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Phosphoinositide signaling in autophagy

自噬中的磷酸肌醇信号传导

基本信息

批准号：
10652628
负责人：
Jie Chen
金额：
$ 31.06万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10652628
关键词：
Aging Animal Model Autophagocytosis Autophagosome Binding Biochemical Biogenesis Biological Assay Biophysics Cell Culture Techniques Cell membrane Cell physiology Cells Cellular biology Collaborations Complex Cyclic AMP-Dependent Protein Kinases Data Development Disease Ensure FRAP1 gene Family Grant Guanine Nucleotide Exchange Factors Human In Vitro Injury Kinetics Link Lipids Lysosomes Mammalian Cell Mediating Membrane Minor Modeling Molecular Muscle Mutation Analysis N-terminal Outcome PH Domain Pathway interactions Phosphatidylinositols Phosphorylation Phosphorylation Site Physiological Play Process Protein Analysis Protein Isoforms Protein Kinase C Proteins Regenerative capacity Regulation Rho-associated kinase Role Signal Transduction Site Skeletal Muscle Tissues Work detection assay human disease in vivo inhibitor insight late endosome molecular dynamics mouse model muscle regeneration mutant nervous system disorder new therapeutic target novel novel therapeutic intervention single molecule stoichiometry

项目摘要

PROJECT SUMMARY Phosphoinositides (PIPs) are minor components of the eukaryotic membrane but major regulators of cellular functions. The seven PIPs are critically involved in nearly every aspect of cell physiology. One of the cellular processes regulated by PIPs is autophagy, a process essential for a broad range of cellular functions and tissue development, and dysregulated in many human diseases. Found on late endosomes and lysosomes, PI(3,5)P2 is necessary for autophagosome maturation, and dysregulation of PI(3,5)P2 biogenesis has been linked to several neurological disorders through defective autophagy. However, the mechanism by which PI(3,5)P2 regulates autophagy is poorly understood. PIP signaling is often mediated by lipid-protein interactions. Our efforts in the last grant cycle have led to the development of a single-molecule assay that detects lipid interaction with proteins in mammalian whole-cell lysates, using which we have discovered widespread PIP interactions within the large family of human pleckstrin homology (PH) domain-containing proteins. XPLN, with dual activities as a RhoA guanine nucleotide exchange factor (GEF) and an endogenous inhibitor of mammalian target of rapamycin complex 2 (mTORC2), has emerged as a novel PI(3,5)P2-interacting protein, and we have also discovered that XPLN regulates autophagy in vivo. Guided by the working hypothesis that XPLN is an effector of PI(3,5)P2 and plays a central role in mediating PIP signaling in the regulation of autophagy, our proposed studies will decipher the biochemical basis of XPLN-PIP interactions and how they control XPLN activity and function. The role of XPLN phosphorylation by protein kinase C will also be investigated. We will ask how those biochemical mechanisms underlie the regulation of autophagy in mammalian cells. Finally, physiological relevance of the new mechanisms will be probed in a mouse model of injury-induced skeletal muscle regeneration, for which autophagy is required. Our expertise in lipid signaling, strong preliminary data, and a unique combination of biochemical, biophysical, cell biology, and animal model approaches will ensure a successful outcome that is likely to have significant impact on the biochemical and functional understanding of PIP signaling and regulation of autophagy.

项目总结肌醇磷脂(PIP)是真核膜的次要成分，但却是细胞的主要调节因子。功能。这七个PIP几乎涉及细胞生理学的方方面面。其中一种细胞 PIP调控的过程是自噬，这是一种对广泛的细胞功能和组织至关重要的过程发育，并在许多人类疾病中失调。在晚期内体和溶酶体上发现的PI(3，5)P2 是自噬体成熟所必需的，PI(3，5)P2生物发生的失调与通过有缺陷的自噬引起的几种神经系统疾病。然而，PI(3，5)P2 对自噬的调控知之甚少。PIP信号通常是通过脂-蛋白相互作用来调节的。我们的在上一个赠款周期中的努力导致了一种检测脂质相互作用的单分子分析的发展与哺乳动物全细胞裂解物中的蛋白质相互作用，我们发现了广泛的PIP相互作用在人类Pleckstrin同源(PH)结构域包含蛋白的大家族中。XPLN，具有双重活动作为一种RhoA鸟嘌呤核苷酸交换因子和哺乳动物靶标的内源性抑制物雷帕霉素复合体2(MTORC2)是一种新型的PI(3，5)P2相互作用蛋白。发现XPLN在体内调节自噬。以XPLN是效应器的工作假设为指导 PI(3，5)P2，并在调节自噬的PIP信号中发挥核心作用，我们建议研究将破译XPLN-PIP相互作用的生化基础，以及它们如何控制XPLN活性和功能。我们还将研究蛋白激酶C对XPLN磷酸化的作用。我们会问这些是如何生物化学机制是调节哺乳动物细胞自噬的基础。最后，生理学新机制的相关性将在损伤诱导的骨骼肌小鼠模型中进行探索再生，这需要自噬。我们在脂质信号方面的专业知识，强大的初步数据，以及生化、生物物理学、细胞生物学和动物模型方法的独特组合将确保成功的结果可能会对生化和功能的理解产生重大影响 PIP信号和自噬的调节。