New Classes of Electron Paramagnetic Resonance Imaging Probes With High-Spin Metal Complexes

项目摘要/摘要 Zadrozny实验室的重点是设计用于非侵入性传感的金属络合物 生理化学的。这项努力的更广泛的目标是使分子探测器 克服电子顺磁共振成像(EPRI)的内在挑战 电子模拟常规1H磁共振成像。EPRI可以感知当地的化学物质，并可以产生 如果与1H MRI合并，则提供全面的身体化学和解剖图。现代EPRI 分子成像探针是需要危险的高能微波的有机自由基 用于核磁共振扫描仪的大磁场。因此，这两种技术仍然存在 已断开连接。对于EPRI通过与MRI集成实现生理化学成像，新的 必须开发探头，以避免在强磁场中产生高频微波。下一个 扎德罗兹尼实验室五年的工作包括探索高自旋金属络合物作为 EPRI中取代自由基的分子探针平台。金属的一个关键的固有优势 离子的特点是大零场分裂的独特的电子特征使得安全的可能性， 低频微波在强磁场下使用。因此，具有这一特征的金属络合物 可以提供一套全新的EPRI分子成像探针 有机自由基无法比拟的。然而，金属的所有基本EPR光谱性质 带有低频微波的复合体是未映射的。扎德罗兹尼实验室将对此进行修正 知识鸿沟。这项工作将使用合成无机化学和光谱分析来 (1)了解如何确定共振的频率/场以匹配 使用低频微波的核磁共振扫描仪(2)了解如何控制 低频EPR共振以提高分辨率，以及(3)如何合并自由基/金属 杂化分子中的化学，以获得金属和自由基的优点 分子探针系统。满足这些目标将提供一种新的成像探头 能够在常规核磁共振扫描仪中绘制生理化学图。

Project Summary/Abstract The focus of the Zadrozny laboratory is the design of metal complexes for noninvasive sensing of physiological chemistry. The broader goal of the effort is to make molecular probes that overcome inherent challenges in electron paramagnetic resonance imaging (EPRI), the unpaired electron analog to conventional 1H MRI. EPRI can sense local chemistry and could produce comprehensive chemical and anatomical maps of the body if merged with 1H MRI. Modern EPRI molecular imaging probes are organic radicals which require dangerous high-energy microwaves for use in the large magnetic field of an MRI scanner. Hence, the two techniques remain disconnected. For EPRI to enable imaging of physiological chemistry by integration with MRI, new probes must be developed to avoid high frequency microwaves at high magnetic fields. The next five years of the Zadrozny lab’s work involve exploring high-spin metal complexes as an alternative platform to radicals for molecular probes in EPRI. A key inherent advantage of metal ions is that the unique electronic feature of large zero-field splitting enables the possibility of safe, low-frequency microwave use at high magnetic field. Hence, metal complexes with this feature could provide a completely new set of EPRI molecular imaging probes with capabilities unmatched by organic radicals. However, all of the basic EPR spectral properties of metal complexes with low frequency microwaves are unmapped. The Zadrozny lab will amend this knowledge gap. The work will use synthetic inorganic chemistry and spectroscopic analyses to (1) understand how to target the frequency/field of the resonance to match the magnetic fields of MRI scanners with low-frequency microwaves (2) understand how to control the linewidth of the low-frequency EPR resonances to enhance resolution, and (3) how to merge radical/metal chemistry in hybrid molecules to gain the advantages of both metals and radicals for a single molecular probe system. Meeting these objectives will provide a new class of imaging probe capable of mapping physiological chemistry in a conventional MRI scanner.