Genomics, Bioinformatics, and Molecular Imaging Core

基因组学、生物信息学和分子成像核心

• 批准号: 10414532
• 负责人: Malwina Czarny-Ratajczak
• 金额: $ 54.49万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10414532
• 关键词: Aging; Applications Grants; Bioinformatics; Biological Assay; Biomedical Research; Biometry; Biostatistics Core; Cancer Center; Capillary Electrophoresis; Cell Line; Cells; Center for Translational Science Activities; Centers of Research Excellence; Chromium; Client; Clinical Sciences; Communities; Computational Biology; DNA; DNA sequencing; Data; Data Analyses; Data Analytics; Development; Dideoxy Chain Termination DNA Sequencing; Epidemiology; Equipment; Experimental Designs; Funding; Gene Expression; Genes; Genomics; Genotype; Goals; Growth; Human Resources; Louisiana; Luciferases; Measurement; Measures; Mentors; Microsatellite Repeats; Molecular; Names; Peer Review; Phase; Principal Investigator; Productivity; Proteomics; Publications; Quality Control; RNA; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Services; Source; System; Technology; Time; United States National Institutes of Health; Universities; Validation; XCL1 gene; base; bioinformatics pipeline; design; equipment acquisition; exome; increased appetite; instrumentation; laboratory equipment; miRNA expression profiling; molecular imaging; next generation sequencing; operation; transcriptome; transcriptome sequencing

The objective of the Genomics, Bioinformatics, and Molecular Imaging Core is to support and to enhance the research productivity of both COBRE and non-COBRE investigators. This is measured by our contributions to their peer-reviewed publications and grant applications. Our clients utilize our services from the study planning and design phase through the data analysis and presentation phase. The Core currently provides services for next-generation sequencing (NGS), DNA Sanger sequencing, genotyping, cell line validation, bioinformatics and biostatistics support, as well as other equipment and services. In Phase III, we will further enhance our core and organize its operations under three co-directors who have complementary expertise that encompasses all aspects of services offered. Because of these enhancements, we added ‘Molecular Imaging’ to our moniker. We will pursue the following specific aims: (1) Maintain access and services for next-generation sequencing (RNA- Seq, WES, miRNA-Seq, Ampli-Seq), Sanger DNA sequencing with capillary electrophoresis, real-time PCR applications, measurements for luciferase assays, DNA and RNA isolation, quantitation and quality control of DNA and RNA with the Agilent 2100 Bioanalyzer and cell line validation for investigators. (2) Expand the molecular portfolio of core services and implement new NGS applications by establishing Visium Spatial Gene Analysis using High-plex Proteomics and Whole Transcriptome Analysis, Chromium Single Cell Gene Expression and Multiome ATAC + Gene Expression using 10x Genomics and Illumina systems. (3) Continue biostatistics and bioinformatics support for data generated by the core and outside the core and develop new bioinformatics pipelines. (4) Expand and maintain sustainability and growth of the Core, by implementing recharge to all services and increase it over the course of Phase III and identify and deploy other sources of support. Our enhanced instrumentation and expanded personnel will make this possible, and this will help satiate the ever- increasing appetite for all of these applications. Our data analytic services will allow our clients to receive data that is not only clean and valid quantitatively but also has been put through a bioinformatics pipeline that makes it relevant biologically in broader terms. Both the biostatistics and bioinformatics components of the Core will contribute to the development of new and better approaches to the handling of the data our investigators generate.

基因组学、生物信息学和分子成像核心的目标是支持和加强 科布雷和非科布雷调查人员的研究效率。这是通过我们对以下方面的贡献来衡量的 他们的同行评议的出版物和赠款申请。我们的客户利用我们的学习规划服务 并通过数据分析和展示阶段进行设计。该核心目前提供以下服务 下一代测序(NGS)、DNA Sanger测序、基因分型、细胞系验证、生物信息学和 生物统计支助以及其他设备和服务。在第三阶段，我们将进一步提升我们的核心和 在三名联合董事的领导下组织运营，他们拥有互补的专业知识，涵盖所有 所提供服务的各个方面。由于这些改进，我们给我们的绰号加上了“分子成像”。我们 将追求以下具体目标：(1)保持对下一代测序(RNA- SEQ，WES，miRNA-Seq，Ampli-Seq)，毛细管电泳桑格DNA测序，实时荧光PCR 荧光素酶分析的应用、测定、DNA和RNA的分离、定量和质量控制 DNA和RNA与安捷伦2100生物分析仪和细胞系验证的研究人员。(2)拓展 核心服务的分子组合，并通过建立维西姆空间基因来实施新的NGS应用 铬单细胞基因高复合蛋白质组学和全转录组学分析 利用10x基因组学和Illumina系统进行表达和多组体ATAC+基因的表达。(3)继续 为核心和核心外产生的数据提供生物统计和生物信息学支持，并开发新的 生物信息学管道。(4)通过实施充值，扩大和保持核心的可持续性和增长 并在第三阶段增加，并确定和部署其他支助来源。我们的 改进的仪器设备和扩充的人员将使这一点成为可能，这将有助于满足日益- 对所有这些应用的兴趣与日俱增。我们的数据分析服务将允许我们的客户接收数据 这不仅在数量上是干净和有效的，而且已经通过了生物信息学管道，使 它在更广泛的生物学意义上是相关的。核心的生物统计和生物信息学部分都将 帮助开发新的更好的数据处理方法，我们的调查人员 生成。