LUMICKS C-TRAP

LUMICKS C-陷阱

• 批准号: 10414626
• 负责人: Christoph F Schmidt
• 金额: $ 60万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414626
• 关键词: Arts; Biology; Cell Adhesion; Cells; Cellular biology; Chemistry; Color; Confocal Microscopy; Custom; DNA; Dimensions; Engineering; Enzymes; Equipment; Family; Filopodia; Fluorescence; Funding; Housing; Image; Laboratories; Light; Microfluidics; Minor; Monitor; Motion; Motor; North Carolina; Physics; Reaction; Request for Proposals; Research; Resolution; Schools; Science; Signal Transduction; Specialist; System; Time; Training; Universities; Virion; design; experimental study; instrument; laser tweezer; light microscopy; mechanical signal; medical schools; medical specialties; optical traps; single molecule; spectroscopic imaging; transmission process

Project Summary In this proposal we request funds to purchase a LUMICKS C‐Trap® from LUMICKS USA Inc. This is a cutting‐edge instrument combining multi‐trap optical tweezers, multi‐color fluorescence confocal microscopy and multi‐channel microfluidics. The instrument provides extremely precise ways to manipulate single molecules or biomolecular aggregates, while monitoring forces and motions with sub‐pN and sub‐nm resolution, and, at the same time, imaging with single‐molecule resolution. The microfluidic systems allows the user to rapidly vary solution conditions during experiments. This instrument brings a new dimension to the study of biomolecular dynamics and cell biology, that of force, up to now limited to custom‐ designed specialty equipment in very few laboratories worldwide, built and operated by highly trained specialists. The C‐Trap® will support applications ranging from the study of DNA enzymes, cytoskeletal motors, subcellular force transmission, the fusion of virus particles to host cells, the function of mechanosensitive channels, the forces exerted and the signals received by cellular filopodia, cell adhesion, to cellular force fluctuations and reactions to mechanical signals. The C‐Trap® will be housed in the interdisciplinary Duke French Family Science Center, housing parts of the chemistry, biology and physics departments, within the Advanced Light Imaging & Spectroscopy (ALIS) laboratory, and will be managed and maintained by the Light Microscopy Central Facility (LMCF). It will be easily accessible to the initial major and minor user group of 14 PIs from Duke's Trinity School of Arts and Sciences, the Pratt Engineering School and the Duke University Medical School as well as from the School of Medicine of the University of North Carolina and the Department of Physics of North Carolina State University. It will be the only such instrument at Duke University and in the research triangle.

项目摘要 在此提案中，我们要求资金从Lumicks USA Inc.购买Lumicks C -Trap®。 这是一种结合多陷阱光学镊子的尖端仪器，多色 荧光共聚焦显微镜和多通道微流体。乐器提供的 操纵单分子或生物分子骨料的极为精确的方法，而 通过子PN和子NM分辨率监测力和运动，同时， 具有单分子分辨率的成像。微流体系统允许用户快速 在实验过程中变化的溶液条件。该乐器为 生物分子动力学和细胞生物学的研究，直到现在仅限于定制 在全球很少的实验室中设计的专业设备，由 训练有素的专家。 C -TRAP®将支持从研究 DNA酶，细胞骨架电动机，亚细胞力传播，病毒的融合 宿主细胞的颗粒，机械敏感通道的功能，施加的力和 细胞丝状，细胞粘附，细胞力波动和 对机械信号的反应。 C -Trap®将安置在跨学科的法国杜克法国家庭科学中心， 化学，生物学和物理部门的住房部分，在先进的光线下 成像与光谱法（ALIS）实验室，将由光线管理和维护 显微镜中央设施（LMCF）。最初的专业和小型专业很容易访问 Pratt Engineering Duke Trinity Arts＆Sciences的14个PI的用户组 学校和杜克大学医学院以及 北卡罗来纳大学和北卡罗来纳州物理系 大学。它将是杜克大学和研究的唯一这样的乐器 三角形。