Integrative Analysis of Wilson's Disease

威尔逊病的综合分析

• 批准号: 9699645
• 负责人: SVETLANA LUTSENKO
• 金额: $ 12万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2021-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9699645
• 关键词: Cell Compartmentation; Cell Density; Cells; Environment; Evaluation; Grant; Hepatolenticular Degeneration; Kinetics; Manufacturer Name; Measurement; Microscope; Morphology; Norepinephrine; Oxidation-Reduction; Price; Regulation; Research; Signaling Molecule; Speed; Tissues; Wilson disease protein; discount; improved; instrument; live cell imaging; trafficking

PROJECT SUMMARY The LSM800 instrument will be heavily used for all specific aims of the parental grant. The studies that will be performed with the microscope include analysis of trafficking of ATP7A and ATP7B between the cell compartments, regulation of the trafficking by norepinephrine and other signaling molecules, intracellular measurements of coper transport activity, studies of the redox environment in live cells, and evaluation of tissue morphology with a quantitative analysis of cell densities. Studies in live cells would allow more sophisticated kinetic measurements as well as evaluation of spatial aspects of DBH secretion, which has not been done previously. The ability to re-use several components of the Pascal microscope, which is no longer supported by the manufacturer, resulted in a significant discount in the price of the microscope, despite addition of the live-cell imaging option, which is highly beneficial for the proposed research.

项目总结 LSM800工具将大量用于父母补助金的所有具体目标。这些研究 这将在显微镜下进行，包括分析ATP7A和ATP7B的贩运 在细胞隔间之间，去甲肾上腺素和其他信号对运输的调节 分子，细胞内铜转运活性的测量，氧化还原的研究 活细胞中的环境，以及用细胞定量分析评价组织形态 密度。在活细胞中的研究将允许更复杂的动力学测量以及 对胸径分泌的空间方面的评估，这是以前没有做过的。一种能力 重复使用PASCAL显微镜的几个部件，这些部件不再受 制造商，导致显微镜的价格大幅折扣，尽管增加了 活体细胞成像选项，这对拟议的研究非常有利。