Methods for Isotope Labelling of Glycans
聚糖的同位素标记方法
基本信息
- 批准号:10684319
- 负责人:
- 金额:$ 19.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAldehydesAlkylationAminationAminesAreaBenchmarkingBindingBiochemicalCarbohydratesCarbonCarbon MonoxideChemicalsChemistryCollectionComplementComplexCytolysisDataDerivation procedureDiagnosisDisaccharidesDiseaseDrug DesignEnzymatic BiochemistryEquilibriumGlycoconjugatesGlycoproteinsHeparan Sulfate ProteoglycanHeparin LyaseHeparitin SulfateHumanHuman MilkInterruptionIsotope LabelingIsotopesKineticsKnowledgeLabelLigandsLinkMass Spectrum AnalysisMediatingMedicineMetabolicMethodologyMethodsModernizationMonosaccharidesOligosaccharidesPathologic ProcessesPhenotypePhysiological ProcessesPlayPolysaccharidesPositioning AttributePreparationProcessProductionPropertyRadiolabeledReactionRoleSamplingSialic AcidsSourceStructureSulfateSystemTechniquesTechnologyTherapeuticTransition Elementsanaloganomerbiological systemscarbohydrate structurecarbonyl groupcatalystcostfeedinghydroxyl groupimaging studyin vivoinventionmetabolic engineeringnew technologynovelstable isotopesugartechnology developmenttimelinetool
项目摘要
PROJECT SUMMARY. Glycomics studies are crucial for correlating changes in glycan structure and abundance
to the phenotype of a biological system. Among the available platforms to facilitate glycomics analyses, labelling
with stable isotopes has been implemented to decipher structural and quantitative aspects of carbohydrates in
healthy and diseased states. Isotopically labelled saccharides are also a useful tool for in vivo metabolic profiling
and in mechanistic studies in chemistry and enzymology. While some structures are commercially available, a
generally applicable and non-destructive method for introducing stable heavy isotopes either in simple
monosaccharides or complex oligosaccharides are not available. To overcome these limitations, we will develop
a chemical technology based on carbon-13 labelling. To achieve this objective, we will pursue two specific aims:
In Aim 1, we will optimize catalytic methods for selective labelling of monosaccharides with carbon-13. This
method will selectively exchange the anomeric position with a heavy isotope from readily available sources of
carbon-13. In Aim 2, we will apply isotope labelling method to three classes of saccharides of biomedical
relevance, namely heparan sulfate proteoglycans, N-linked glycoproteins, and human milk oligosaccharides. Per
requirements of PAR-19-254, we will benchmark the new technologies against the current state-of-the-art
methods for oligosaccharide quantification. Upon completion of this project, a new catalytic technology for
labelling of carbohydrates and glycoconjugates will be available and will complement the current tools for
quantitative glycomics. In a broader sense, integration of these novel chemistries with modern mass
spectrometry techniques will provide better tools for therapeutic diagnosis.
项目总结。糖组学研究对糖结构和丰度变化的相关性至关重要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Maciej Walczak其他文献
Maciej Walczak的其他文献
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{{ truncateString('Maciej Walczak', 18)}}的其他基金
Dissecting the role of tau glycosylation in Alzheimer's disease
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10373087 - 财政年份:2021
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Synthesis and Chemical Biology of Thiopeptide Antibiotics
硫肽类抗生素的合成及化学生物学
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10211882 - 财政年份:2021
- 资助金额:
$ 19.16万 - 项目类别:
Synthesis and Chemical Biology of Thiopeptide Antibiotics
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9391514 - 财政年份:2017
- 资助金额:
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