Nanopore based profiling of epigenetic state
基于纳米孔的表观遗传状态分析
基本信息
- 批准号:10214994
- 负责人:
- 金额:$ 63.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAntibodiesAreaAzidesBar CodesBindingBinding ProteinsBinding SitesBiologyCellsChIP-seqChemicalsChromatinColorComplexCountryCytosineDNADNA Modification ProcessDNA SequenceDNA Sequence AlterationDNA sequencingDNA-Binding ProteinsDNA-Protein InteractionDataDetectionDevelopmentEnhancersEnzymesEpigenetic ProcessFundingGene ExpressionGene Expression RegulationGenerationsGenomeGrantHeterogeneityHuman GenomeIceIndividualLabelLengthLogisticsMalignant NeoplasmsMeasurementMeasuresMethodologyMethodsMethylationMethyltransferaseModificationMolecularMutationNuclearOrganic ChemistryOrganismPeptide Sequence DeterminationPhasePopulationProteinsRNARepetitive SequenceResolutionSignal TransductionSiteSomatic CellStimulusTechnologyTimeTissuesTrainingTranslatingVariantWorkanalysis pipelinebasecofactorcombinatorialepigenetic profilingepigenetic variationepigenomeepigenomicsglycosylationhistone modificationhuman diseasehuman reference genomeinsightnanoporenovelpromoterresponsesequencing platformsingle moleculestemtool
项目摘要
Project Summary:
Though a reference human genome and even excellent characterization of the epigenome (ENCODE, Epigenetics
Roadmap, 4D Nucleome Project) has been generated, we still lack a clear understanding of how the different
facets of the epigenome collaborate to control gene expression. With the advent of affordable DNA-sequencing
technologies, methods have been developed for examining nuclear organization, protein-DNA interaction,
chromatin accessibility, and methylation state. But these methods generally interrogate only one aspect of the
epigenome at a time and reads are typically too short to provide critical correlative information. We propose the
development of a novel epigenetic characterization methodology, in this round of funding focusing on protein-
DNA interaction through leveraging the long reads and modified bases detectable with a nanopore sequencing
platform. We will enhance the signal from subtle modifications being used to profile protein-DNA interactions.
We are developing a method to insert sequence tags (nanoTUBI) near the sites of protein-DNA binding. And we
will implement these methods focusing on long read sequencing, gaining insights into long correlative
measurements and heretofore unexplored repetitive areas, phasing the entire epigenome. These tools and
analysis pipelines will grant new insights into mechanisms of gene regulation, as well as their implications for
human disease.
项目总结:
通过参考人类基因组,甚至很好地描述了表观基因组(ENCODE,表观遗传学
路线图,4D核基因组计划)已经产生,我们仍然缺乏明确的认识
表观基因组的各个方面协同控制基因的表达。随着负担得起的DNA测序技术的出现
已经开发出检测核组织、蛋白质-DNA相互作用、
染色质可及性和甲基化状态。但这些方法通常只询问
表观基因组和阅读通常太短,不能提供关键的相关信息。我们建议
开发一种新的表观遗传学表征方法,在这一轮以蛋白质为重点的资助中
通过利用纳米孔测序可检测到的长阅读和修饰碱基来实现DNA相互作用
站台。我们将增强用于描述蛋白质-DNA相互作用的微妙修饰的信号。
我们正在开发一种在蛋白质-DNA结合位点附近插入序列标签(NanTUBI)的方法。而我们
我将实施这些方法,重点放在长阅读排序上,获得对长相关的见解
测量和迄今未探索的重复区域,分阶段完成整个表观基因组。这些工具和
分析管道将给予对基因调控机制的新见解,以及它们对
人类疾病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Winston George Timp其他文献
Winston George Timp的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Winston George Timp', 18)}}的其他基金
Direct nanopore detection of modified RNA to probe structure and dynamics
直接纳米孔检测修饰的 RNA 以探测结构和动力学
- 批准号:
9919610 - 财政年份:2019
- 资助金额:
$ 63.4万 - 项目类别:
Molecular Dissection of Colorectal CanceR: Effects of Loss of Imprinting of IGF2
结直肠癌的分子解剖 R:IGF2 印记丢失的影响
- 批准号:
8201096 - 财政年份:2010
- 资助金额:
$ 63.4万 - 项目类别:
Molecular Dissection of Colorectal CanceR: Effects of Loss of Imprinting of IGF2
结直肠癌的分子解剖 R:IGF2 印记丢失的影响
- 批准号:
7806721 - 财政年份:2010
- 资助金额:
$ 63.4万 - 项目类别:
相似海外基金
University of Aberdeen and Vertebrate Antibodies Limited KTP 23_24 R1
阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
- 批准号:
10073243 - 财政年份:2024
- 资助金额:
$ 63.4万 - 项目类别:
Knowledge Transfer Partnership
Role of Natural Antibodies and B1 cells in Fibroproliferative Lung Disease
天然抗体和 B1 细胞在纤维增生性肺病中的作用
- 批准号:
10752129 - 财政年份:2024
- 资助金额:
$ 63.4万 - 项目类别:
CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
- 批准号:
2339201 - 财政年份:2024
- 资助金额:
$ 63.4万 - 项目类别:
Continuing Grant
Isolation and characterisation of monoclonal antibodies for the treatment or prevention of antibiotic resistant Acinetobacter baumannii infections
用于治疗或预防抗生素耐药鲍曼不动杆菌感染的单克隆抗体的分离和表征
- 批准号:
MR/Y008693/1 - 财政年份:2024
- 资助金额:
$ 63.4万 - 项目类别:
Research Grant
Discovery of novel nodal antibodies in the central nervous system demyelinating diseases and elucidation of the mechanisms through an optic nerve demyelination model
发现中枢神经系统脱髓鞘疾病中的新型节点抗体并通过视神经脱髓鞘模型阐明其机制
- 批准号:
23K14783 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Elucidation of the mechanisms controlling the physicochemical properties and functions of supercharged antibodies and development of their applications
阐明控制超电荷抗体的理化性质和功能的机制及其应用开发
- 批准号:
23KJ0394 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Developing first-in-class aggregation-specific antibodies for a severe genetic neurological disease
开发针对严重遗传神经系统疾病的一流聚集特异性抗体
- 批准号:
10076445 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别:
Grant for R&D
PLA2G2D Antibodies for Cancer Immunotherapy
用于癌症免疫治疗的 PLA2G2D 抗体
- 批准号:
10699504 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别:
Genetic adjuvants to elicit neutralizing antibodies against HIV
基因佐剂可引发抗艾滋病毒中和抗体
- 批准号:
10491642 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别:
Novel Immunogens to Elicit Broadly Cross-reactive Antibodies That Target the Hemagglutinin Head Trimer Interface
新型免疫原可引发针对血凝素头三聚体界面的广泛交叉反应抗体
- 批准号:
10782567 - 财政年份:2023
- 资助金额:
$ 63.4万 - 项目类别: