Discovery Science Collaborative for CKDu

项目摘要/摘要 CKDU肾科芯发现科学协作组的总体目标是带来最好的 对CKDU问题的调查方法和科学技术。我们将与菲尔德密切合作 流行病学网站、科学数据协调中心、NIDDK领导层、外部监督 委员会，和HHEAR设计临床表型研究和进行发现科学实验。 然后，我们将结合这些数据来阐明发生在不同类型的CKDU的病因和进展因素 农业社区。肾科学核心是一个多机构合作组织，大约有六个 核心课程：基因组学、病理学、转录学、生物标志物、生理学和生物信息学。这个 RSC的具体目标是：(1)确定患者的临床表型和自然病史 在CKDu流行地区的CKDu和未受影响的个人，比较CKDu站点和 确定不同地点和地区的异同程度；(2)引入尖端技术 用分子技术比较研究地点之间和研究地点内的CKDU的危险因素和病理生理学 理解疾病病因的共同和独特方面；(3)定义 Aki和CKDu在流行地区；以及(4)建立灵活、适应性强的基础设施，用于假设和 非基于假设的方法来理解病因学，这将形成正在进行的CKDU研究的核心。 RSC的领导团队在CKDu方面拥有丰富的工作经验，这是一段合作的历史 国际工作组，以及在其他NIDDK主要财团中的领导作用。这个团体聚集在一起 在肾病学、基因组学、肾脏病理学、数字病理学机器学习算法方面的专业知识，单一- 细胞转录学、生物标记物开发、蛋白质组学、代谢组学、肾脏生理学、生物信息学和 数据集成。我们还聘请了几位在新病原体发现、新小说方面有专长的顾问 成像技术和环境采样。这些研究将使我们更好地理解 肌萎缩侧索硬化症的基本病理生理学。

PROJECT SUMMARY / ABSTRACT The overall goal of the Discovery Science Collaborative for CKDu Renal Science Core is to bring the best investigative methods and scientific technology to the problem of CKDu. We will work closely with Field Epidemiology Sites, the Scientific Data Coordinating Center, NIDDK Leadership, the External Oversight Committee, and HHEAR to design clinical phenotyping studies and perform discovery science experiments. We will then integrate this data to elucidate the etiology and progression factors of CKDu occurring in diverse agricultural communities. The Renal Science Core is a multi-institutional collaborative organized around six Core components: Genomics, Pathology, Transcriptomics, Biomarkers, Physiology, and Bioinformatics. The specific aims of the RSC are to: (1) characterize the clinical phenotypes and natural history of patients with CKDu and unaffected individuals in CKDu-endemic regions, comparing features across CKDu sites and determining the degree of similarities and differences across sites and regions; (2) introduce cutting-edge molecular technologies to compare the risk factors and pathophysiology of CKDu across and within study sites to understand both shared and unique aspects of the etiology of disease; (3) define the relationship between AKI and CKDu in endemic regions; and (4) build a flexible, adaptable infrastructure for both hypothesis- and non-hypothesis-based approaches to understanding etiology that will form the core of ongoing CKDu studies. The leadership team of the RSC has extensive experience working on CKDu, a history of collaboration on international working groups, and leadership roles on other major NIDDK consortia. This group brings together expertise in nephrology, genomics, nephropathology, machine learning algorithms for digital pathology, single- cell transcriptomics, biomarker development, proteomics, metabolomics, renal physiology, bioinformatics, and data integration. We have also enlisted several advisors with expertise in novel pathogen discovery, novel imaging technologies, and environmental sampling. These studies will result in a greater understanding of the underlying pathophysiology of CKDu.