MicroRNA Regulation of Phospholipid Homeostasis in Alzheimer's Disease Pathogenesis

MicroRNA 对阿尔茨海默病发病机制中磷脂稳态的调节

• 批准号: 10368318
• 负责人: Dongming Cai
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10368318
• 关键词: 3-Dimensional; Age-Months; Alleles; Alzheimer' s Disease; Alzheimer' s disease pathology; Animals; Anti-Inflammatory Agents; Antiinflammatory Effect; Astrocytes; Attenuated; Autopsy; Axon; Behavioral; Brain; Brain region; Chelating Agents; Chronic; Coculture Techniques; Cognitive; Cognitive deficits; Complex; Copper; Cuprizone; Data; Data Set; Defect; Demyelinations; Development; Disease; Disease Progression; Down-Regulation; Enzymes; Etiology; Exhibits; Experimental Models; Female; Functional disorder; Funding; Gene Expression; Genes; Goals; Hippocampus (Brain); Homeostasis; Human; Impairment; Inflammation; Inflammatory; Inflammatory Response; Injections; Knock-in; Knock-in Mouse; Ligands; Lipopolysaccharides; MicroRNAs; Microglia; Modeling; Molecular; Molecular Profiling; Morphology; Mus; Myelin; Nature; Neurons; Pathogenesis; Pathogenicity; Pathology; Pathway interactions; Phagocytes; Phenotype; Phosphatidylinositol 4,5-Diphosphate; Phospholipids; Pilot Projects; Regulation; Resolution; Role; SYNJ1 gene; Sampling; Senile Plaques; System; TREM2 gene; Testing; Time; Viral; apolipoprotein E-4; brain cell; cognitive performance; cohort; cytokine; dietary control; exosome; follow-up; genetic risk factor; in vivo; induced pluripotent stem cell; knock-down; male; motor deficit; mouse model; network models; neuroinflammation; novel; overexpression; prevent; protein expression; recruit; response; single-cell RNA sequencing; targeted treatment; tau Proteins; transcriptome sequencing; uptake; validation studies; vector

PROJECT SUMMARY APOE4 is the strongest genetic risk factor for sporadic AD with Ab-dependent and Ab-independent effects on disease pathogenesis. However, the molecular mechanisms underlying the pathogenic nature of APOE4 in AD are not fully elucidated. In previous funding period (07/01/2017-present), we have made significant progress toward understanding micro-RNA (miRNA) regulation of APOE4-induced brain phospholipid dysregulation in AD. We have uncovered a novel regulatory mechanism of miR-195 targeted at APOE4-associated cognitive deficits and lysosomal defects in AD. Notably, we identified miR-195 as a top miRNA candidate involved in the APOE- regulated brain phosphoinositol biphosphate (PIP2) pathway using human ROSMAP and mouse microarray data. Levels of miR-195 are significantly lower in APOE4+ human and mouse brains, and in human inducible pluripotent stem cells (iPSC)-derived neurons and astrocytes when compared to APOE4- counterparts. Over- expressing miR-195 reduces expression levels of its top target synaptojanin 1 (synj1), the brain PIP2 degrading enzyme. Elevating miR-195 ameliorates cognitive deficits and AD pathology in APOE4+ mice and rescues lysosomal defects in APOE4+ iPSC brain cells. Furthermore, our preliminary results support the role of miR-195 as an anti-inflammatory miRNA in regulating microglial function. Our single cell (sc)-RNA seq. analysis of E4FAD mouse brains with miR-195 over-expression suggests that miR-195 alters molecular signatures of microglia sub- clusters. APOE4+ microglia with lower miR-195 levels and higher synj1 expression at baseline, manifests with impaired phagocytic activities and lysosomal defects when compared to APOE3+ microglia. Down-regulation of synj1 or over-expression of miR-195 can rescue these phenotypes. Beside synj1, inflammatory genes pdcd4 and smad7 are predicted targets of miR-195 as well. Over-expression of miR-195 in microglia inhibits lipopolysaccharide (LPS)-induced increases in smad7 and pdcd4 expression, attenuates LPS-induced proinflammatory cytokine release and augments anti-inflammatory responses. In addition, exosomes derived from APOE4/4 astrocytes (ADEs) contain less miR-195 than those in APOE3/3 ADEs, and over-expression of miR-195 in APOE4/4 astrocytes increases miR-195 levels in ADEs which can attenuate LPS-induced pro- inflammatory cytokine release. Therefore, we hypothesize that miR-195 may exhibit anti-inflammatory effects through down-regulation of microglial synj1 to regulate lysosomal function, direct target at microglial inflammatory gene expression and responses, and modulation of neuro-inflammation and tau spread by exosomal miR-195. We propose to characterize the regulation of microglial function by miR-195 during AD pathogenesis in this renewal application. We will: 1) determine the impact of miR-195 on microglia function and APOE-regulated neuro-inflammation in AD in vivo (Aim 1) using cuprizone (CPZ)-induced inflammation in male and female EFAD mouse models (human ApoE4 knock-in at 5xFAD background); 2) to characterize the molecular mechanisms by which miR-195 regulates AD-associated neuro-inflammation (Aim 2) using microglial culture and 3-D co-culture system of mouse brain cells from EFAD and APOE KI mice, as well as from synj1-/-, APOE-/- and TREM2-/- mice (with manipulations of miR-195 levels); 3) to perform high resolution multiscale network modeling using scRNA- seq dataset from mouse brains (Aim 1) and RNA-seq and miR-seq datasets from microglia and 3-D co-culture system (Aim 2) to identify microglia-specific molecular signatures driven by miR-195; and 4) to validate identified microglial signature driven by miR-195 in postmortem human brain samples and investigate their correlation with the development of AD-associated neuro-inflammation during disease progression (Aim 2). The goals of this application aim to elucidate novel pathways and molecular signatures driven by miR-195 protective against APOE4-induced microglial dysfunction in AD pathogenesis, which will facilitate identification and development of a more personalized targeted therapeutic approach to AD-associated neuro-inflammation.

项目总结