NE-CAT: A Resource for Advanced Macromolecular Crystallography
NE-CAT:高级高分子晶体学资源
基本信息
- 批准号:10505648
- 负责人:
- 金额:$ 379.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-04-01 至 2028-03-31
- 项目状态:未结题
- 来源:
- 关键词:20 year oldAdvisory CommitteesAgingAreaAutomatic Data ProcessingBiologicalBiological ProcessBiomedical ResearchCharacteristicsCommunitiesComputer softwareCrystallographyData AnalysesData CollectionDedicationsDevelopmentEducation and OutreachEducational workshopEnsureFundingGoalsGroupingHealthcareIndividualInfrastructureInstitutionLogisticsMainstreamingMaintenanceManuscriptsModificationOpticsPaperPersonsPharmacologic SubstancePhotonsProductivityProfessional OrganizationsPublicationsPublishingRecruitment ActivityResearchResearch PersonnelResearch Project GrantsResourcesRoboticsRoentgen RaysRunningSamplingScheduleSiteSourceStructureSynchrotronsSystemTechniquesTechnologyTimeTrainingTraining ActivityTraining ProgramsUnited States National Institutes of HealthUniversitiesWorkbeamlinebiological systemscomputerized data processingdata structuredesigndetectorexperienceimprovedmacromoleculemeetingsmemberoperationoutreachoutreach programprogramsrepairedthree dimensional structurethree-dimensional visualizationweb based interfaceweb site
项目摘要
The main goal of NE-CAT is to provide user access to state-of-the-art synchrotron beamlines for
macromolecular crystallography. NE-CAT began planning and design for two beamlines in 2001. Beamline
24-ID-C became available to Advanced Photon Source users in 2006 and beamline 24-ID-E became
available in 2007. During the ensuing years, beamline improvements have resulted in stable, small X-ray
beams ideally suited for challenging problems in macromolecular crystallography. Each beamline is
equipped with a high capacity sample automounter and a microdiffractometer. Beamline 24-ID-C features
an EIGER2 X 16M pixel array detector, and 24-ID-E features an EIGER X 16M. NE-CAT provides
automated software for data collection and analysis (RAPD) and a web-based interface for remote data
collection. We will continue to provide routine maintenance to ensure efficient and reliable data collection,
and we propose an upgrade program to maintain state-of-the-art capabilities over the five years covered by
this application. This period includes 12 months without X-rays required for the APS upgrade project. We
will use this superb opportunity to rebuild a nearly 20-year-old system to be leaders in the field for another
20 years. NE-CAT has an experienced and dedicated scientific staff who enable access to the beamlines.
The staff is available or on call 24/7 to assist users with data collection and processing needs. This highly
regarded staff is a major strength of NE-CAT. NE-CAT has an outstanding record of reliability with technical
problems rarely interfering with user access. NE-CAT is directed by Dr. Steven Ealick, who has more that
30 years of experience overseeing the operation of synchrotron facilities for macromolecular
crystallography. The technical group, led by Deputy Director Dr. Malcolm Capel, and the operations group,
led by Associate Director Dr. Frank Murphy work closely together to ensure that the needs of the user
community are met. Together, these three individuals make up the NE-CAT management team. NE-CAT
beam time is in high demand, and each run all available beam time is allocated to users. NE-CAT typically
hosts more than 1500 users per year from about 160 unique user groups, either on site or remotely. NE-
CAT users published about 200 papers during the last year and are projected to publish more than 190 in
2021. NE-CAT’s record of productivity places it among the top of worldwide facilities for macromolecular
crystallography. NE-CAT operates an extensive user training program adaptable to first time users,
advanced users with technically challenging problems, and everyone in between. A website is available to
provide all information needed by new or current users. NE-CAT participates in many training and outreach
activities including organization of an annual workshop and participation in professional society meetings.
The staff works closely with user groups and are often invited to coauthor manuscripts resulting from user
support and training.
