Core D Microbial Community Analysis Core

核心D将为项目1、2、3和4提供微生物群落组成分析， 生成生物标志物测序。有一个集中的处理核心进行排序将减少变异 由于gDNA提取、PCR扩增、测序步骤和数据过程中的样本处理 标准化核心D是高度合格的，以执行此类测定，如所生成的数据所示， 发表在我们以前的合作项目和初步数据。这些研究使我们能够 表明母体微生物组向婴儿的垂直转移发生， 与哮喘高危婴儿相比， 风险控制。核心D拥有人员专业知识、设备和能力，能够及时提供高通量 微生物测序的所有四个项目在这个应用程序。

Core D will provide microbial community compositional profiling for Projects 1, 2, 3 and 4 with next- generation biomarker sequencing. Having a centralized processing core for sequencing will reduce variation due to sample processing during gDNA extraction, PCR amplification, sequencing steps and data normalization. Core D is highly qualified to perform such assays as is exemplified by the data generated and published in our previous collaborative projects and preliminary data. These studies have allowed us to demonstrate that vertical transfer of maternal microbiome to her infant occurs and that pioneer communities associate with distinct microbiome developmental trajectories in high-risk-for-asthma infants compared to low- risk controls. Core D has the personnel expertise, equipment and capacity to provide timely high-throughput microbial sequencing to all four projects in this application.