权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Quantitative proteomic analysis of the aging brain after intracerebral hemorrhage

脑出血后衰老大脑的定量蛋白质组学分析

基本信息

批准号：
10708753
负责人：
Sangeetha Sukumari-Ramesh
金额：
$ 7.7万
依托单位：
AUGUSTA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-30 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10708753
关键词：
Acute Address Age Aging Animal Model Animals Antibodies Anticoagulants Bioinformatics Brain Brain Injuries Cell Separation Cells Cerebral hemisphere hemorrhage Chronic Phase Complex Data Development Disease Elderly Flow Cytometry Functional disorder Genetic Goals Grant Hospitals Human In Vitro Incidence Infiltration Inflammation Mediators Inflammatory Innate Immune System Investigation Ipsilateral Knowledge Label Laboratories Liquid Chromatography Macrophage Mass Spectrum Analysis Mediating Microglia Mission Molecular Mus National Institute of Neurological Disorders and Stroke Neurological outcome Pathology Pathway interactions Patient-Focused Outcomes Patients Phagocytes Phagocytosis Play Pre-Clinical Model Prevalence Process Prognosis Proteins Proteome Proteomics Public Health Reporting Research Research Personnel Role Stroke Therapeutic Intervention Time Translations adverse outcome aged aging brain brain repair cell type clinically relevant disability effective therapy experience improved in vivo molecular targeted therapies monocyte mortality novel novel therapeutic intervention patient population pharmacologic pre-clinical preclinical study response tandem mass spectrometry tool

项目摘要

Intracerebral hemorrhage (ICH) is a devastating subtype of stroke with no effective treatment. Lack of treatment options partly contributes to the poorly defined pathophysiology of ICH. Along these lines, despite the increasing prevalence of ICH in the elderly, the molecular level changes that occur in the aging brain after ICH remains largely enigmatic. To address this critical knowledge gap, two specific aims are proposed. Aim 1: To determine the age-induced changes in the brain proteome after ICH. Aim 2: To determine the age-induced protein level changes in microglia and brain-infiltrating monocyte-derived macrophages (BMDM) after ICH. The proposed studies employ a preclinical model of ICH and include unbiased and rigorous proteomics approaches such as label-free and TMT-labeling Mass spectrometry and bioinformatics approaches. Successful completion of the project would identify novel molecular regulators to improve the neurological outcomes after ICH in the elderly. Also, the studies would define, for the first time, the proteomic profile of acutely isolated microglia and macrophages, the cells that play key roles in brain damage and repair after ICH.

脑出血（ICH）是一种毁灭性的脑卒中亚型，没有有效的治疗方法。缺乏