PDB MANAGEMENT BY THE RESEARCH COLLABORATORY FOR STRUCTURAL BIOINFORMATICS

项目总结 结构生物信息学蛋白质数据库研究合作实验室(RCSB PDB)的任务是 维护独特的可查找、可访问、可互操作、可重复使用的三维(3D)生活数据资源 生物分子结构信息。PDB结构是生命的分子。关于3D结构的知识 生物大分子的形状，它们如何随时间演变，以及它们的功能对于 了解科学的关键领域，包括基础生物学；人类、动物的健康和疾病， 和植物；粮食和能源生产；以及其他与全球繁荣和环境有关的问题 可持续发展。结构数据对生物制药和生物技术公司同样重要，推动 新疗法、生物材料和医疗设备的发现。RCSB PDB服务跨越整个PDB数据 生命周期，包括沉积/生物修复、归档管理/访问、数据探索和 外展/教育。RCSB PDB归档管理/访问服务保护单个全球归档 &gt；144,000个生物大分子的3D结构，估计重置成本近140亿美元。 RCSB PDB沉积/生物固化和数据探索服务支持&gt；30,000个数据存储者和&gt；1 全球有一百万数据消费者。超过620,000名研究人员、教育工作者、学生和 公众使用我们屡获殊荣的外展/教育服务。 在2019年至2023年期间，RCSB PDB有三个具体目标。成功完成这些具体目标将 需要在云计算/存储方面进一步投资，大量软件工程，以及适度的 招聘更多人员，以应对本组织面临的三大挑战。 具体目标1：扩大RCSB和PDB的业务能力，以满足(1)新结构的预计增长； (Ii)平均结构规模和复杂性；(Iii)年数据下载量；以及(Iv)用户和用户 社区及其多样性。具体目标2：扩展RCSB和PDB的业务能力，以满足计划 结构数据和复杂性的增长来自使用两个快速进化的分子机器的研究 已经黯然失色的实验方法(系列飞秒X射线晶体学或SFX和3DEM) 我们的一些服务能力。具体目标3：实施沉积-验证-生物修复、归档和 用于来自结合3DEM的新兴结构确定方法的结构数据的在线交付/工具 和/或具有目前不支持的多种测量技术的大分子结晶学 PDB。这些综合/杂交方法结构是细胞/生物生物学研究的先锋。 继续访问PDB数据和RCSB PDB服务将推动在全球范围内发表创新研究 生物医学、药物发现和开发、专利申请和导致发现的创新 以及开发改变生活的生物制药产品和组建新的美国公司。

PROJECT SUMMARY The mission of the Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB) is to sustain a unique Findable-Accessible-Interoperable-Reusable living data resource of three-dimensional (3D) biomolecular structure information. PDB structures are the molecules of life. Knowledge of 3D structures (shapes) of biological macromolecules, how they evolved with time, and their functions are essential for understanding critical areas of science, including fundamental biology; health and disease of humans, animals, and plants; food and energy production; and other topics of concern to global prosperity and environmental sustainability. Structure data are equally important for biopharmaceutical and biotechnology companies, driving discovery of new therapeutics, biomaterials, and medical devices. RCSB PDB services span the entire PDB data lifecycle, encompassing Deposition/Biocuration, Archive Management/Access, Data Exploration, and Outreach/Education. RCSB PDB Archive Management/Access services safeguard the single global archive of >144,000 3D structures of biological macromolecules, with an estimated replacement cost of nearly $14 billion. RCSB PDB Deposition/Biocuration and Data Exploration services support >30,000 Data Depositors and >1 million Data Consumers worldwide. More than 620,000 researchers, educators, students, and members of the public use our award winning Outreach/Education Services. During 2019-2023, the RCSB PDB has three Specific Aims. Successful completion of these Specific Aims will entail further investments in cloud computing/storage, considerable software engineering, and modest recruitment of additional personnel to address three overarching challenges that confront the organization. Specific Aim 1: Scale RCSB PDB operational capabilities to meet projected growth in (i) new structures; (ii) average structure size and complexity; (iii) annual data download volume; and (iv) Users and User communities, and diversity thereof. Specific Aim 2: Scale RCSB PDB operational capabilities to meet projected growth in structure data and complexity coming from studies of molecular machines using two rapidly evolving experimental methods (serial femtosecond X-ray crystallography or SFX and 3DEM) that have already eclipsed some of our service capabilities. Specific Aim 3: Implement deposition-validation-biocuration, archiving, and online delivery/tooling for structure data from emerging structure determination methods that combine 3DEM and/or macromolecular crystallography with multiple measurement techniques not currently supported in the PDB. These Integrative/Hybrid Methods structures are in the vanguard of cell/organismal biology research. Continued access to PDB data and RCSB PDB Services will drive publication of innovative research across the biomedical sciences, drug discovery and development, patent applications, and innovations leading to discovery and development of life-changing biopharmaceutical products and formation of new US companies.