Comparative systems biology of apicomplexan cell division
Comparative systems biology of apicomplexan cell division
批准号:
10669790
负责人:
Manoj T Duraisingh
金额:
$132.72万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-07-21 至 2027-06-30
关键词:
ApicalApicomplexaAtlasesBabesiaBabesiosisBiologicalBiologyCell CycleCell Cycle ProgressionCell divisionCellsCellular AssayChemicalsChromatinChromosome SegregationCommunitiesComputer softwareCyclin-Dependent KinasesCyclinsCytoskeletonDNA biosynthesisDataData SetFamilyGene ExpressionGene Expression ProfileGenesGeneticGenetic TranscriptionHealthHeartHomologous GeneHumanInvadedLifeLife StyleMalariaMammalian CellMapsMediatingModelingMothersOpportunistic InfectionsOrganellesParasitesPathogenesisPathway AnalysisPharmaceutical PreparationsPlasmodiumPlasmodium falciparumResearchResolutionResourcesSarcocystisSurveysSystemSystems BiologyTimeToxoplasma gondiiToxoplasmosisTransposaseVariantVisualizationWorkasexualcausal variantcomparativeepigenomicsexperimental studyflexibilitygene networkgene regulatory networkinhibitorinnovationinterestknock-downnetwork architecturenovelnuclear divisionphenomenological modelsprogramsquery toolssingle cell sequencingsingle-cell RNA sequencingsoftware developmenttick transmissiontranscription factortranscriptometranscriptomicsweb appweb site
中文摘要
总结
顶复门寄生虫对人类健康有重大影响,例如恶性疟原虫引起疟疾
而弓形虫和巴贝虫属。引起机会性感染。虽然这个关系密切的团体
尽管它们有着专性的细胞内生活方式,但它们表现出多种无性细胞分裂模式。这些
寄生虫之间以及单一寄生虫物种内不同生命阶段之间的差异是不同的,但起始
终点始终是具有宿主细胞侵袭能力的“孢子”。每轮产生的孢子数
变化很大(从 2 到 90,000),并且可以通过重新洗牌功能模块以几种不同的方式展开
1) 母细胞骨架拆卸,2) DNA 合成和染色体分离 (D&S),3) 核分裂,
4) 孢子组装(出芽)。 Apicomplexa 不同的细胞分裂模式源于
模块的顺序和顺序以及模块重复的次数。在当前模型中,细胞
分裂在转录波介导的转录因子中进行,这些转录因子作用于靶基因,进而作用于靶基因
捆绑到功能模块中。然而,人们对它的组成、调节器和接线知之甚少。
不同的模块,以及这如何导致 Apicomplexa 细胞分裂模式的多样性。研究团队
假设这些问题可以通过比较系统生物学方法来回答,首先
代表不同的多样化和“外来”分裂细胞分裂模式的寄生虫,其中特定模块是
放大或以不同方式组合:分歧巴贝斯虫二元裂变、恶性疟原虫分裂生殖、肉孢子虫
无核分裂的神经元内多发生、弓形虫无性内生和弓形虫前性
终宿主中具有核分裂的内多性。这种方法利用了单细胞
测序革命与计算网络分析方法相结合。首先,单细胞
将生成并分析五种细胞分裂模式的转录组和表观基因组图谱,以定义
每个特定模块中包含的效应器。未表征的效应子的子集
核分裂和细胞骨架拆卸模块将通过基因敲除进行实验验证。
其次,化学和遗传扰动与单细胞测序相结合将能够组装
跨所有分裂模式的因果基因调控网络(GRN)。这些 GRN 中的候选模块控制器
将通过重新编程和/或遗传扰动实验来验证:改变(部分)分裂
特定寄生虫中的模式。这项工作将回答有关 apicomplexan 特定生物学的难以捉摸的问题
在几乎没有研究过的功能模块中,以及apicomplexan细胞分裂灵活性是如何连接的。第三,
拟议的工作将产生广泛的社区资源,包括单一表达和染色质
涵盖五种不同细胞分裂模式和寄生虫物种的可访问性图集。此外,数据集将
可通过基于网络的应用程序跨系统实时搜索任何感兴趣的生物特征
合并到 VEuPathDB 中,并能够查询数据以了解细胞分裂之外的生物学问题。
英文摘要
Summary
Apicomplexan parasites have major impacts on human health e.g. Plasmodium falciparum causes malaria
whereas Toxoplasma gondii and Babesia spp. cause opportunistic infections. Although this close-knit group
shares their obligate intracellular life styles, they display a wide variety of asexual cell division modes. These
differ between parasites as well as between different life stages within a single parasite species, but the start-
and end-point is always a host cell invasion competent ‘zoite’. The number of zoites made per division round
varies dramatically (from 2-90,000) and can unfold in several different ways by reshuffling the functional modules
of 1) mother cytoskeleton disassembly, 2) DNA synthesis and chromosome segregation (D&S), 3) karyokinesis,
and 4) zoite assembly (budding). Distinct cell division modes across Apicomplexa arise from variations in the
order and sequence of the modules as well as the number of module repetitions. In the current model, cell
division progresses in transcriptional waves mediated transcription factors that act on target genes that in turn
