Tissue Engineering Plant-based Vascular Grafts

组织工程植物血管移植物

• 批准号: 10439063
• 负责人: Nicholas J. Merna
• 金额: $ 39.54万
• 依托单位: HOFSTRA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439063
• 关键词: Affect; American; Animals; Arteries; Autologous; Biochemical; Biological Assay; Biomedical Engineering; Bioreactors; Blood Vessels; Blood flow; Bypass; Caliber; Career Choice; Cell Adhesion; Cell-Matrix Junction; Cells; Cellulose; Cessation of life; Clinical; Coronary heart disease; Custom; DNA; Deposition; Detergents; Egtazic Acid; Endothelial Cells; Endothelium; Engraftment; Environment; Excision; Extracellular Matrix; Extracellular Matrix Proteins; Feasibility Studies; Fluorescence Microscopy; Future; Goals; Histology; Hyperplasia; Image Analysis; Immune response; Implant; Laboratories; Legal patent; Liquid substance; Mechanics; Mesenchymal Stem Cells; Methods; Modeling; Monitor; Muscle Tonus; Myocardial Infarction; Operative Surgical Procedures; Patients; Performance; Perfusion; Peripheral arterial disease; Plant Leaves; Plants; Play; Property; Proteins; Pump; Rattus; Research; Research Personnel; Risk; Role; Saphenous Vein; Scanning Electron Microscopy; Smooth Muscle; Smooth Muscle Myocytes; Specialist; Surgeon; Suspensions; Syringes; Testing; Thinness; Thrombosis; Time; Tissue Engineering; Tissues; Transplantation; Trypsin; Ultrasonography; United States; Universities; Validation; Vascular Graft; Vascular Smooth Muscle; Vascular blood supply; Work; animal tissue; base; common treatment; conditioning; experimental study; fluorescence imaging; hands on research; implantation; improved; in vivo; insight; mechanical properties; ocular surface; preconditioning; pressure; prevent; public health relevance; reconstruction; recruit; repaired; scaffold; shear stress; skills; stem cell differentiation; success; thrombogenesis; undergraduate student; vascular tissue engineering

Abstract Coronary heart disease and peripheral artery disease affects millions of Americans each year, and is often treated with bypass surgery to reroute the blood supply around a blocked artery. However, patients do not always have a saphenous vein suitable for an arterial bypass graft. The structural similarities between animals and plants inspired a more recent strategy of decellularizing plants in order to generate perfusable scaffolds. Unlike animal tissue, plants are primarily composed of cellulose which can offer a promising, nonthrombogenic alternative capable of promoting cell attachment and redirecting blood flow. The immediate goal of this project is to succeed in tissue engineering and testing a patent, nonthrombogenic vessel for engraftment that mimics the mechanical and structural properties of native vessels. We hypothesize that the mechanical and biochemical microenvironment provided by the decellularized plant leaves will promote initial endothelial cell and vascular smooth muscle cell adhesion, maintain vessel patency, and that the addition of fluid shear stress preconditioning will promote long-term endothelialization of the scaffold and minimize early graft occlusions in vivo. Based on our preliminary pressure tests and cell adhesion assays showing that decellularized plant leaves maintain their mechanical properties and promote endothelial cell adhesion, we believe this work will provide a useful method of decellularizing plants and pre-conditioning of cells into a natural scaffold capable of successful engraftment. The proposed project will also enhance the research environment at Hofstra University by allowing undergraduate students to plan, execute and perform analysis of authentic hands-on research. This would allow them to acquire a broad range of skills in biomedical engineering that they would otherwise not have access to and is expected to have a significant impact on their future studies and career choices.

摘要 冠心病和外周动脉疾病每年影响数百万美国人， 通常通过搭桥手术来治疗，以改变阻塞动脉周围的血液供应。然而，患者不 总是有一个隐静脉适合动脉旁路移植术。动物之间的结构相似性 植物启发了最近的一种策略，即使植物去细胞化以产生可灌注的支架。 与动物组织不同，植物主要由纤维素组成，纤维素可以提供有前途的，非血栓形成的 另一种选择是能够促进细胞附着和重新定向血流。这个项目的近期目标 是成功地进行组织工程，并测试一种专利的、无血栓形成的血管， 天然血管的机械和结构特性。我们假设机械和生物化学 由脱细胞植物叶提供的微环境将促进初始内皮细胞和血管形成。 平滑肌细胞粘附，维持血管通畅，并认为加流体切应力预处理 将促进支架的长期内皮化并使体内早期移植物闭塞最小化。基于 我们初步的压力测试和细胞粘附试验表明，脱细胞植物叶片保持其 力学性能和促进内皮细胞粘附，我们相信这项工作将提供一个有用的方法 将植物脱细胞并将细胞预处理成能够成功植入的天然支架。 拟议的项目还将改善霍夫斯特拉大学的研究环境， 本科生计划，执行和执行真实的动手研究分析。这将允许 他们获得了广泛的技能，在生物医学工程，他们否则将无法获得 并将对他们未来的学业和职业选择产生重大影响。