Tissue Engineering Plant-based Vascular Grafts
组织工程植物血管移植物
基本信息
- 批准号:10439063
- 负责人:
- 金额:$ 39.54万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-08-01 至 2025-07-31
- 项目状态:未结题
- 来源:
- 关键词:AffectAmericanAnimalsArteriesAutologousBiochemicalBiological AssayBiomedical EngineeringBioreactorsBlood VesselsBlood flowBypassCaliberCareer ChoiceCell AdhesionCell-Matrix JunctionCellsCelluloseCessation of lifeClinicalCoronary heart diseaseCustomDNADepositionDetergentsEgtazic AcidEndothelial CellsEndotheliumEngraftmentEnvironmentExcisionExtracellular MatrixExtracellular Matrix ProteinsFeasibility StudiesFluorescence MicroscopyFutureGoalsHistologyHyperplasiaImage AnalysisImmune responseImplantLaboratoriesLegal patentLiquid substanceMechanicsMesenchymal Stem CellsMethodsModelingMonitorMuscle TonusMyocardial InfarctionOperative Surgical ProceduresPatientsPerformancePerfusionPeripheral arterial diseasePlant LeavesPlantsPlayPropertyProteinsPumpRattusResearchResearch PersonnelRiskRoleSaphenous VeinScanning Electron MicroscopySmooth MuscleSmooth Muscle MyocytesSpecialistSurgeonSuspensionsSyringesTestingThinnessThrombosisTimeTissue EngineeringTissuesTransplantationTrypsinUltrasonographyUnited StatesUniversitiesValidationVascular GraftVascular Smooth MuscleVascular blood supplyWorkanimal tissuebasecommon treatmentconditioningexperimental studyfluorescence imaginghands on researchimplantationimprovedin vivoinsightmechanical propertiesocular surfacepreconditioningpressurepreventpublic health relevancereconstructionrecruitrepairedscaffoldshear stressskillsstem cell differentiationsuccessthrombogenesisundergraduate studentvascular tissue engineering
项目摘要
Abstract
Coronary heart disease and peripheral artery disease affects millions of Americans each year, and is
often treated with bypass surgery to reroute the blood supply around a blocked artery. However, patients do not
always have a saphenous vein suitable for an arterial bypass graft. The structural similarities between animals
and plants inspired a more recent strategy of decellularizing plants in order to generate perfusable scaffolds.
Unlike animal tissue, plants are primarily composed of cellulose which can offer a promising, nonthrombogenic
alternative capable of promoting cell attachment and redirecting blood flow. The immediate goal of this project
is to succeed in tissue engineering and testing a patent, nonthrombogenic vessel for engraftment that mimics
the mechanical and structural properties of native vessels. We hypothesize that the mechanical and biochemical
microenvironment provided by the decellularized plant leaves will promote initial endothelial cell and vascular
smooth muscle cell adhesion, maintain vessel patency, and that the addition of fluid shear stress preconditioning
will promote long-term endothelialization of the scaffold and minimize early graft occlusions in vivo. Based on
our preliminary pressure tests and cell adhesion assays showing that decellularized plant leaves maintain their
mechanical properties and promote endothelial cell adhesion, we believe this work will provide a useful method
of decellularizing plants and pre-conditioning of cells into a natural scaffold capable of successful engraftment.
The proposed project will also enhance the research environment at Hofstra University by allowing
undergraduate students to plan, execute and perform analysis of authentic hands-on research. This would allow
them to acquire a broad range of skills in biomedical engineering that they would otherwise not have access to
and is expected to have a significant impact on their future studies and career choices.
摘要
冠心病和外周动脉疾病每年影响数百万美国人,
通常通过搭桥手术来治疗,以改变阻塞动脉周围的血液供应。然而,患者不
总是有一个隐静脉适合动脉旁路移植术。动物之间的结构相似性
植物启发了最近的一种策略,即使植物去细胞化以产生可灌注的支架。
与动物组织不同,植物主要由纤维素组成,纤维素可以提供有前途的,非血栓形成的
另一种选择是能够促进细胞附着和重新定向血流。这个项目的近期目标
是成功地进行组织工程,并测试一种专利的、无血栓形成的血管,
天然血管的机械和结构特性。我们假设机械和生物化学
由脱细胞植物叶提供的微环境将促进初始内皮细胞和血管形成。
平滑肌细胞粘附,维持血管通畅,并认为加流体切应力预处理
将促进支架的长期内皮化并使体内早期移植物闭塞最小化。基于
我们初步的压力测试和细胞粘附试验表明,脱细胞植物叶片保持其
力学性能和促进内皮细胞粘附,我们相信这项工作将提供一个有用的方法
将植物脱细胞并将细胞预处理成能够成功植入的天然支架。
拟议的项目还将改善霍夫斯特拉大学的研究环境,
本科生计划,执行和执行真实的动手研究分析。这将允许
他们获得了广泛的技能,在生物医学工程,他们否则将无法获得
并将对他们未来的学业和职业选择产生重大影响。
项目成果
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