Reagent/Service Core

试剂/服务核心

• 批准号: 9124862
• 负责人: CHUANSHU HUANG
• 金额: $ 30.39万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-12 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9124862
• 关键词: Animal Model; Animals; Antibodies; Biological Preservation; Biometry; Bladder; Bladder Tissue; Cancer cell line; Cause of Death; Cell Line; Collaborations; Common Core; Complementary DNA; Consult; Consultations; Data; Databases; Emergency Situation; Ensure; Environmental Medicine; Equipment and supply inventories; Event; Genetically Engineered Mouse; Genitourinary system; Human; In Vitro; Individual; Instruction; Laboratories; Link; Malignant Neoplasms; Malignant neoplasm of urinary bladder; Medical center; Molecular; Mus; Mutation; Nucleic Acids; Operating Rooms; Pathogenesis; Pathologic; Pathologist; Pathology; Pathway interactions; Plasmids; Process; Proteins; Quality Control; Reagent; Recombinant Proteins; Reporting; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Resources; Sampling; Services; Site; Specimen; Staging; TP53 gene; Tissue Sample; Tissues; Training; Training Support; Woman; base; biobank; cancer cell; carcinogenesis; cost effective; cost effectiveness; design; experience; human tissue; in vivo; knock-down; men; mouse model; mutant; overexpression; population health; professor; programs; repository; small hairpin RNA; statistics; success; time use; tool; tumorigenesis; vector

The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder carcinogenesis of both major pathways of low-grade, non-invasive and high-grade, invasive bladder cancer. In addition t o t h e intellectual and experimental synergies that link the individual projects into a cohesive program, each ofthe three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different stages of bladder cancer, different unique mouse models of bladder cancer developed by Dr. Xue-Ru Wu, recombinant proteins, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would It be an efficient use of time, money, and resources for each laboratory to Independently generate, procure, characterize, and maintain all these reagents. Instead, we will establish a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the Pis and guards against accidental loss of Invaluable reagents in emergency situations such as power outages (which we last experienced in 2003). Finally, the Core will also provide pathology and statistical support for the individual projects. In this section ofthe Program Project application, we describe these reagents, how the Core functions to control quality and maintain databases, the contribution ofthe core to the individual research projects, and a plan for prioritizing usage of core services. The specific aims ofthe Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects; ii) To collect human and mouse specimens and provide the necessary pathology support service; and iii) To provide dedicated statistical support.

包括该项目在内的三个研究项目都集中于了解导致膀胱癌发生的分子和细胞事件和机制，包括低级别、非侵袭性和高级别、侵袭性膀胱癌的主要途径。除了智力和实验的协同作用，将单个项目连接到一个有凝聚力的项目中，三个拟议的研究计划中的每一个都依赖于一套共同的关键试剂和服务，这些试剂和服务是他们成功的持续基础。这些包括但不限于一组代表膀胱癌不同阶段的培养细胞系，由Xue-Ru Wu博士开发的不同独特的膀胱癌小鼠模型，重组蛋白，核酸试剂，抗体和cDNA构建。显然，如果每个实验室都独立生产、采购、鉴定和维护所有这些试剂，这是没有意义的，也不是对时间、金钱和资源的有效利用。相反，我们将建立一个集中的试剂和服务核心，其目的之一是作为关键试剂的中央存储库，并为这些重要的研究工具提供中央供应，这些工具可以由本计划项目的所有参与者共享。除了成本效益之外，这种方法还提供了确保各个项目共享试剂质量的优势。从集中的核心提供细胞系，组织和动物模型的供应，消除了试剂处理的可变性，促进了三个单独项目之间的数据比较。此外，它促进了Pis之间的额外互动，并防止在停电等紧急情况下意外损失宝贵的试剂（我们上次经历停电是在2003年）。最后，核心还将为个别项目提供病理和统计支持。在项目申请的这一部分中，我们描述了这些试剂，核心功能如何控制质量和维护数据库，核心对各个研究项目的贡献，以及优先使用核心服务的计划。核心的具体目标是：i)维持集中供应和分发一套对支持单个组件项目的研究工作至关重要的通用核心试剂；ii)收集人类和小鼠标本，并提供必要的病理支持服务；及iii)提供专门的统计支援。