Tissue Engineering Plant-based Vascular Grafts

组织工程植物血管移植物

• 批准号: 10439063
• 负责人: Nicholas J. Merna
• 金额: $ 39.54万
• 依托单位: HOFSTRA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10439063
• 关键词: Affect; American; Animals; Arteries; Autologous; Biochemical; Biological Assay; Biomedical Engineering; Bioreactors; Blood Vessels; Blood flow; Bypass; Caliber; Career Choice; Cell Adhesion; Cell-Matrix Junction; Cells; Cellulose; Cessation of life; Clinical; Coronary heart disease; Custom; DNA; Deposition; Detergents; Egtazic Acid; Endothelial Cells; Endothelium; Engraftment; Environment; Excision; Extracellular Matrix; Extracellular Matrix Proteins; Feasibility Studies; Fluorescence Microscopy; Future; Goals; Histology; Hyperplasia; Image Analysis; Immune response; Implant; Laboratories; Legal patent; Liquid substance; Mechanics; Mesenchymal Stem Cells; Methods; Modeling; Monitor; Muscle Tonus; Myocardial Infarction; Operative Surgical Procedures; Patients; Performance; Perfusion; Peripheral arterial disease; Plant Leaves; Plants; Play; Property; Proteins; Pump; Rattus; Research; Research Personnel; Risk; Role; Saphenous Vein; Scanning Electron Microscopy; Smooth Muscle; Smooth Muscle Myocytes; Specialist; Surgeon; Suspensions; Syringes; Testing; Thinness; Thrombosis; Time; Tissue Engineering; Tissues; Transplantation; Trypsin; Ultrasonography; United States; Universities; Validation; Vascular Graft; Vascular Smooth Muscle; Vascular blood supply; Work; animal tissue; base; common treatment; conditioning; experimental study; fluorescence imaging; hands on research; implantation; improved; in vivo; insight; mechanical properties; ocular surface; preconditioning; pressure; prevent; public health relevance; reconstruction; recruit; repaired; scaffold; shear stress; skills; stem cell differentiation; success; thrombogenesis; undergraduate student; vascular tissue engineering

Abstract Coronary heart disease and peripheral artery disease affects millions of Americans each year, and is often treated with bypass surgery to reroute the blood supply around a blocked artery. However, patients do not always have a saphenous vein suitable for an arterial bypass graft. The structural similarities between animals and plants inspired a more recent strategy of decellularizing plants in order to generate perfusable scaffolds. Unlike animal tissue, plants are primarily composed of cellulose which can offer a promising, nonthrombogenic alternative capable of promoting cell attachment and redirecting blood flow. The immediate goal of this project is to succeed in tissue engineering and testing a patent, nonthrombogenic vessel for engraftment that mimics the mechanical and structural properties of native vessels. We hypothesize that the mechanical and biochemical microenvironment provided by the decellularized plant leaves will promote initial endothelial cell and vascular smooth muscle cell adhesion, maintain vessel patency, and that the addition of fluid shear stress preconditioning will promote long-term endothelialization of the scaffold and minimize early graft occlusions in vivo. Based on our preliminary pressure tests and cell adhesion assays showing that decellularized plant leaves maintain their mechanical properties and promote endothelial cell adhesion, we believe this work will provide a useful method of decellularizing plants and pre-conditioning of cells into a natural scaffold capable of successful engraftment. The proposed project will also enhance the research environment at Hofstra University by allowing undergraduate students to plan, execute and perform analysis of authentic hands-on research. This would allow them to acquire a broad range of skills in biomedical engineering that they would otherwise not have access to and is expected to have a significant impact on their future studies and career choices.

抽象的 冠心病和周围动脉疾病每年都会影响数百万的美国人，并且是 经常接受旁路手术治疗，以重新穿线周围的动脉周围的血液供应。但是，患者没有 始终具有适合动脉旁路移植物的隐性静脉。动物之间的结构相似性 植物启发了一种最新的脱皮植物策略，以产生灌注脚手架。 与动物组织不同，植物主要由纤维素组成，该纤维素可以提供有希望的，非组织性的 能够促进细胞附着并重定向血流的替代方法。该项目的直接目标 是在组织工程和测试专利，非紧密的容器中取得成功的植入 天然血管的机械和结构特性。我们假设机械和生化 脱细胞植物叶提供的微环境将促进初始内皮细胞和血管 平滑肌细胞粘附，保持容器的通畅，并添加流体剪切应力预处理 将促进脚手架的长期内皮化，并在体内最大程度地减少早期移植术。基于 我们的初步压力测试和细胞粘附测定法，表明脱细胞叶片保持 机械性能并促进内皮细胞粘附，我们认为这项工作将提供有用的方法 将脱细胞和细胞预先调节成能够成功植入的天然支架。 拟议的项目还将通过允许 本科生可以计划，执行和执行真实的动手研究分析。这将允许 他们要获得广泛的生物医学工程技能，否则他们将无法获得 并有望对他们的未来研究和职业选择产生重大影响。