NE-CAT的主要目标是为用户提供对最先进的同步加速器光束线的访问
大分子结晶学。NE-CAT于2001年开始规划和设计两条光束线。光束线
24-ID-C在2006年向高级光子源用户提供,光束线24-ID-E成为
2007年上市。在接下来的几年里,光束线的改进产生了稳定的、小的X射线
光束非常适合于大分子结晶学中具有挑战性的问题。每条光束线都是
配备大容量样品自动安装机和显微衍射仪。Beamline 24-ID-C功能
EIGER2 X 16M像素阵列探测器,24-ID-E采用Eiger X 16M。Ne-CAT提供
自动化数据收集和分析软件(RAPD)和基于Web的远程数据界面
收集。我们将继续提供例行维护,以确保有效和可靠的数据收集,
我们提出了一项升级计划,以在五年内保持最先进的能力,
这个应用程序。这段时间包括12个月没有进行APS升级项目所需的X光检查。我们
将利用这个绝佳的机会重建一个有近20年历史的系统,成为该领域的领导者
20年了。NE-CAT拥有经验丰富和敬业的科学工作人员,他们能够访问光束线。
工作人员全天候或随叫随到,以帮助用户满足数据收集和处理需求。如此之高
尊重员工是NE-CAT的一大优势。NE-CAT拥有卓越的可靠性记录和技术
很少出现干扰用户访问的问题。NE-CAT由史蒂文·伊利克博士执导,他有更多
30年监督大分子同步加速器设备运行的经验
结晶学。由副局长马尔科姆·卡佩尔博士领导的技术小组和运营小组,
在副主任Frank Murphy博士的带领下,紧密合作,确保用户的需求
社区是相遇的。这三个人一起组成了NE-CAT管理团队。NE-CAT
射束时间需求很高,每次运行都将所有可用的射束时间分配给用户。Ne-cat典型的
每年托管来自现场或远程的约160个独立用户组的1500多名用户。不会的-
CAT用户在去年发表了大约200篇论文,预计将在
2021年。NE-CAT的生产率记录使其跻身于全球最先进的高分子设备之列
结晶学。NE-CAT运行适用于第一次用户的广泛用户培训计划,
具有技术挑战性问题的高级用户,以及介于两者之间的每个人。一个网站可以用来
提供新用户或现有用户所需的所有信息。Ne-CAT参加了许多培训和外展活动
活动包括组织年度讲习班和参加专业协会会议。
工作人员与用户群体密切合作,经常被邀请共同撰写用户生成的稿件
支持和培训。
项目成果
期刊论文数量(312)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Crystal structure of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshifting pseudoknot.
- DOI:10.1261/rna.078825.121
- 发表时间:2022-03
- 期刊:
- 影响因子:0
- 作者:Jones CP;Ferré-D'Amaré AR
- 通讯作者:Ferré-D'Amaré AR
Bipartite binding of the N terminus of Skp2 to cyclin A.
- DOI:10.1016/j.str.2021.04.011
- 发表时间:2021-09-02
- 期刊:
- 影响因子:0
- 作者:Kelso S;Orlicky S;Beenstock J;Ceccarelli DF;Kurinov I;Gish G;Sicheri F
- 通讯作者:Sicheri F
Post-Catalytic Complexes with Emtricitabine or Stavudine and HIV-1 Reverse Transcriptase Reveal New Mechanistic Insights for Nucleotide Incorporation and Drug Resistance.
催化后复合物与Emtritebine或Stavudine和HIV-1逆转录酶揭示了核苷酸掺入和耐药性的新机械见解。
- DOI:10.3390/molecules25204868
- 发表时间:2020-10-21
- 期刊:
- 影响因子:0
- 作者:Bertoletti N;Chan AH;Schinazi RF;Anderson KS
- 通讯作者:Anderson KS
Correction to "A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain".
