Role of the FAAH-OUT locus in cutaneous wound healing
Role of the FAAH-OUT locus in cutaneous wound healing
批准号:
10707885
负责人:
Bryan Sun
金额:
$50.66万
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-09-21 至 2027-06-30
关键词:
AccelerationAcuteAldehydesAllelesBindingBiological AssayBiological ProcessCellsChromatinCicatrixClinicalDNADermatologyEnhancersEpitheliumFatty AcidsFibroblastsFoundationsGenesGeneticGenetic TranscriptionGenetic studyGenome engineeringGenotypeGoalsGrantHumanHydrolaseIn Situ HybridizationIndividualInjuryKnockout MiceLaboratoriesLearningLinkLipidsMedicalMessenger RNAMicroRNAsModelingMolecularMolecular ConformationMutagenesisNamesOrganoidsOutcomePathway interactionsPatientsPhenotypePhysiciansProcessPropertyProteinsRegulatory ElementResearchResearch PersonnelRoleSkinSkin TissueSkin injurySkin repairSkin wound healingStudy SubjectTestingTissuesUnited StatesUntranslated RNAWomanWound healing therapyaging populationchronic woundexperienceexperimental studygain of functiongenome sequencinggenomic locusimprovedinnovationinsightkeratinocyteloss of functionmigrationnew therapeutic targetnovelnovel therapeuticsolder womenrepair modelrestraintskin regenerationskin woundskin xenografttargeted treatmenttissue repairtranscriptomicswoundwound healingwound treatment
中文摘要
研究总结
英文摘要
RESEARCH SUMMARY
The proposal will study the unusual phenotype of a 69-year-old woman who displays exceptionally
accelerated skin wound healing with minimal scarring. Genome sequencing revealed that her
phenotype is caused by an ~8 kb deletion downstream of the fatty acid aldehyde hydrolase
(FAAH) gene that results in the loss of a long noncoding RNA (lncRNA) gene named FAAH OUT.
The goal of this proposal is to understand how the FAAH OUT deletion results in accelerated
wound healing. In the United States, the annual medical burden of chronic wounds exceeds $25
billion and continues to rise with the aging population. There is a critical need to improve
understanding of the biological processes underlying skin wound healing and to innovate new
therapies. In the first aim, we will study normal and wounded skin tissue from the study subject
using spatial transcriptomics, in situ hybridization, and xenograft skin repair models to understand
how the FAAH OUT locus regulates wound healing. In the second aim, we will elucidate the FAAH
OUT genetic interactome to gain insight to its molecular mechanisms. By studying a rare genetic
allele of an individual with markedly enhanced wound healing, this proposal aims to generate
novel insight to the role of lncRNAs in wound repair and discover new human-validated targets to
treat cutaneous wounds.
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Role of the FAAH-OUT locus in cutaneous wound healing
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批准号:10334272
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项目类别:
-
资助金额:$50.66万
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财政年份:2022
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负责人:Bryan Sun
-
依托单位:
Insights to Noncoding Disease Variants using Mosaic Diseases
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批准号:10244405
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项目类别:
-
资助金额:$36.95万
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财政年份:2021
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负责人:Bryan Sun
-
依托单位:
CRISPR Screen to Identify Novel Secreted Epidermal Regulators
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批准号:9807172
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项目类别:
-
资助金额:$6.63万
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财政年份:2019
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负责人:Bryan Sun
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依托单位:
Secreted Regulators of Epidermal Homeostasis
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批准号:9313177
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项目类别:
-
资助金额:$18.13万
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财政年份:2016
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负责人:Bryan Sun
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依托单位:
Role of Noncoding RNAs in Epidermal Differentiation
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批准号:8565649
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项目类别:
-
资助金额:$5.89万
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财政年份:2012
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负责人:Bryan Sun
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依托单位:
Role of Noncoding RNAs in Epidermal Differentiation
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批准号:8390210
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项目类别:
-
资助金额:$5.57万
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财政年份:2012
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负责人:Bryan Sun
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依托单位:
海外基金