Demonstrate the molecular receptor and functions of dorsal organ warm cells in flies

展示果蝇背器官温细胞的分子受体和功能

• 批准号: 10308483
• 负责人: Lina Ni
• 金额: $ 33.6万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-01 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10308483
• 关键词: Afferent Neurons; Animals; Behavior; Behavioral; Behavioral Assay; Biological Models; Blood; Body Temperature; Calcium; Cells; Culicidae; Data; Disease Vectors; Dorsal; Drosophila genus; Electrophysiology (science); Ensure; Family; Feeding behaviors; Ganglia; Genetic; Genetic Models; Goals; High temperature of physical object; Image; Immunohistochemistry; Insecta; Label; Larva; Molecular; Names; Neurons; Organ; Physiological; Positioning Attribute; Property; Research; Role; System; Temperature; Temperature Sense; Testing; Vector-transmitted infectious disease; avoidance behavior; base; cold temperature; disorder control; driving force; experimental study; fly; human disease; insect disease vector; insect genetics; insight; member; novel; preference; receptor; receptor function; response; sensory mechanism; voltage; warm temperature

Summary – Lina Ni Animals depend on their temperature-sensing systems to avoid noxious thermal extremes and to seek optimal temperatures for survival. Temperature sensation is particularly relevant for small animals, such as insects, which rely on ambient temperatures to set their body temperatures. Many insect vectors of diseases, such as mosquitoes, respond to the temperature of their warm-blooded hosts and use it to guide the blood- feeding behaviors through which they transmit human diseases. Thus, it is important to identify the temperature- sensing molecules and neurons to help control disease vectors. Fruit flies are a suitable model system for these studies, because of their evolutionarily conserved temperature-sensing molecules with mosquito. In flies, many temperature-sensing systems possess a small number of temperature sensory neurons that master robust behaviors. Moreover, the powerful genetics developed in flies ensures the precise manipulation of temperature- sensing molecules and neurons. Preliminary studies from the PI’s lab discovered a set of previously unidentified warm-activated neurons in fly larvae, whose temperature-sensing molecules and functions are unknown. Preliminary data suggested that these neurons depended on a new class of warm-sensing molecules to detect warm temperatures. Besides a classic function of temperature-responsive neurons that determine temperature preference, these neurons may have an additional function to modulate their neighboring cool-activated neurons. Such modulation may result in change of physiological properties in neighboring neurons. The goal of this application is to identify the warm-sensing molecules in these warm-activated neurons, to demonstrate their functions in temperature preference, and to determine their modulatory effects on neighboring neurons. In addition to having preliminary data, we are particularly well-prepared to undertake the proposed research because of our extensive, and successful, track record of studying temperature-responsive molecules and sensory neurons in flies. Since the potential candidates of the warm-sensing molecules are conserved between flies and mosquitoes, this study might provide novel targets to control blood-feeding behaviors of mosquitoes and other disease vectors.

简介- Lina Ni 动物依靠它们的温度感应系统来避免有害的极端温度， 最适宜生存的温度温度感觉对于小动物特别相关，例如 昆虫，它们依靠环境温度来设定体温。许多疾病的昆虫媒介， 例如蚊子，对它们的温血宿主的温度做出反应，并利用它来引导血液- 它们通过进食行为传播人类疾病。因此，确定温度是很重要的- 传感分子和神经元来帮助控制疾病媒介。果蝇是一个合适的模型系统， 研究，因为他们的进化保守的温度传感分子与蚊子。在苍蝇中，许多 温度传感系统拥有少量的温度传感神经元， 行为。此外，果蝇强大的遗传学确保了对温度的精确控制- 传感分子和神经元。私家侦探实验室的初步研究发现了一组先前未确认的 果蝇幼虫中的温激活神经元，其温度传感分子和功能尚不清楚。 初步数据表明，这些神经元依赖于一类新的温敏分子来检测 温暖的温度。除了决定温度的温度反应神经元的经典功能之外 优选地，这些神经元可以具有调节其相邻的冷激活神经元的附加功能。 这种调制可以导致邻近神经元中生理特性的改变。这个目标 应用程序是确定这些温暖激活神经元中的温暖感受分子，以证明其 功能的温度偏好，并确定其对邻近神经元的调节作用。在 除了有初步的数据外，我们特别准备进行拟议的研究 由于我们在研究温度响应分子方面的广泛和成功的记录， 果蝇的感觉神经元。由于温敏分子的潜在候选者在 本研究为控制蚊虫吸血行为提供了新的靶点 和其他疾病媒介。