Oral microbiome establishment and development of Latinx Children at the US-Mexico border

美墨边境拉丁裔儿童口腔微生物群的建立和发展

• 批准号: 10644580
• 负责人: Yan Wang
• 金额: $ 13.92万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10644580
• 关键词: 16S ribosomal RNA sequencing; Adult; Age; Algorithms; Allergic Disease; Antibiotics; Bacteria; Birth; Breast Feeding; Cardiovascular Diseases; Caregivers; Child; Child Health; Childhood; Chlorhexidine; Chronic; Collaborations; Complex; Control Groups; Counseling; County; Data; Dental caries; Dentistry; Development; Diet; Disease; Early Intervention; Ecosystem; Environment; Foundations; Freezing; Funding; Future; Goals; Health; Healthcare; High Prevalence; Hispanic; Hispanic Populations; Horizontal Disease Transmission; Human; Infant; Intervention; Knowledge; Latinx; Life; Literature; Los Angeles; Measures; Mentored Research Scientist Development Award; Mentors; Mentorship; Mexico; Microbe; Modeling; Modernization; Mothers; Mouth Diseases; Neonatal; Newborn Infant; Oral; Oral Characters; Oral cavity; Oral health; Oral mucous membrane structure; Outcome; Parents; Participant; Pattern; Pediatric Dentistry; Placenta; Placental microbiome; Play; Population; Postpartum Period; Pregnancy Complications; Preschool Child; Prevention program; Preventive; Proxy; Public Health; Qualifying; Regimen; Research; Research Activity; Research Personnel; Role; Sampling; Schedule; School Dentistry; Second Pregnancy Trimester; Statistical Data Interpretation; Surface; Surveys; Techniques; Therapeutic; Time; Tooth Diseases; Tooth structure; Training; United States; United States National Institutes of Health; Virus; Visit; Weight Gain; Work; Youth; autism spectrum disorder; biomarker discovery; biomarker identification; career; career development; deciduous tooth; disorder risk; early childhood; ethnic disparity; evidence base; experience; fungus; health disparity; health disparity populations; high risk; immune function; innovation; insight; kindergarten; medical schools; microbial; microbiome; microbiome composition; microbiome research; microbiota; microorganism; oral microbial community; oral microbiome; peer; personalized therapeutic; prenatal; prevent; racial disparity; randomized, clinical trials; research and development; risk prediction; saliva sample; transmission process; treatment group

PROJECT SUMMARY/ABSTRACT Early life interactions between the microbiome and its human host are responsible for an array of immune functions and future health outcomes. It is essential to study this period during early life when the oral microbiome changes from highly dynamic infant microbiota towards a more stable adult-like microbiota. The oral microbiome plays an essential role in oral and systemic health. As such, the study of the oral microbiome may serve as a harbinger for children’s health. Understanding the establishment and early development of the oral microbiome, and its association with the early childhood caries (ECC), may provide innovative solutions for personalized, predictive, and preventive pediatric dentistry. This Mentored Research Development Award K01 study seeks to understand the dynamic changes of the oral microbiome during the first three years of life among children who were born along the United States (US)-Mexico border. These children experience a high risk of dental caries and other oral diseases; therefore, providing a complex environment for the microbiome. The study aims are: (1) to understand the establishment of newborn oral microbiome among Latinx/Hispanic children living along the US-Mexico border by using salivary samples at prenatal (from mother), 4-, 9-, 12-, 24-, 36-months to characterize their oral microbiome establishment from birth to a full set of twenty primary teeth; (2) to compare children’s oral microbiome composition between control and treatment groups (mothers received therapeutic three-month regimen of chlorhexidine mouth rinse to reduce the bacteria that causes tooth decay when their children were between the ages of four to six months) while both groups received oral health counseling; and (3) to develop statistical expertise in modeling the changes of oral microbiota and their association with ECC controlling for confounding factors. This proposed research will use three recently developed Bayesian-based ML algorithms to model the temporal patterns of the oral microbiome and evaluate their ability to predict ECC. The K01 will support Dr. Yan Wang to develop expertise in: (1) oral microbiome development in early childhood, (2) statistical analysis for oral microbiome data, and (3) biomarker discoveries using saliva samples. To achieve these training goals and research activities, Dr. Wang has assembled a highly qualified mentorship team with substantial experience in mentoring early-career investigators and with expertise in oral microbiome research among children. Dr. Wang’s primary mentors at UCLA, Dr. Grace Aldrovandi (School of Medicine) and Dr. David Wong (School of Dentistry), are highly experienced NIH-funded investigators. Co-mentor Dr. Francisco Ramos-Gomez (School of Dentistry) is a leading expert in pediatric oral health, especially among Latinx/Hispanic children. The proposed research and training aims will build strong research capacities and collaborations for Dr. Yan Wang. In addition, this work will advance Dr. Yan Wang’s research career in analytical and translational oral microbiome research.

项目摘要/摘要 微生物群与其人类宿主之间的早期生命相互作用负责一系列免疫 功能和未来的健康结果。在早期生活中研究这一时期是必要的，当口腔 微生物组从高度动态的婴儿微生物区系向更稳定的成年型微生物区系转变。这个 口腔微生物群在口腔和全身健康中起着至关重要的作用。因此，对口腔微生物组的研究 可能是儿童健康的先兆。对中国传统文化的建立和早期发展的认识 口腔微生物组及其与幼儿龋齿(ECC)的关系可能为以下疾病提供创新的解决方案 个性化、预测性和预防性的儿科牙科。本年度导师研究发展奖K01 这项研究试图了解口腔微生物群在生命的前三年的动态变化 在美国-墨西哥边境出生的儿童中。这些孩子经历了一种兴奋 有患龋齿和其他口腔疾病的风险；因此，为微生物群提供了复杂的环境。 本研究的目的是：(1)了解拉美裔/拉美裔新生儿口腔微生物群的建立情况 居住在美墨边境的儿童在产前使用唾液样本(母亲)，4-，9-，12-，24-， 36个月来描述他们从出生到全套20颗乳牙的口腔微生物群建立； (2)比较对照组和治疗组(母亲)儿童口腔微生物组的组成 接受为期三个月的洗必泰含漱液治疗，以减少引起 当他们的孩子在四到六个月大的时候)，而两组人都接受了口服 健康咨询；以及(3)发展统计专业知识，模拟口腔微生物区系的变化及其 与ECC控制混杂因素的关联。这项拟议的研究将使用最近的三个 开发了基于贝叶斯的最大似然算法对口腔微生物组的时间模式进行建模和评估 他们预测ECC的能力。K01将支持王燕博士在以下方面发展专业知识：(1)口腔微生物组 儿童早期发展，(2)口腔微生物组数据的统计分析，以及(3)生物标记物的发现 使用唾液样本。为了实现这些培训目标和研究活动，王博士组织了一个 高素质的指导团队，在指导早期职业调查人员方面具有丰富的经验，并 儿童口腔微生物组研究方面的专业知识。王博士在加州大学洛杉矶分校的主要导师格雷斯博士 Aldrovandi(医学院)和David Wong(牙科学院)经验丰富，由NIH资助 调查人员。共同导师弗朗西斯科·拉莫斯-戈麦斯博士(牙科学校)是一位领先的儿科口腔专家 健康，特别是在拉丁裔/西班牙裔儿童中。拟议的研究和培训目标将建立强大的 王燕博士的研究能力和合作。此外，这项工作将推进王严博士的 分析和翻译口腔微生物组研究的研究生涯。