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Genetics of Vector Populations: Aedes aegypi

病媒种群遗传学：埃及伊蚊

基本信息

批准号：
9064743
负责人：
Jeffrey R POWELL
金额：
$ 41.63万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-06-15 至 2018-05-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We have identified more than 40,000 single nucleotide polymorphisms (SNPs) in 16 samples of Aedes aegypti from around the world, as well as a sample of its closest relative, Ae. mascarensis. This was done using a technique that is efficient in identifying SNPs (RAD-tags), but this methodology is not applicable for routine genotyping. We propose to develop a SNP chip based on these identified SNPs that can be applied routinely for reliable genotyping in a time- and cost-effective manner. Our own interests are on the population genetics and ancestry level and we will focus on a chip designed specifically for our studies. A by-product of the work will be more extensive data upon which other groups could design a chip for genome wide association studies; all data will be publically available. The first part of the project is development of the population chip. This will proceed i three steps: (1) From the >40,000 available SNPs, identify ~5,000 that have the most variation across the species' distribution and reliably genotype on SNP chips. (2) From these 5,000, use theoretical analyses to identify ~750 that capture the population/ancestry information of the complete data set. (2) Perform crosses to confirm the Mendelian (single-copy, nuclear) nature of these 750, with the expectation that at least 500 will be Mendelian and form the basis of the population SNP chip. The second part of the project is application of this chip. Four projects are proposed: (1) Perform a global population genetics study to determine the genetic diversity, patterns, and relatedness of populations from around the world; collections are already in hand for this. (2) Study five ecologically and geographically diverse populations through time to determine the genetic stability of Ae. aegypti populations as well as effective population sizes. (3) Genotype common lab strains to determine where they fit into the genetic diversity of the species and how homogeneous or heterogeneous they are among isolates from different laboratories. (4) Genotype populations in West Africa that have recently colonized urban centers and compare them to nearby sylvan populations that are the likely source of the "domestication" event; independent replicate cases will allow us to identify parallel genetic changes that would give insight into the genetic nature of the domestication process.

描述（由申请人提供）：我们已经在来自世界各地的16个埃及伊蚊样本中鉴定出超过40,000个单核苷酸多态性（snp），以及它最近的亲戚伊蚊的样本。mascarensis。这是使用一种有效识别snp （rad标签）的技术完成的，但这种方法不适用于常规基因分型。我们建议开发一种基于这些已鉴定的SNP的SNP芯片，该芯片可以在时间和成本效益的方式下常规应用于可靠的基因分型。我们自己的兴趣是在群体遗传学和祖先水平，我们将重点放在一个芯片专门为我们的研究。这项工作的副产品将是更广泛的数据，其他团队可以根据这些数据设计基因组广泛关联研究的芯片；所有的数据都是公开的。项目的第一部分是人口芯片的开发。这将分三步进行：(1)从40000个可用的SNP中，找出5000个在整个物种分布中变异最大的SNP，并在SNP芯片上可靠地进行基因型分析。(2)从这5000个样本中，通过理论分析找出750个样本能够捕捉到完整数据集的种群/祖先信息。(2)进行杂交以确认这750个基因的孟德尔基因（单拷贝，核）性质，期望至少有500个基因是孟德尔基因，并形成群体SNP芯片的基础。项目的第二部分是该芯片的应用。(1)开展全球种群遗传学研究，确定世界各地种群的遗传多样性、遗传模式和亲缘关系；这方面的收藏品已经准备好了。(2)研究5个不同生态地理种群的遗传稳定性。埃及伊蚊种群以及有效种群规模。(3)对常见实验室菌株进行基因分型，以确定它们在物种遗传多样性中的位置，以及它们在不同实验室分离物中的同质或异质程度。(4)最近在西非殖民城市中心的基因型种群，并将其与附近可能是“驯化”事件来源的森林种群进行比较；独立的重复案例将使我们能够识别平行的遗传变化，从而深入了解驯化过程的遗传本质。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genetic structure of the mosquito Aedes aegypti in local forest and domestic habitats in Gabon and Kenya.

加蓬和肯尼亚当地森林和家庭栖息地埃及伊蚊的遗传结构。

DOI：
10.1186/s13071-020-04278-w
发表时间：
2020
期刊：
Parasites & vectors
影响因子：
3.2
作者：
Xia,Siyang;Cosme,LucianoV;Lutomiah,Joel;Sang,Rosemary;Ngangue,MarcF;Rahola,Nil;Ayala,Diego;Powell,JeffreyR
通讯作者：
Powell,JeffreyR

Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses.