Tissue Engineering Plant-based Vascular Grafts

组织工程植物血管移植物

• 批准号: 10439063
• 负责人: Nicholas J. Merna
• 金额: $ 39.54万
• 依托单位: HOFSTRA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439063
• 关键词: Affect; American; Animals; Arteries; Autologous; Biochemical; Biological Assay; Biomedical Engineering; Bioreactors; Blood Vessels; Blood flow; Bypass; Caliber; Career Choice; Cell Adhesion; Cell-Matrix Junction; Cells; Cellulose; Cessation of life; Clinical; Coronary heart disease; Custom; DNA; Deposition; Detergents; Egtazic Acid; Endothelial Cells; Endothelium; Engraftment; Environment; Excision; Extracellular Matrix; Extracellular Matrix Proteins; Feasibility Studies; Fluorescence Microscopy; Future; Goals; Histology; Hyperplasia; Image Analysis; Immune response; Implant; Laboratories; Legal patent; Liquid substance; Mechanics; Mesenchymal Stem Cells; Methods; Modeling; Monitor; Muscle Tonus; Myocardial Infarction; Operative Surgical Procedures; Patients; Performance; Perfusion; Peripheral arterial disease; Plant Leaves; Plants; Play; Property; Proteins; Pump; Rattus; Research; Research Personnel; Risk; Role; Saphenous Vein; Scanning Electron Microscopy; Smooth Muscle; Smooth Muscle Myocytes; Specialist; Surgeon; Suspensions; Syringes; Testing; Thinness; Thrombosis; Time; Tissue Engineering; Tissues; Transplantation; Trypsin; Ultrasonography; United States; Universities; Validation; Vascular Graft; Vascular Smooth Muscle; Vascular blood supply; Work; animal tissue; base; common treatment; conditioning; experimental study; fluorescence imaging; hands on research; implantation; improved; in vivo; insight; mechanical properties; ocular surface; preconditioning; pressure; prevent; public health relevance; reconstruction; recruit; repaired; scaffold; shear stress; skills; stem cell differentiation; success; thrombogenesis; undergraduate student; vascular tissue engineering

Abstract Coronary heart disease and peripheral artery disease affects millions of Americans each year, and is often treated with bypass surgery to reroute the blood supply around a blocked artery. However, patients do not always have a saphenous vein suitable for an arterial bypass graft. The structural similarities between animals and plants inspired a more recent strategy of decellularizing plants in order to generate perfusable scaffolds. Unlike animal tissue, plants are primarily composed of cellulose which can offer a promising, nonthrombogenic alternative capable of promoting cell attachment and redirecting blood flow. The immediate goal of this project is to succeed in tissue engineering and testing a patent, nonthrombogenic vessel for engraftment that mimics the mechanical and structural properties of native vessels. We hypothesize that the mechanical and biochemical microenvironment provided by the decellularized plant leaves will promote initial endothelial cell and vascular smooth muscle cell adhesion, maintain vessel patency, and that the addition of fluid shear stress preconditioning will promote long-term endothelialization of the scaffold and minimize early graft occlusions in vivo. Based on our preliminary pressure tests and cell adhesion assays showing that decellularized plant leaves maintain their mechanical properties and promote endothelial cell adhesion, we believe this work will provide a useful method of decellularizing plants and pre-conditioning of cells into a natural scaffold capable of successful engraftment. The proposed project will also enhance the research environment at Hofstra University by allowing undergraduate students to plan, execute and perform analysis of authentic hands-on research. This would allow them to acquire a broad range of skills in biomedical engineering that they would otherwise not have access to and is expected to have a significant impact on their future studies and career choices.

摘要