ANIMAL CORE

在戒毒期之后再次吸毒是长期的重大障碍。 可卡因依赖的治疗。以前与药物使用有关的环境刺激或背景 滥用药物的启动剂量可能会引发强迫性寻求和吸毒的复发。 行为。动物核心B将为每个项目提供所有的实验对象 神经生物学成瘾研究中心(NARC)，涉及使用自我管理和 大鼠复发模型。大鼠将被插管，并接受自我静脉注射的训练。可卡因 使用药物强化的标准时间表。经过一段稳定的自我管理时期后，杠杆按下 因为毒品将会熄灭到标准，或者动物可以经历禁欲。然后受试者将被指导 用于脑组织提取、电生理学或行为评估的个别NARC项目 根据每个NARC的具体目标，对系统或现场指导的操作做出响应 项目。动物核心B的目标可以概括为： 1.为NARC中所有一致并满足特定标准的项目提供实验对象 关于药物自我管理的经验以及戒断、灭绝和/或 禁欲。动物核心将确保受试者将按照所有 经批准的动物使用程序，并与兽医人员一起饲养适当的动物 畜牧业。 2.有效管理动物对不同项目的分配，以确保统计上的相关性 及时向每个项目提供组大小和组复制。 3.保持一支训练有素的技术人员队伍，以有效地使用平衡的 小组设计，以及向NARC项目提供技术援助，这些项目将涉及进一步 寻毒行为的行为学评价。 4.为希望接受自我管理培训的初级调查人员提供NARC培训中心， 颈静脉插管、手术调理方法等相关实验操作。 NARC动物核心将作为所有NARC项目的共同基础。结果来自于 通过动物核心的综合研究将有望导致有用的药物治疗药物 可卡因滥用和依赖的治疗。

Relapse to drug use following periods of abstinence is a significant impediment in the long-term treatment of cocaine dependence. Environmental stimuli or contexts previously associated with drug use or priming doses of the abused drug can initiate relapse to compulsive drug-seeking and drug-taking behaviors. The Animal Core B will provide all of the experimental subjects for every project in the Neurobiology of Addiction Research Center (NARC) that involves the use of the self-administration and relapse model in rats. Rats will be catheterized and trained to self-administer intravenous (i.v.) cocaine using standard schedules of drug reinforcement. After a stable period of self-administration, lever pressing for drug will be extinguished to criterion, or animals may undergo abstinence. Subjects will then be directed towards individual NARC projects for brain tissue extraction, electrophysiology, or assessment of behavioral responding to systemic or site directed manipulations in accordance with the specific aims of each NARC project. The aims of Animal Core B can be summarized as: 1. Provide experimental subjects to all projects in the NARC that are consistent and satisfy specific criteria with regards to experience in drug self-administration and in the degree of withdrawal, extinction, and/or abstinence. The Animal Core will insure that subjects will be handled and treated according to all approved animal use procedures and, in conjunction with vivarium staff, maintain appropriate animal husbandry. 2. Efficiently manage the distribution of animals to the different projects to insure that statistically relevant group sizes and group replications are made available to each project in a timely manner. 3. Maintain a staff of well-trained technicians to efficiently generate experimental subjects using a balanced group design, as well as provide technical assistance to NARC Projects that will involve further behavioral evaluation of drug-seeking behavior. 4. Serve as a NARC training center for junior investigators who desire training in self-administration, jugular catheterization, operant conditioning methods, and other related experimental procedures. The NARC Animal Core will serve as the common foundation for all NARC projects. The results from integrated studies through the Animal Core will hopefully lead to useful pharmacotherapeutic agents for the treatment of cocaine abuse and dependence.