Molecular mechanism of ATP-dependent copper transporters

ATP依赖性铜转运蛋白的分子机制

• 批准号: 8040090
• 负责人: SVETLANA LUTSENKO
• 金额: $ 45万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8040090
• 关键词: ATP phosphohydrolase; Affect; Binding; Biochemical; Biological; Biological Assay; Blood; Caco-2 Cells; Cells; Cerebrospinal Fluid; Cisplatin; Code; Confocal Microscopy; Copper; Coupled; Cytosol; Development; Diagnostic; Disease; Electron Transport; Embryo; Enzymes; Equilibrium; Event; Excision; Financial compensation; Genes; Genetic; Goals; Growth and Development function; Hepatolenticular Degeneration; Homeostasis; Human; Hypoxia; In Vitro; Label; Link; Liquid substance; Mediating; Menkes Kinky Hair Syndrome; Metabolic; Metabolism; Metals; Milk; Molecular; Molecular Chaperones; Molecular Conformation; Mutate; Mutation; N-terminal; Organism; Oxidation-Reduction; Pathway interactions; Pharmaceutical Preparations; Phosphorylation; Phosphotransferases; Physiology; Play; Process; Property; Proteins; Regulation; Relative (related person); Reporting; Research; Resistance; Role; Series; Site-Directed Mutagenesis; Solid Neoplasm; System; Testing; Tetanus Helper Peptide; Up-Regulation; Variant; Wilson disease protein; base; cardiogenesis; copper-transporting ATPase; design; disease-causing mutation; hypocupremia; improved; insight; iron metabolism; mutant; overexpression; oxidation; preference; research study; sensor; tool; trafficking

DESCRIPTION (provided by applicant): The major goal of this project is to understand the molecular and cellular mechanisms that regulate the transport and distribution of copper in human cells. Copper is essential for normal growth and development of human organisms. Copper misbalance results in severe multi-system disorders exemplified by Menkes disease and Wilson's disease. The genes affected in Menkes disease and Wilson's disease code for the copper-transporting ATPases ATP7A and ATP7B, respectively. The copper-transporting ATPases play a central role in human copper homeostasis by delivering copper to the copper-dependent enzymes as well as exporting excess copper from cells. The activity of copper- ATPases is tightly regulated at the molecular and cellular level. The molecular mechanism of this regulation is poorly understood and will be elucidated in the proposed series of experiments, which have four specific aims. Aim 1 will compare the regulation of ATP7A and ATP7B by the copper chaperone Atox1. Aim 2 will determine the role of oxidation/reduction for the copper-ATPase activity. Aim 3 is designed to understand the role of a kinase-mediated phosphorylation in modulating the Cu- ATPases activity and intracellular localization. Aim 4 will characterize a series of Wilson's disease causing mutations to dissect their molecular and cellular consequences. The results will clarify the relative contribution of each copper-ATPase to cellular copper balance as well as the biochemical basis for incomplete compensation of one copper-ATPase by the other in disease. New tools for the analysis of copper transporters in cells will be developed. The results of this research will contribute to the development of better diagnostics and treatments for human disorders of copper metabolism. PUBLIC HEALTH RELEVANCE: The project focuses on understanding the function and regulation of human copper transporters ATP7A and ATP7B, associated with Menkes disease and Wilson's disease, respectively. The studies will identify factors that modulate the activity of the copper transporters in cells and characterize the consequences of known disease- causing mutations. The results will contribute to improved diagnostic and treatment of human disorders of copper metabolism.

描述（由申请人提供）：本项目的主要目标是了解调节铜在人体细胞中运输和分布的分子和细胞机制。铜是人体正常生长和发育所必需的。铜失衡导致严重的多系统疾病，例如门克斯病和威尔逊病。门克斯病和威尔逊病中受影响的基因分别编码铜转运ATP酶ATP 7A和ATP 7 B。铜转运ATP酶通过将铜输送到铜依赖酶以及从细胞中输出过量的铜，在人体铜稳态中发挥核心作用。铜-ATP酶的活性在分子和细胞水平上受到严格的调节.这种调节的分子机制知之甚少，将在拟议的一系列实验中阐明，这些实验有四个具体目标。目的1将比较铜伴侣Atox 1对ATP 7A和ATP 7 B的调节。目的2将确定氧化/还原对铜-ATP酶活性的作用。目的3是了解激酶介导的磷酸化在调节Cu-ATP酶活性和细胞内定位中的作用。目的4将描述一系列导致威尔逊病突变的特征，以剖析其分子和细胞后果。这些结果将阐明每个铜-ATP酶对细胞铜平衡的相对贡献，以及疾病中一个铜-ATP酶不完全补偿另一个铜-ATP酶的生化基础。将开发用于分析细胞中铜转运蛋白的新工具。这项研究的结果将有助于开发更好的诊断和治疗人类铜代谢紊乱。 公共卫生关系：该项目的重点是了解人类铜转运蛋白ATP 7A和ATP 7 B的功能和调节，分别与门克斯病和威尔逊病相关。 这些研究将确定调节细胞中铜转运蛋白活性的因素，并描述已知致病突变的后果。 研究结果将有助于改善人类铜代谢紊乱的诊断和治疗。