MHC Genetic Typing Core

项目概要-核心3：MHC基因分型核心 MHC和遗传分型核心的目标是支持遗传表征和育种 SPF菌落的战略，重点是优化艾滋病相关研究的殖民地。该芯体的 三个目标建立在核心的现有优势，并纳入新的方法， 对殖民地进行严格的遗传鉴定。 在目标1中，对艾滋病研究者很重要的MHC I类等位基因将使用高分辨率基因分型技术进行基因分型。 与威斯康星州NPRC的Wiseman博士合作进行测序。185的基因型 每年都会有动物出现。 在目标2中，基因型测序（GbS）和全基因组测序（WGS）将用于提供 a）一个更强大的血统信息标记（AIM）集，定义印度和中国血统 全基因组和特定基因座的信息，B）用于验证谱系的亲子关系信息， 调查群体起源，c）基因型信息，以识别携带 与艾滋病相关研究和影响传染病相关的已知和潜在的新等位基因 发病机理和疫苗有效性，以及d）开发共享遗传数据的方法 研究社区使用基于Web的用户界面，允许访问LabKey管理的 数据 在目标3中，目标1和2的遗传数据将用于最大限度地提高殖民地的遗传多样性， 保持足够数量的动物具有特异性MHC I类等位基因和SIV/HIV祖先 相关研究。核心3将与核心1和SPF监督委员会合作， 使用来自菌落1和2的动物的组。

PROJECT SUMMARY – Core 3: MHC Genetic Typing Core The objectives of the MHC and Genetic Typing Core are to support the genetic characterization and breeding strategy of the SPF Colony, with a focus towards optimizing the colony for AIDS-related research. The Core's three aims build upon the current strengths of the Core and incorporate new approaches towards more rigorous genetic characterization of the colony. In Aim 1, MHC Class I alleles, important to AIDS investigators, will be genotyped using high resolution sequencing in collaboration with Dr. Wiseman at the Wisconsin NPRC. The genotypes on 185 animals will be obtained each year. In Aim 2, both genotype-by-sequencing (GbS) and whole genome sequencing (WGS) will be used to provide a) a more robust Ancestry Informative Markers (AIMs) set defining Indian and Chinese ancestry information both genome-wide and at specific loci, b) parentage information to verify pedigrees and investigate colony origins, c) genotypic information to allow for the identification of animals carrying known and potentially new alleles relevant for AIDS-related research and impacting infectious disease pathogenesis and vaccine effectiveness, and d) development of a means to share the genetic data with the research community using a web-based user interface allowing access to LabKey-managed data. In Aim 3, genetic data from Aims 1 and 2 will be used to maximize genetic diversity of the colony, while maintaining sufficient numbers of animals with specific MHC Class I alleles and ancestry for SIV/HIV related studies. Core 3 will work with Core 1 and the SPF Oversight Committee to assemble breeding groups using animals derived from the both Colonies 1 and 2.