效益的分子和生理测定。MMPC分为四个子核心：A）胰岛素敏感性和代谢，B）氧化代谢和线粒体生物学，C）LIPID MAPS-脂质组学和D）炎症信号，每个子核心都提供从实验设计到数据解释和整合的各种主题的广泛培训和咨询。具体而言，MMPC提供评估服务：运动、进食行为、间接热量测定、身体组成、葡萄糖/胰岛素耐受性、胰岛素作用、底物代谢和氧化能力、线粒体功能、循环和组织脂质（脂质组学）、循环激素/脂肪因子/细胞因子和糖尿病并发症。MMPC维护一个组织生物库以及一个综合数据库，其中包含大量表型分析技术的标准方案和各种基因工程小鼠的表型结果。MMPC领导层包括来自核受体生物学、脂质代谢、炎症、脂肪细胞和骨骼肌生物学领域的顶级研究人员，包括：Peter Tontonoz、Andrea Hevener、Karen Reue、Rajendra Tangirala、Edward Dennis、Oswald Quehenberger。MMPC的优势包括其领导层的全面和互补的专业知识，以及生产力和高影响力科学出版物的卓越记录。MMPC团队的协作精神促进了学院环境，并支持与其他DRC核心和机构资源良好协调的服务。MMPC的中心目标是提高DRC成员在前沿代谢分析方面的科学能力，以提高整体研究质量，增强从细胞，组织和小鼠到人类的研究思想的翻译。