LUMICKS C-TRAP

LUMICKS C-陷阱

• 批准号: 10414626
• 负责人: Christoph F Schmidt
• 金额: $ 60万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10414626
• 关键词: Arts; Biology; Cell Adhesion; Cells; Cellular biology; Chemistry; Color; Confocal Microscopy; Custom; DNA; Dimensions; Engineering; Enzymes; Equipment; Family; Filopodia; Fluorescence; Funding; Housing; Image; Laboratories; Light; Microfluidics; Minor; Monitor; Motion; Motor; North Carolina; Physics; Reaction; Request for Proposals; Research; Resolution; Schools; Science; Signal Transduction; Specialist; System; Time; Training; Universities; Virion; design; experimental study; instrument; laser tweezer; light microscopy; mechanical signal; medical schools; medical specialties; optical traps; single molecule; spectroscopic imaging; transmission process

Project Summary In this proposal we request funds to purchase a LUMICKS C‐Trap® from LUMICKS USA Inc. This is a cutting‐edge instrument combining multi‐trap optical tweezers, multi‐color fluorescence confocal microscopy and multi‐channel microfluidics. The instrument provides extremely precise ways to manipulate single molecules or biomolecular aggregates, while monitoring forces and motions with sub‐pN and sub‐nm resolution, and, at the same time, imaging with single‐molecule resolution. The microfluidic systems allows the user to rapidly vary solution conditions during experiments. This instrument brings a new dimension to the study of biomolecular dynamics and cell biology, that of force, up to now limited to custom‐ designed specialty equipment in very few laboratories worldwide, built and operated by highly trained specialists. The C‐Trap® will support applications ranging from the study of DNA enzymes, cytoskeletal motors, subcellular force transmission, the fusion of virus particles to host cells, the function of mechanosensitive channels, the forces exerted and the signals received by cellular filopodia, cell adhesion, to cellular force fluctuations and reactions to mechanical signals. The C‐Trap® will be housed in the interdisciplinary Duke French Family Science Center, housing parts of the chemistry, biology and physics departments, within the Advanced Light Imaging & Spectroscopy (ALIS) laboratory, and will be managed and maintained by the Light Microscopy Central Facility (LMCF). It will be easily accessible to the initial major and minor user group of 14 PIs from Duke's Trinity School of Arts and Sciences, the Pratt Engineering School and the Duke University Medical School as well as from the School of Medicine of the University of North Carolina and the Department of Physics of North Carolina State University. It will be the only such instrument at Duke University and in the research triangle.

项目摘要 在本提案中，我们申请资金从LUMICKS USA Inc.购买LUMICKS C‐Trap®。 这是一种结合了多阱光镊、多色 荧光共聚焦显微镜和多通道微流体。仪器提供 以极其精确的方式操纵单分子或生物分子聚集体， 以亚pN和亚nm分辨率监测力和运动，同时， 单分子分辨率成像微流体系统允许用户快速地 在实验过程中改变溶液条件。这一文书为《公约》提供了一个新的层面， 生物分子动力学和细胞生物学的研究，力的研究，到目前为止仅限于自定义 在世界各地的少数实验室设计专业设备，由 训练有素的专家。C‐Trap®将支持从研究 DNA酶，细胞骨架马达，亚细胞力传递，病毒融合 颗粒对宿主细胞的作用，机械敏感通道的功能，施加的力和 细胞丝状伪足、细胞粘附、细胞力波动和 对机械信号的反应。 C‐Trap®将被安置在跨学科的杜克法国家庭科学中心， 容纳部分化学，生物和物理部门，在先进的光 成像和光谱（ALIS）实验室，并将由光管理和维护 显微镜中心设施（LMCF）。它将很容易地访问到最初的主要和次要的 来自杜克Trinity艺术与科学学院、普拉特工程学院的14名PI的用户组 学校和杜克大学医学院以及从医学院的 北卡罗来纳州大学和北卡罗来纳州物理系 大学这将是杜克大学和研究中唯一的此类仪器 三角