Core C TEST IT

皮肤形成与环境的主要界面，其中角质形成细胞是先天免疫系统的关键组成部分。 免疫系统健康皮肤的扰动可通过皮肤免疫反应导致炎症。 因此，皮肤的免疫学是SBDRC的一个重要焦点，其具有“角质形成细胞及其 微环境”为主题。皮肤免疫系统需要一组相互作用的先天性和适应性免疫 可以在原位研究的细胞，在单细胞水平上进行研究的细胞，或重建以重建 皮肤的免疫状况。翻译和实验皮肤测试和免疫的目的 跟踪（测试它）的核心是支持和促进SBDRC科学家感兴趣的人类和 健康和疾病中的鼠皮肤免疫应答。核心会将各种免疫细胞从 健康和患病的皮肤，包括在单细胞水平，并提供表征的准备服务 这些细胞的直接或功能测定。使用Vectra多光谱成像设备，核心将协助 在设计实验，允许原位监测人类和小鼠的皮肤样品，外植体和工程 皮肤核心将为皮肤和血液中免疫细胞的有效扩增提供指导， 通过皮肤组织工程和形态学与3D重建的人皮肤或外植体重组， (STEM核心）。在重建之前，可以用来自基因工程的产物对细胞进行基因操作。 工程，转导和纳米技术（GET iN）核心。这些工程化的角质细胞免疫细胞 然后模型将可用于进一步的免疫评估。TEST IT的独特和高度新颖的方面 核心将是提供一个皮肤挑战设施，用于体内监测。这将通过一个 专门的临床单位和工作人员测试暴露于影响皮肤免疫的环境因子， 无论是以潜在有害的方式，例如紫外线/UV光，还是以有益的方式，例如局部 抑制免疫反应的药物。核心将监督组织采集计划（TAP）， 其获得存档样本以及新鲜皮肤、血液和其他样本（包括通过体内 皮肤挑战设施），用于具有安全记录的中央生物储存库;分发编码的去识别组织 供科学家在个别实验室或服务中心使用。核心还将保持一个健康的名册， 志愿者和愿意参加IRB批准的皮肤激发试验的皮肤病患者 实验这些转化研究支持机制调查，并提供概念验证数据。 核心还侧重于培训有关免疫皮肤病学的研究社区，提供以下方面的专业知识： 设计涉及皮肤免疫系统的小鼠和人体实验， 免疫皮肤病学、免疫治疗学和免疫监测。这是一个新颖的核心， 研究人员进行先进的和高度转化的免疫皮肤研究。学习能力受到干扰 以受控的方式研究人类皮肤将极大地促进转化研究。

The skin forms a primary interface with the environment, with the keratinocyte a critical component of the innate immune system. Perturbation of healthy skin can lead to inflammation through cutaneous immune responses. As such, the immunology of skin is an important focus of a SBDRC with “The Keratinocyte and its Microenvironment” as its theme. he skin immune system entails an interactive set of innate and adaptive immune cells that can be studied in situ, taken apart for studies at the single cell level, or reconstructed to recreate immune conditions of the skin. The objective of the Translational and Experimental Skin Testing and Immune Tracing (TEST IT) Core is to support and promote the research of SBDRC scientists interested in human and murine cutaneous immune responses in health and disease. The Core will isolate various immune cells from healthy and diseased skin, including at the single cell level, and offer preparative services for the characterization of these cells directly or in functional assays. Using Vectra multispectral imaging equipment, the Core will assist in designing experiments allowing in situ monitoring of human and mouse skin samples, explants and engineered skin. The Core will provide guidance for the efficient expansion of immune cells from skin and blood to be recombined with 3D reconstituted human skin or explants by the Skin Tissue Engineering and Morphology (STEM Core). Prior to reconstruction, cells can be genetically manipulated with products from the Gene Engineering, Transduction and Nanotechnology (GET iN) Core. These engineered keratinocyte-immune cell models will then be available for further immune assessment. A unique and highly novel aspect of the TEST IT core will be to provide a skin challenge facility for in vivo monitoring. This will be accomplished through a dedicated clinical unit and staff to test exposure to environmental agents that affect skin immunologically, whether in a potentially deleterious way, such as ultraviolet/UV light, or in a beneficial way, such as topical medications that suppress immune responses. The Core will oversee the Tissue Acquisition Program (TAP), which obtains archival samples, as well as fresh skin, blood, and other samples (including through the in vivo skin challenge facility) for a central biorepository with secure records; coded, deidentified tissues are distributed to scientists for use in individual laboratories or the service Cores. The Core will also maintain a roster of healthy volunteers and individuals with skin disease who are willing to participate in IRB-approved, skin challenge experiments. These translational studies support mechanistic investigations and provide proof-of-concept data. The Core also focuses on training the research community about immunodermatology, offering expertise in designing mouse and human experiments involving the skin immune system and generating course material in immunodermatology, immunotherapeutics, and immune monitoring. This is a novel core that focuses on enabling investigators to perform advanced and highly translational immune skin research. The ability to study perturbed human skin in a controlled manner will greatly facilitate translational research.