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Divergent Functional and Metabolic Development of the Infant Microbiome

婴儿微生物组的不同功能和代谢发育

基本信息

批准号：
10214525
负责人：
Susan Veronica Lynch
金额：
$ 39.52万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-10 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10214525
关键词：
2 year old Allergic Arizona Asthma Bacteria Biological Assay Birth Border Community Cells Childhood Childhood Asthma Data Development Electrical Resistance Environment Environmental Exposure Epithelial Exhibits FOXP3 gene Feces Frequencies Genes Genetic House Dust Human IL4 gene IgE Immune Immunity In Vitro Infant Infant Development Inherited Intestines Life Location Meconium Medicine Metabolic Metagenomics Mexican Mexico Microbe Mothers Nature Neonatal Newborn Infant Outcome Pattern Population Preparation Prevalence Production Residencies Resolution Ribosomal RNA Risk Sampling Shotguns Source T-Lymphocyte Testing Vagina Water Supply Wheezing Woman atopy base cohort drinking water experience fetal gut microbiome gut microbiota high risk high risk infant immune function immunological status immunoregulation in utero infancy infant gut microbiome inflammatory disease of the intestine maternal microbiome member metabolomics microbial microbial composition microbiome microbiome alteration microbiome components microbiome composition microbiome research microbiota mouse model neonate offspring peer pregnant prenatal stool sample transcriptome sequencing vaginal microbiota water sampling

项目摘要

BEAMS ABSTRACT: Project 2 Diminished early life environmental microbial exposures and perturbations to the infant gut microbiome are related to asthma outcomes in childhood. Mexican populations resident in Nogales, Mexico exhibit a four-fold decrease in childhood asthma rates compared with a population of comparable ancestry resident seventy miles away across the border in Tucson, Arizona. Our preliminary data has demonstrated that these cross-border populations experience significantly distinct microbial exposures in their environment (house dust and drinking water) and that the stool microbiota of 1-month old infants raised in these environments are differentially enriched for immunomodulatory bacterial genera. This suggests that microbial differentials in environmental and personal microbiomes relate to the cross-border disparity in asthma rates observed in this population. BEAMS proposes to comprehensively examine a population of Mexican mother-infant dyads resident in Nogales, Mexico or Tucson, Arizona and their environment in early life to determine how differences in environmental, maternal and infant microbial composition and function relate to asthma disparities in this cross-border population. Project 2 will examine at high-resolution the functional attributes of the microbiome of a subset of paired maternal prenatal (vaginal and stool) and infant gut (longitudinally collected stool) samples collected from 200 mother-infant dyads, as well as their paired house dust and water samples using shotgun metagenomics, RNA-Seq and metabolomics. The study will examine functional differentials in inherited microbes and gut microbiome development in cross-border communities that relate to T2 wheezing at age 2 years (a robust predictor of asthma in later childhood) and immune development. These efforts aim to identify environmental, maternal and infant microbiome attributes that promote protection against asthma within Mexican populations.

梁摘要：项目2 减少生命早期环境微生物暴露和对婴儿肠道微生物组的干扰，与儿童哮喘的结果有关。居住在墨西哥诺加莱斯的墨西哥人口表现出四重与70英里外的可比祖先居民相比，儿童哮喘发病率降低在亚利桑那州的图森市我们的初步数据表明，这些跨境人群在其环境中经历显著不同的微生物暴露（房屋灰尘和饮用水）水），并且在这些环境中饲养的1个月大婴儿的粪便微生物群差异富集用于免疫调节细菌属。这表明环境和个人环境中的微生物差异微生物组与在这一人群中观察到的哮喘发病率的跨境差异有关。BEAMS建议全面检查居住在墨西哥诺加莱斯的墨西哥母婴二对人群，或图森，亚利桑那州和他们的环境在早期生活，以确定如何差异，环境，孕产妇和婴儿微生物组成和功能与这一跨境人群中哮喘差异有关。计划2 将以高分辨率检查配对母体产前微生物组的功能属性，（阴道和粪便）和婴儿肠道（纵向收集的粪便）样本，收集自200对母婴，以及他们配对的房屋灰尘和水样本，使用鸟枪宏基因组学，RNA测序和代谢组学该研究将检查遗传微生物和肠道微生物组的功能差异与2岁时T2喘息相关的跨境社区的发展（哮喘的一个可靠预测因素在童年后期）和免疫发育。这些努力旨在确定环境、产妇和婴儿微生物组属性，促进墨西哥人群中对哮喘的保护。