Molecular Structure of Chromium-DNA Adducts
铬-DNA 加合物的分子结构
基本信息
- 批准号:10358312
- 负责人:
- 金额:$ 43.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-07-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:Amino AcidsAnimalsAntioxidantsAreaBase CompositionBase PairingBase SequenceBindingBinding SitesBiochemicalBiologicalCarcinogensCategoriesCell Culture TechniquesCellsChemistryChromatesChromiumComplexCultured CellsDNADNA AdductsDNA DamageDNA SequenceDataExposure toGoalsHumanIndustrializationInhalationKnowledgeLaboratoriesLeadLesionLocationMagnetismMalignant NeoplasmsMismatch RepairMolecularMolecular StructureMutagenesisMutagensMutationNatureOligonucleotidesOralOxidation-ReductionPathway interactionsPeptidesPolymersPopulationProcessProteinsRoleSiteStructureSulfhydryl CompoundsSystemTechniquesTestingWorkadductascorbatebasecalf thymus DNAcarcinogenesiscarcinogenicitychemical propertychromium hexavalent ioncrosslinkdesignexperimental studygenotoxicityinsightplasmid DNApreventrepairedsmall moleculetumorigenesisvirtual
项目摘要
Chromium(VI) complexes are potent mutagens and carcinogens when inhaled, while the
potential of these complexes to generate similar effects when taken orally is an area of active
debate. The exact mechanism(s) of action of this activity is unknown, but potential mechanisms
can be grouped into two categories. The first is mechanisms associated with the redox
chemistry during the reduction of Cr(VI) ultimately to Cr(III). In the 20 years, significant progress
has been made in characterizing alterations to DNA results from these redox processes. The
second mechanism is based on the generated Cr(III) binding to DNA to form binary and ternary
complexes, which ultimately give may arise to the mutagenic and carcinogenic effects.
Unfortunately, while these Cr‐DNA complexes have been studied intensely the last circa 15
years, virtually no data on the molecular level structure of these Cr(III)‐DNA complexes exists.
Such knowledge is essential to understanding and determining the potential of these complexes
to lead to deleterious effects. Because of the unique magnetic and chemical properties of Cr(III)
complexes, characterization of Cr(III)‐biomolecules is not trivial, requiring special expertise.
Additionally, previous studies have used plasmid DNA, DNA polymers, calf thymus DNA, or DNA
isolated from cultured cells, which because of their size and complexity present numerous
potential Cr‐binding sites with a wide range of binding constants. What is required to determine
the preferential sites for Cr‐binding and to characterize the structure of these sites is the use of
DNA oligomers significantly smaller in size whose base sequences can be carefully designed and
which can readily be synthesized in appreciable quantities and whose Cr(III) complexes are
readily amendable to characterization by spectroscopic and magnetic techniques. Our long term
goal is to characterize the binding of chromium(III) to DNA at a molecular level and relate the
structures to the genotoxicity and carcinogenicity of Cr(VI).
六价铬(VI)络合物吸入时具有很强的致突变性和致癌性,而
这些复合体在口服时产生类似效果的潜力是一个活跃的领域
辩论。这一活动的确切作用机制(S)尚不清楚,但可能的机制
可以分为两类。第一个是与氧化还原有关的机制。
铬(VI)最终还原为铬(III)的化学过程。在过去的20年里,取得了重大进展
已经在表征这些氧化还原过程对DNA结果的改变方面取得了进展。这个
第二种机制是基于生成的铬(III)与DNA结合形成二元和三元
最终可能会产生致突变和致癌作用。
不幸的是,虽然这些铬-DNA复合体在过去的15年里被密集地研究了
多年来,几乎没有关于这些铬(III)-DNA络合物的分子级结构的数据。
这些知识对于理解和确定这些络合物的潜力是必不可少的。
导致有害的影响。由于铬(III)独特的磁性和化学性质
对于铬(III)-生物分子的表征,不是微不足道的,需要特殊的专业知识。
此外,以前的研究使用了质粒DNA、DNA聚合物、小牛胸腺DNA或DNA
从培养的细胞中分离出来的,由于它们的大小和复杂性,呈现出许多
具有大范围结合常数的潜在铬结合部位。需要什么才能确定
铬结合的优先位置和表征这些位置的结构是使用
DNA低聚物的大小显著小于其碱基序列可以仔细设计和
它可以很容易地合成,其铬(III)络合物是
易于通过光谱和磁性技术对其进行表征。我们的长期计划
目的是在分子水平上表征铬(III)与DNA的结合,并与
结构对铬(VI)的遗传毒性和致癌作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JOHN Bertram VINCENT其他文献
JOHN Bertram VINCENT的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JOHN Bertram VINCENT', 18)}}的其他基金
Elucidating the properties of low-molecular-weight chromuim-binding substance
阐明低分子量铬结合物质的特性
- 批准号:
7078835 - 财政年份:2006
- 资助金额:
$ 43.45万 - 项目类别:
Elucidating the properties of low-molecular-weight chromuim-binding substance
阐明低分子量铬结合物质的特性
- 批准号:
7268070 - 财政年份:2006
- 资助金额:
$ 43.45万 - 项目类别:
Elucidating the Biochemistry of Chromium (III)
阐明铬 (III) 的生物化学
- 批准号:
6651872 - 财政年份:2002
- 资助金额:
$ 43.45万 - 项目类别:
CHARACTERIZATION OF ACTIVE SITES OF PURPLE PHOSPHATASES
紫色磷酸酶活性位点的表征
- 批准号:
3044663 - 财政年份:1991
- 资助金额:
$ 43.45万 - 项目类别:
CHARACTERIZATION OF ACTIVE SITES OF PURPLE PHOSPHATASES
紫色磷酸酶活性位点的表征
- 批准号:
3044662 - 财政年份:1990
- 资助金额:
$ 43.45万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 43.45万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 43.45万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 43.45万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 43.45万 - 项目类别:
Discovery Early Career Researcher Award
Zootropolis: Multi-species archaeological, ecological and historical approaches to animals in Medieval urban Scotland
Zootropolis:苏格兰中世纪城市动物的多物种考古、生态和历史方法
- 批准号:
2889694 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Studentship
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Training Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Continuing Grant
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 43.45万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)














{{item.name}}会员




