'Omics from Source to Sink: Microbiome of a Stream Irrigation System and its Potential Impact

“从源头到汇的组学：溪流灌溉系统的微生物组及其潜在影响

• 批准号: 10712776
• 负责人: Mohammad Arif
• 金额: $ 18.26万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10712776
• 关键词: Address; Agriculture; Agrochemicals; Amendment; Annual Reports; Antibiotic Resistance; Centers of Research Excellence; Consumption; Core Facility; Creativeness; Data; Ecology; Ecosystem; Enteral; Environment; Environmental Health; Escherichia coli; Farm; Genomic approach; Genomics; Goals; Hawaii; Hazard Identification; Health; Human; Impairment; Infection; Irrigation; Knowledge; Laboratories; Link; Location; Mentors; Metagenomics; Methods; Microbe; Mission; Modeling; Modernization; Outcome; Pattern; Plants; Play; Pollution; Proliferating; Reporting; Research; Risk; Risk Factors; Role; Salmonella enterica; Sampling; Sea; Seasons; Sequence Analysis; Shotguns; Source; Stream; Structure; Study models; System; Tissues; United States; United States National Institutes of Health; Universities; Vegetables; Water; Work; career development; contaminated water; enteric pathogen; foodborne; foodborne illness; foodborne pathogen; human pathogen; imaging facilities; improved; in vivo; innovation; microbial; microbial community; microbial composition; microbial genomics; microbiome; microbiome composition; microscopic imaging; new technology; pathogen; resistance gene; sound; success; transmission process

PROJECT SUMMARY- ‘Omics from Source to Sink: Microbiome of a Stream Irrigation System and Its Potential Impact Food-borne illnesses associated with consumption of contaminated fresh produce are widely reported in the United States. Despite the recognized importance of irrigation water, a substantial unexplored gap exists in the knowledge of microbial communities present in irrigation systems and their potential roles in pathogen transmission and infection. This proposal addresses the critical need to determine potential microbial risk factors for human and environmental health and aligns with the COBRE mission of “how environmental microbes impact human health” as it aims to use ‘omics approaches to define relationships between environmental microbiome and its potential impacts on human health. Soft rot pectinolytic bacterial plant pathogens (SRP) macerate plant tissues of vegetable crops, producing a niche for foodborne human pathogens (FBP) and enabling the latter to colonize fresh produce. Our specific aims are: (I) Determine the composition and structure of the microbiomes of stream irrigation systems by amplicon and shotgun sequence analyses, and (II) determine the role that microbes in irrigation water play in establishing niches for FBP by internalization and in vivo interspecies interactions. Application of new technologies will permit more precise identification of microbial communities and results of this study will elucidate the interactions between SRP and FBP when co-infecting a plant host. The proposed research is creative and original in its genomic approach and provides a sound model for studying associations of FBP with edible crops grown in many other locations. Knowledge generated by this research will contribute not only to understanding the general ecology of microbial communities, but more specifically to dynamics of FBP and their interactions with SRP. Our projected results are expected to have important positive impacts on improving our understanding of evaluating risk factors and help to resolve poorly understood mechanisms. This impact aligns with NIH’s mission to seek fundamental knowledge to enhance health and reduce illness.

项目总结-“从源头到汇的组学：溪流灌溉系统及其微生物组学”