Core D Microbial Community Analysis Core

核心D将为项目1、2、3和4提供微生物群落组成概况，下一步- 世代生物标记物测序。拥有一个集中的处理核心进行测序将减少差异 由于gDNA提取、聚合酶链式反应扩增、测序步骤和数据过程中的样品处理 正常化。岩芯D非常有资格执行所产生的数据和 发表在我们之前的合作项目和初步数据中。这些研究使我们能够 证明母体微生物群向婴儿的垂直转移发生了，拓荒者社区 与低风险哮喘婴儿相比，高风险哮喘婴儿的微生物组发育轨迹明显不同 风险控制。核心D拥有人员专业知识、设备和能力，能够提供及时的高吞吐量 这项申请中的所有四个项目的微生物测序。

Core D will provide microbial community compositional profiling for Projects 1, 2, 3 and 4 with next- generation biomarker sequencing. Having a centralized processing core for sequencing will reduce variation due to sample processing during gDNA extraction, PCR amplification, sequencing steps and data normalization. Core D is highly qualified to perform such assays as is exemplified by the data generated and published in our previous collaborative projects and preliminary data. These studies have allowed us to demonstrate that vertical transfer of maternal microbiome to her infant occurs and that pioneer communities associate with distinct microbiome developmental trajectories in high-risk-for-asthma infants compared to low- risk controls. Core D has the personnel expertise, equipment and capacity to provide timely high-throughput microbial sequencing to all four projects in this application.