对“宏结构域的荧光偏振测定有利于识别 SARS-CoV-2 宏结构域的有效抑制剂”的更正。
- DOI:10.1021/acschembio.4c00159
- 发表时间:2024
- 期刊:
- 影响因子:4
- 作者:Anmangandla,Ananya;Jana,Sadhan;Peng,Kewen;Wallace,ShamarD;Bagde,SaketR;Drown,BryonS;Xu,Jiashu;Hergenrother,PaulJ;Fromme,JChristopher;Lin,Hening
- 通讯作者:Lin,Hening
Discovery and Structural Characterization of Small Molecule Binders of the Human CTLH E3 Ligase Subunit GID4.
- DOI:10.1021/acs.jmedchem.2c00509
- 发表时间:2022-10-13
- 期刊:
- 影响因子:7.3
- 作者:Chana, Chetan K.;Maisonneuve, Pierre;Posternak, Ganna;Grinberg, Nicolas G. A.;Poirson, Juline;Ona, Samara M.;Ceccarelli, Derek F.;Mader, Pavel;St-Cyr, Daniel J.;Pau, Victor;Kurinov, Igor;Tang, Xiaojing;Deng, Dongjing;Cui, Weiren;Su, Wenji;Kuai, Letian;Soll, Richard;Tyers, Mike;Rost, Hannes L.;Batey, Robert A.;Taipale, Mikko;Gingras, Anne-Claude;Sicheri, Frank
- 通讯作者:Sicheri, Frank
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{{ truncateString('Nozomi Ando', 18)}}的其他基金
Protein Allostery and Catalysis Beyond Bragg Diffraction
布拉格衍射之外的蛋白质变构和催化
- 批准号:
10677766 - 财政年份:2017
- 资助金额:
$ 379.29万 - 项目类别:
Protein Allostery and Catalysis Beyond Bragg Diffraction
布拉格衍射之外的蛋白质变构和催化
- 批准号:
10406516 - 财政年份:2017
- 资助金额:
$ 379.29万 - 项目类别:
Protein Allostery and Catalysis Beyond Bragg Diffraction
布拉格衍射之外的蛋白质变构和催化
- 批准号:
10806507 - 财政年份:2017
- 资助金额:
$ 379.29万 - 项目类别:
Protein Allostery and Catalysis Beyond Bragg Diffraction
布拉格衍射之外的蛋白质变构和催化
- 批准号:
10250499 - 财政年份:2017
- 资助金额:
$ 379.29万 - 项目类别:
Protein Allostery and Catalysis Beyond Bragg Diffraction
布拉格衍射之外的蛋白质变构和催化
- 批准号:
10798624 - 财政年份:2017
- 资助金额:
$ 379.29万 - 项目类别:
Structural Characterizations of Transient and Heterogeneous Protein Complexes
瞬时和异质蛋白质复合物的结构表征
- 批准号:
8417652 - 财政年份:2012
- 资助金额:
$ 379.29万 - 项目类别:
Structural Characterizations of Transient and Heterogeneous Protein Complexes
瞬时和异质蛋白质复合物的结构表征
- 批准号:
8895472 - 财政年份:2012
- 资助金额:
$ 379.29万 - 项目类别:
Structural Characterizations of Transient and Heterogeneous Protein Complexes
瞬时和异质蛋白质复合物的结构表征
- 批准号:
8225883 - 财政年份:2012
- 资助金额:
$ 379.29万 - 项目类别:
Structural Studies of Ribonucleotide Reductase Complexes
核糖核苷酸还原酶复合物的结构研究
- 批准号:
8013604 - 财政年份:2010
- 资助金额:
$ 379.29万 - 项目类别:
Structural Studies of Ribonucleotide Reductase Complexes
核糖核苷酸还原酶复合物的结构研究
- 批准号:
7806713 - 财政年份:2010
- 资助金额:
$ 379.29万 - 项目类别:
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