Microbiome Research Core

项目摘要-微生物组核心 微生物群落和分离物的表征是实现科学的生物学特性的关键因素。 拟议的代谢转化研究中心的目标。微生物组核心将 提供专门用于微生物组研究和培训的独特服务。核心的目标是支持一个 用于表征微生物群落和分离物的综合管道， 将产生可验证的假说来解释宿主-微生物组共生。我们将通过以下方式实现这一目标： 实现以下具体目标（SA）。SA 1.提供和维护最先进的技术， 用于表征微生物群落和分离物的高通量管道的程序。SA 2.提供 微生物组研究的专业知识，从实验设计和样本收集到数据分析， 生物信息学SA 3.通过专家咨询支持和发展微生物组研究和传播， 并与COBRE调查人员和公众开展外联活动。这件事的新奇之处在于 拟议的核心是联合收割机多个模块，研究人员可以插入任何点沿着管道或 访问整个管道以表征分离物或微生物群落。微生物组核心是一个 第一阶段中心支持的研究项目的重要组成部分，重点是基础研究 微生物与宿主的共生关系核心提供所需的专业知识、设备和软件 用于有机体、分子和生物信息学分析。核心维护的关键设备包括 微生物生物反应器，允许用户设置类似于宿主肠道的实验条件，并干扰 识别关键微生物过程的系统。Illumina MiSeq和Nanopore MinION将提供 测序能力来分析分子基础。与INBRE生物信息学合作 Microbiome Core将提供微生物组研究所需的生物信息学算法的专业知识。

Project Summary - Microbiome Core Characterization of microbial communities and isolates is a key element necessary to achieve the scientific objectives of the proposed Center in Transformative Research in Metabolism. The Microbiome Core will provide a unique service dedicated to microbiome research and training. The goal of the Core is to support a comprehensive pipeline for characterization of microbial communities and isolates, which when characterized will generate testable hypotheses explaining host-microbiome symbiosis. We will achieve this goal by accomplishing the following Specific Aims (SA). SA1. Provide and maintain state-of-the-art technologies and procedures for a high throughput pipeline that characterizes microbial communities and isolates. SA2. Provide expertise in microbiome research, from experimental design and sample collection to data analysis and bioinformatics. SA3. Support and grow microbiome research and dissemination through expert consultation, and by engaging in outreach events with COBRE investigators and the general public. The novelty of this proposed core is to combine multiple modules that researchers can plug into at any point along the pipeline or access the full pipeline to characterize either isolates or microbial communities. The Microbiome Core is an essential component of the Research Projects supported by the Phase 1 center that focus on basic research into microbe-host symbiosis in metabolism. The Core provides the expertise, equipment, and software required for organismal, molecular, and bioinformatic analysis. Key equipment maintained by the Core includes a microbial bioreactor that allows users to set up experimental conditions similar to host guts and perturb the system to identify key microbial processes. An Illumina MiSeq and Nanopore MinION will provide the sequencing power to analyze the molecular underpinnings. In collaboration with the INBRE Bioinformatics Core, the Microbiome Core will provide expertise on bioinformatic algorithms needed for microbiome research.