Elucidating the structural insights into the BMP receptor mutations in PAH

阐明 PAH 中 BMP 受体突变的结构见解

• 批准号: 10659947
• 负责人: Akiko Hata
• 金额: $ 80.52万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10659947
• 关键词: ACVR1 gene; ACVRL1 gene; Activin Receptor; BMP2 gene; Binding; Biological Assay; Biophysics; Blood Vessels; Bone Morphogenetic Protein Receptor Gene; C-terminal; Catalytic Domain; Cell Nucleus; Cells; Cessation of life; Complex; Cryoelectron Microscopy; Deuterium; Development; Disease; Distal; Family; Gene Mutation; Gene Structure; Genes; Genetic; Goals; Heritability; Heterozygote; Homeostasis; Hydrogen; In Vitro; Investigation; Knowledge; Length; Ligand Binding; Ligand Binding Domain; Ligands; Light; Link; Lung; Maintenance; Maps; Mass Spectrum Analysis; Measures; Membrane; Membrane Biology; Methods; Modeling; Molecular; Molecular Conformation; Morbidity - disease rate; Mutagenesis; Mutation; Pathogenesis; Pathogenicity; Pathologic; Patients; Phosphorylation; Phosphotransferases; Physiological; Progressive Disease; Pulmonary arterial remodeling; Pulmonary artery structure; Receptor Activation; Receptor Gene; Receptor Serine/Threonine Kinase; Receptor Signaling; Regulation; Research; Rodent; Roentgen Rays; Role; Signal Pathway; Signal Transduction; Site; Smad Proteins; Structural Models; Structure; Tail; Time; Transforming Growth Factor beta; Transmembrane Domain; Variant; Vascular Diseases; Vascular Endothelial Cell; Work; X-Ray Crystallography; activin receptor-like kinase 1; bone morphogenetic protein receptors; extracellular; innovation; insight; interdisciplinary approach; lung microvascular endothelial cells; member; mortality; mutant; novel; novel therapeutics; primary pulmonary hypertension; pulmonary arterial hypertension; pulmonary arterial pressure; receptor; reconstitution; right ventricular failure; stem; structural biology; success; targeted treatment

PROJECT SUMMARY/ABSTRACT Pulmonary Arterial Hypertension (PAH) is a progressive disease that leads to death in 3 years if untreated. PAH is characterized by remodeling and eventually occlusion of the pulmonary arteries, followed by high PA pressure and right heart failure. Heterozygous mutations in the bone morphogenetic protein receptor type 2 gene (BMPR2) are the leading genetic cause of PAH. In patients with BMPR2 mutations, PAH develops years earlier, and in a more severe form, than in patients with normal BMPR2. Notwithstanding the recent progress in identifying the molecular and cellular consequences of BMPR2 mutations, no targeted therapy for BMPR2 carriers exist, nor the dire need for novel therapies has been met. A well-supported model of BMP signaling starts with a ligand binding to a group of transmembrane serine/threonine receptor kinases comprised of two type 1 receptor (BMRPI) and two type 2 receptors (BMPR2). The heterotetrameric active BMP receptor complex phosphorylates Smad proteins that carry the signal downstream to the nucleus. Gaps in this model include lack of understanding why the receptors need to be organized in a heterotetramer configuration to be active, and how the receptor kinases are activated. Consequently, our understanding of prevalent PAH mutations that localize to the BMPR2 intracellular regions remains unsatisfactory and prohibitive from advancing PAH-targeted therapies. Our recent studies have led to a discovery that kinase domain of BMPR2 forms a heterodimer with a type 1 BMP receptor kinase. Formation of the heterodimer is not sufficient to activate the type 1 kinase but is essential for ligand-induced receptor signaling, suggesting its essential role in assembly of the active receptor tetramer. Importantly, several BMPR2 mutants linked to PAH map to the heterodimer interface and inhibit ligand-induced downstream Smad signaling, supporting the physiological significance of the heterodimer interface. The goal of this application is to elucidate the molecular underpinnings of BMP receptor kinase activation and elucidate how poorly understood BMPR2 mutations trigger PAH by: (i) dissecting the role of the type 1/type 1 kinase oligomerization in the catalytic activation of the BMP receptor complex, downstream signaling, and vascular homeostasis, and (ii) gaining the structural understanding of the active type 1/type 2 kinase domain complexes alone and in the context of full- length BMP receptor tetramers. Upon completion, this study will define the significance of the non-catalytic interfaces present on BMP receptor kinases and provide insights into how BMPR2 mutations perturb the type 1/type 2 kinase interactions resulting in PAH. This knowledge will provide platform for the development of innovative novel PAH therapies.

项目总结/文摘