bundle into the functional modules. However, little is known of the composition, regulators and wiring of the
different modules, and how this leads to the diversity of cell division modes in Apicomplexa. The research team
hypothesizes that these questions can be answered by a comparative systems biology approach, starting with
parasites representing different diverse and ‘exotic’ division cell division modes wherein particular modules are
amplified, or combined differently: Babesia divergens binary fission, P. falciparum schizogony, Sarcocystis
neurona endopolygeny without karyokinesis, T. gondii asexual endodyogeny and T. gondii pre-sexual
endopolygeny with karyokinesis in the definitive host. This approach takes advantage of the single cell
sequencing revolution combined with computational network analysis approaches. Firstly, single cell
transcriptomic and epigenomic maps of the five cell division modes will be generated and analyzed to define the
effectors contained in each specific module. A subset of uncharacterized effectors in the poorly characterized
karyokinesis and cytoskeleton disassembly modules will be experimentally validated by gene knock-downs.
Secondly, chemical and genetic perturbations combined with single cell sequencing will enable the assembly of
causal gene regulatory networks (GRNs) across all division modes. Candidate module controllers in these GRNs
will be validated by reprogramming and/or genetic perturbation experiments: changing (parts of) the division
mode in specific parasites. This work will answer elusive questions regarding apicomplexan specific biology
within barely studied functional modules, as well as how apicomplexan cell division flexibility is wired. Thirdly,
the proposed work will produce extensive community resources comprising single expression and chromatin
accessibility atlases across five different cell division modes and parasite species. Moreover, data sets will be
searchable across systems in real time for any biological feature of interest by web-based Apps that will be
incorporated in VEuPathDB and enable querying the data for biological questions beyond cell division.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1371/journal.pbio.3001997
发表时间:
2023-01
期刊:
PLOS BIOLOGY
影响因子:
9.8
作者:
[Duraisingh, Manoj T., Gubbels, Marc-Jan, Zarringhalam, Kourosh]
通讯作者:
Zarringhalam, Kourosh
Linking metabolite sensing and gene expression in malaria parasites
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依托单位:
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依托单位:
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依托单位:
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资助金额:$39.88万
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依托单位:
Elucidating ligand-receptor interactions required for Plasmodium vivax blood-stage infection
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批准号:10443628
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资助金额:$39.88万
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项目类别:
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资助金额:$19.87万
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依托单位:
Genetic screens for erythrocyte determinants of protein trafficking in malaria parasites
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资助金额:$23.81万
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Functional analysis of erythrocyte determinants of malaria infection
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依托单位:
海外基金