Core 2: Animal Models

项目摘要-核心2 核心2（动物模型核心）的总体目标是支持动物模型的开发和使用 在这个计划项目补助金（PPG）中使用。动物模型核心的指导原则是有效的 动物的规划和利用，加工和诊断的标准化，服务的可靠性， 动物模型的一致性评价。核心内提供的基本专业知识是实验室动物 医学、临床和解剖病理学。该中心设在教育部，并由该部协调。 兽医生物科学Stefan Niewiesk博士，拟定的核心总监，一直担任动物核心总监 自2004年以来，他是PPG所有主要研究者的积极合作者。尼维斯克博士是一位 兽医微生物学和实验动物医学（欧洲学院）认证。Krista La Perle博士 （合作研究者），谁是由美国兽医病理学院认证将提供病理 核心评价。PPG的总体主题是分析HTLV-1感染如何诱导 CD 4 T细胞的增殖，病毒潜伏期和蛋白质如何影响和维持转化， 肿瘤细胞与它们的微环境相互作用。急性感染后，HTLV-1在生物体中持续存在， 最终导致CD 4 T细胞的寡克隆转化和生长。成立后， 病毒潜伏期的再激活（项目3：Ratner），肿瘤发生由病毒蛋白Hbz驱动（项目1： 绿色）。这些白血病细胞扩散到整个机体并引起骨质溶解（项目2：Weilbaecher）。 所有项目将使用成人T细胞白血病的兔和小鼠模型，这些模型已经建立和/或 核心2使用HTLV-1的分子克隆。

PROJECT SUMMARY – CORE 2 The overall goal of Core 2 (Animal Model Core) is to support the development and use of the animal models utilized within this Program Project Grant (PPG). The guiding principles for the animal model core are efficient planning and utilization of animals, standardization of processing and diagnoses, reliability of service and consistent evaluation of animal models. The fundamental expertise provided within the core is laboratory animal medicine and clinical and anatomic pathology. The Core is housed and coordinated in the Department of Veterinary Biosciences. Dr. Stefan Niewiesk, proposed Core Director has been Director of the Animal Core since 2004 and is an active collaborator with all of the principal investigators for the PPG. Dr. Niewiesk is a certified in Veterinary Microbiology and in Laboratory Animal Medicine (European College). Dr. Krista La Perle (Co-Investigator), who is certified by the American College of Veterinary Pathology will provide pathological evaluations for the Core. The overall theme of the PPG is to analyze how infection with HTLV-1 induces proliferation of CD4 T cells, how viral latency and proteins influence and maintain transformation and how the tumor cells interact with their microenvironment. After acute infection, HTLV-1 persists in the organism, eventually leading to oligoclonal transformation and outgrowth of CD4 T cells. After establishment and reactivation of viral latency (Project 3: Ratner), tumorigenesis is driven by the viral protein Hbz (Project 1: Green). These leukemic cells spread throughout the organism and cause osteolysis (Project 2: Weilbaecher). All projects will use rabbit and mouse models for adult T cell leukemia, which have been established and/or utilized by Core 2 using a molecular clone of HTLV-1.